A new BET inhibitor, 171, inhibits tumor growth through cell proliferation inhibition more than apoptosis induction

Damaneh, Mohammadali Soleimani; Hu, Jianping; Huan, Xia‐Juan; Song, Shanshan; Tian, Changqing; Chen, Dan-Qi; Meng, Tao; Chen, Yue‐Lei; Shen, Jingkang; Xiong, Bing; Wang, Ying-Qing

doi:10.1007/s10637-019-00818-z

Cited by 4 publications

(1 citation statement)

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“…Inhibition of proliferation of tumor cells is often accompanied by cell apoptosis. Based on the inhibition of proliferation of HCT-116 cells by peiminine, a further study on the effect of peiminine on cell apoptosis was carried out [16]. Flow cytometric analysis with Annexin V/PI showed that the percentage apoptosis of HCT-116 cells treated with peiminine was markedly increased, relative to control cells, indicating that peiminine enhanced apoptosis of HCT-116 cells.…”

Section: Discussionmentioning

confidence: 99%

Peiminine regulates the biological characteristics of colorectal cancer cells via P13K/Akt/mTOR and oxidative stress pathways

Jin¹,

Zhang²,

Fen³

et al. 2022

Trop. J. Pharm Res

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Purpose: To investigate the influence of peiminine on biological characteristics of colorectal cancer cells, and the underlying mechanism.Methods: Two groups of cultured human colorectal cancer HCT-116 cells were used: peiminine and control groups. Peiminine group cells were exposed to the drug at a final concentration of 100 μmol/L. The effect of peiminine on cell proliferation was determined with CCK-8 method, while its effect on apoptosis was determined with flow cytometric method. Cell migration was determined with scratch test. The effect of peiminine on the expressions of proteins associated with the P13K/Akt/mTOR pathway and Wnt/β-catenin pathway in HCT-116 cells was determined with Western blotting assay.Results: Cell proliferation was markedly reduced in the peiminine group, relative to control (p < 0.05). There was higher percentage cell apoptosis in peiminine-treated cells than in control. Moreover, cell migration potential was significantly lower in the peiminine-treated cells. There were significantly downregulated levels of p-P13K, p-Akt and p-mTOR expressions in peiminine group, relative to the corresponding control expressions (p < 0.05). However, there were significantly higher relative expression of Wnt in peiminine group than in control cells, but β-catenin level was reduced, relative to the corresponding control level (p < 0.05).Conclusion: These data indicate that peiminine suppresses the proliferative, apoptotic and migratory potential of colorectal carcinoma HCT-116 cells via regulation of P13K/Akt/mTOR and oxidative stress pathways.

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Section: Discussionmentioning

confidence: 99%