A New Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

Becatti, Matteo; Fucci, Rossella; Mannucci, Amanda; Barygina, Victoria; Mugnaini, Marco; Criscuoli, Luciana; Giachini, Claudia; Bertocci, Francesco; Picone, Rita; Evangelisti, Paolo; Rizzello, Francesca; Cozzi, C.; Taddei, Niccolò; Fiorillo, Claudia; Coccia, Maria Elisabetta

doi:10.20944/preprints201801.0021.v1

Cited by 8 publications

(1 citation statement)

References 44 publications

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“…A number of theories have been put forward one after another to explain the potential mechanism of ovarian aging, the most classic of which is the free radical theory, which maintains that oxidative stress caused by the increase of intracellular reactive oxygen species (ROS) is the main reason for mammalian cell senescence [20,21,22,23]. In addition, many studies have shown that oxidative stress initiates the ovarian aging process and other processes that promote ovarian aging, such as inflammation, mitochondrial dysfunction, mitochondrial depolarization, endogenous apoptosis and so on [24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of quercetin against H 2 O 2 induced KGN cells injury

Zhang

Shen

Wang

et al. 2023

Preprint

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Background Oxidative stress is one of the key contributors to cellular senescence and aging in mammals. Quercetin is a kind of bioactive flavonoid widely existing in medicinal plants and food. It has a variety of physiological activities such as antioxidant. Given that hydrogen peroxide (H2O2), as a strong oxidant, can easily diffuse through the cell membrane and cause oxidative damage to cells, the present study is designed to verify the protective effect of quercetin on ovarian granulosa cells under oxidative stress. Methods Cell viability and toxicity were examined by Cell Counting Kit-8 (CCK-8) and Lactate Dehydrogenase (LDH) assays. Reactive oxygen species (ROS) accumulation was detected by flow cytometry. Glutathione (GSH) level was measured to analyze the oxidation resistance. Cell apoptosis was evaluated by Hoechst 33258 staining, Acridine Orange (AO)/Ethidium Bromide (EB) staining and western blot. The mitochondrial structure was observed under a transmission electron microscope. Mitochondrial membrane integrity was detected by JC-1 staining and western blot. Results The results of the present study indicated that H2O2 could induce cell damage, promote ROS accumulation, and lead to GSH depletion. Further studies demonstrated that H2O2 resulted in mitochondrial morphological damage and depolarization, which activate caspase3/9 subsequently. However, quercetin could mitigate the damages. Conclusions Present study found that H2O2 induced oxidative stress and mitochondrial apoptosis of human ovarian granulosa tumor cell line (KGN), which could be attenuated by quercetin.

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Section: Introductionmentioning

confidence: 99%

Protective effects of quercetin against H 2 O 2 induced KGN cells injury

Zhang

Shen

Wang

et al. 2023

Preprint

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Altered redox status may contribute to aberrant folliculogenesis and poor reproductive outcomes in women with polycystic ovary syndrome

Naigaonkar

Dadachanji

Hinduja

et al. 2021

J Assist Reprod Genet

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An Insight into Giant Cell Arteritis Pathogenesis: Evidence for Oxidative Stress and SIRT1 Downregulation

et al. 2021

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Giant cell arteritis (GCA), medium and large vessel granulomatous vasculitis affecting the elderly, is characterized by a multitude of vascular complications, including venous thrombosis, myocardial infraction and stroke. The formation of granulomatous infiltrates and the enhanced accumulation of proinflammatory cytokines are typical features of this condition. The GCA pathogenesis remains largely unknown, but recent studies have suggested the involvement of oxidative stress, mainly sustained by an enhanced reactive oxygen species (ROS) production by immature neutrophils. On this basis, in the present study, we intended to evaluate, in GCA patients, the presence of systemic oxidative stress and possible alterations in the expression level of nuclear sirtuins, enzymes involved in the inhibition of inflammation and oxidative stress. Thirty GCA patients were included in the study and compared to 30 healthy controls in terms of leukocyte ROS production, oxidative stress and SIRT1 expression. Our results clearly indicated a significant increase (p < 0.05) both in the ROS levels in the leukocyte fractions and plasma oxidative stress markers (lipid peroxidation and total antioxidant capacity) in the GCA patients compared to the healthy controls. In PBMCs from the GCA patients, a significant decrease in SIRT1 expression (p < 0.05) but not in SIRT6 and SIRT7 expression was found. Taken together, our preliminary findings indicate that, in GCA patients, plasma oxidative stress is paralleled by a reduced SIRT1 expression in PBMC. Further studies are needed to highlight if and how these alterations contribute to GCA pathogenesis.

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A New Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

Cited by 8 publications

References 44 publications

Protective effects of quercetin against H 2 O 2 induced KGN cells injury

Protective effects of quercetin against H 2 O 2 induced KGN cells injury

Altered redox status may contribute to aberrant folliculogenesis and poor reproductive outcomes in women with polycystic ovary syndrome

An Insight into Giant Cell Arteritis Pathogenesis: Evidence for Oxidative Stress and SIRT1 Downregulation

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