A new benchmark for time-resolved detection of singlet oxygen luminescence - revealing the evolution of lifetime in living cells with low dose illumination

Schlothauer, Jan C.; Hackbarth, Steffen; Röder, Beate

doi:10.1002/lapl.200810116

Cited by 75 publications

(54 citation statements)

References 35 publications

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“…In This is in agreement with a number of in vivo and in vitro studies [12][13][14][15][16][17][18][19][20][21].…”

Section: Autofluorescencesupporting

confidence: 92%

In Vitro Studies of Photosensitizer fluorescence changes on singlet oxygen mediated photobleaching

Atif

2013

J. Phys.: Conf. Ser.

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Abstract:The effects of photosensitizer concentration on the singlet oxygen mediated photobleaching have been investigated in keratinocytes. Cells having different photosensitizers concentration were identified on the basis of the fluorescence signal amplitude and their fluorescence decay then studied to see if there was a correlation with concentration. The experimental results shows that the photobleaching was more rapid at the higher concentration as compared to lower concentration which mean that singlet oxygen plays a vital role in the rapid m-tetrahydroxyphenylchlorin (mTHPC) induced fluorescence photobleaching causing the sensitizer to degrade.

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“…In This is in agreement with a number of in vivo and in vitro studies [12][13][14][15][16][17][18][19][20][21].…”

Section: Autofluorescencesupporting

confidence: 92%

In Vitro Studies of Photosensitizer fluorescence changes on singlet oxygen mediated photobleaching

Atif

2013

J. Phys.: Conf. Ser.

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“…The detection of singlet-oxygen luminescence in solution containing photosensitizers is meanwhile accomplished with a passable effort. Recently, several research groups were able to detect singlet-oxygen luminescence in lipids and even in living eukaryotic cells [9,[24][25][26][27].…”

Section: Resultsmentioning

confidence: 99%

A helpful technology – the luminescence detection of singlet oxygen to investigate photodynamic inactivation of bacteria (PDIB)

Regensburger

Maisch

Felgenträger

et al. 2010

Journal of Biophotonics

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Photodynamic inactivation of bacteria (PDIB) is considered a new approach for the struggle against multiresistant bacteria. To achieve a sufficient level of bacteria killing, the photosensitizer must attach to and/or penetrate the bacteria and generate a sufficiently high amount of singlet oxygen. To optimize PDIB, the direct detection and quantification of singlet oxygen in bacteria is a helpful tool. Singlet-oxygen luminescence is a very weak signal, in particular in living bacteria. We first performed experiments in aqueous photosensitizer solution to optimize the luminescence system. We eliminated non-singlet-oxygen photons, which is important for the quantification of singlet oxygen and its rise and decay rates. This procedure is even more important when the laser excitation beam is scattered by bacteria (diameter 1 microm). In suspensions with both Gram-positive and Gram-negative bacteria we then clearly detected singlet oxygen by its luminescence and determined the respective rise and decay times. The decay times should provide an indication of localization of singlet oxygen and hence of the photosensitizer even in small bacteria.

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“…It is well-known that 1 O 2 plays a key role in both the apoptotic and necrotic pathways of cell death induced by PDT [21,22], yet the molecular mechanisms of PDT at cellular level are not fully understood. This explains the strong interest in studying the intracellular 1 O 2 diffusion by directly observing the generation and quenching of 1 O 2 , or by indirectly adding the 1 O 2 quenchers [23][24][25].…”

Section: Imaging Ofmentioning

confidence: 93%

Indirect imaging of singlet oxygen generation from a single cell

et al. 2011

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Singlet oxygen (1 O 2 ) can be generated in a living cell upon focused laser irradiation of the intracellular photosensitizers.1 O2 lifetime in the living cells is shortened by the reactions with cellular molecules, and thus the 1 O2 diffusion in a single cell has attracted much attention. In this study, 1 O2 generation from the plasma membrane-targeted protoporphyrin IX (PpIX) and nuclear-targeted meso-Tetra (N-methyl-4-pyridyl) porphine tetra tosylate (TMPyP) in human nasopharyngeal carcinoma CNE2 cells was indirectly imaged by using a fluorescence probe Singlet Oxygen Sensor Green agent (SOSG), respectively. The confocal images indicate that the green fluorescence of SOSG in the vicinity of the PpIX-sensitized cells was dramatically enhanced with the increase of the irradiation time and intracellular PpIX, while there is no significant enhancement for the unsensitized and TMPyP-sensitized cells. The obtained results suggest that the 1 O2 generated from the plasma membrane-targeted PpIX in the CNE2 cells can escape into the extracellular medium and react with the SOSG to produce SOSG endoperoxides (SOSG-EP). Moreover, the fluorescence enhancement of SOSG mainly depends on the subcellular localization and intracellular uptake of the photosensitizers. Depending on the site of 1 O2 generation, 1 O2 generated in the plasma membrane can escape from the cell interior into the extracellular environment, while the 1 O2 generated in the nucleus cannot. Our findings indicate that SOSG holds great promise for the indirect imaging of the 1 O 2 that can escape from single intact living cells. The average intensity of four regions (marked as A, B, C, and D) from special time point was followed in each sample using the image-guided spectral analysis function

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A new benchmark for time-resolved detection of singlet oxygen luminescence - revealing the evolution of lifetime in living cells with low dose illumination

Cited by 75 publications

References 35 publications

In Vitro Studies of Photosensitizer fluorescence changes on singlet oxygen mediated photobleaching

In Vitro Studies of Photosensitizer fluorescence changes on singlet oxygen mediated photobleaching

A helpful technology – the luminescence detection of singlet oxygen to investigate photodynamic inactivation of bacteria (PDIB)

Indirect imaging of singlet oxygen generation from a single cell

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