A NEW BACTERIOPHAGE TYPING SET FOR <i>PSEUDOMONAS AERUGINOSA</i>

Bergan, Tom

doi:10.1111/j.1699-0463.1972.tb00149.x

Cited by 4 publications

(2 citation statements)

References 27 publications

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“… a. Bergan (1972) tested phage F8 for suitability as a typing phage and suggested that it was isolated by Postic and Finland (1961), but there is nothing in this paper to support this. …”

Section: Resultsmentioning

confidence: 90%

Comparative analysis of the widespread and conserved PB1‐like viruses infecting Pseudomonas aeruginosa

Ceyssens

Miroshnikov

Mattheus

et al. 2009

Environmental Microbiology

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SummaryWe examined the genetic diversity of lytic Pseudomonas aeruginosa bacteriophage PB1 and four closely related phages (LBL3, LMA2, 14-1 and SN) isolated throughout Europe. They all encapsulate linear, nonpermuted genomes between 64 427 and 66 530 bp within a solid, acid-resistant isometric capsid (diameter: 74 nm) and carry non-flexible, contractile tails of approximately 140 nm. The genomes are organized into at least seven transcriptional blocks, alternating on both strands, and encode between 88 (LBL3) and 95 (LMA2) proteins. Their virion particles are composed of at least 22 different proteins, which were identified using mass spectrometry. Posttranslational modifications were suggested for two proteins, and a frameshift hotspot was identified within ORF42, encoding a structural protein. Despite large temporal and spatial separations between phage isolations, very high sequence similarity and limited horizontal gene transfer were found between the individual viruses. These PB1-like viruses constitute a new genus of environmentally very widespread phages within the Myoviridae.

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“… a. Bergan (1972) tested phage F8 for suitability as a typing phage and suggested that it was isolated by Postic and Finland (1961), but there is nothing in this paper to support this. …”

Section: Resultsmentioning

confidence: 90%

Comparative analysis of the widespread and conserved PB1‐like viruses infecting Pseudomonas aeruginosa

Ceyssens

Miroshnikov

Mattheus

et al. 2009

Environmental Microbiology

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“…Phage F8 is one of the Pseudomonas typing phages from the Lindberg set which includes six more similar phages [93,94]. It possesses a 70-nm wide head with visible capsomers and a 138 nm-long tail, four short straight tail fibers and a base plate that separates from the sheath upon contraction.…”

Section: Resultsmentioning

confidence: 99%

Classification of Myoviridae bacteriophages using protein sequence similarity

et al. 2009

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BackgroundWe advocate unifying classical and genomic classification of bacteriophages by integration of proteomic data and physicochemical parameters. Our previous application of this approach to the entirely sequenced members of the Podoviridae fully supported the current phage classification of the International Committee on Taxonomy of Viruses (ICTV). It appears that horizontal gene transfer generally does not totally obliterate evolutionary relationships between phages.ResultsCoreGenes/CoreExtractor proteome comparison techniques applied to 102 Myoviridae suggest the establishment of three subfamilies (Peduovirinae, Teequatrovirinae, the Spounavirinae) and eight new independent genera (Bcep781, BcepMu, FelixO1, HAP1, Bzx1, PB1, phiCD119, and phiKZ-like viruses). The Peduovirinae subfamily, derived from the P2-related phages, is composed of two distinct genera: the "P2-like viruses", and the "HP1-like viruses". At present, the more complex Teequatrovirinae subfamily has two genera, the "T4-like" and "KVP40-like viruses". In the genus "T4-like viruses" proper, four groups sharing >70% proteins are distinguished: T4-type, 44RR-type, RB43-type, and RB49-type viruses. The Spounavirinae contain the "SPO1-"and "Twort-like viruses."ConclusionThe hierarchical clustering of these groupings provide biologically significant subdivisions, which are consistent with our previous analysis of the Podoviridae.

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Epidemiological typing ofPseudomonas aeruginosa

1988

Eur. J. Clin. Microbiol. Infect. Dis.

100

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Current knowledge of the typing of Pseudomonas aeruginosa and new methods for characterizing strains are reviewed. A combination of serotyping as a primary screen with pyocin typing for finer discrimination between isolates gives valid epidemiological data, is within the scope of most clinical laboratories and is to be recommended. Phage typing and H typing do provide good discrimination but are not reproducible in practice because a reaction-difference rule has to be applied to phage patterns, and diphasic variation reduces the accuracy of the identification of flagellar antigens. A problem remains with cystic fibrosis isolates, which show considerable heterogeneity in surface properties. For these strains pyocin production typing by the revised method of Govan is indicated. Newer techniques such as isoenzyme profile and DNA probes of the chromosome require independent evaluation and due to their technical difficulty may not be adopted in a routine context for some years to come.

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A NEW BACTERIOPHAGE TYPING SET FOR PSEUDOMONAS AERUGINOSA

Cited by 4 publications

References 27 publications

Comparative analysis of the widespread and conserved PB1‐like viruses infecting Pseudomonas aeruginosa

Comparative analysis of the widespread and conserved PB1‐like viruses infecting Pseudomonas aeruginosa

Classification of Myoviridae bacteriophages using protein sequence similarity

Epidemiological typing ofPseudomonas aeruginosa

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