A New Bacterial Strain Alcaligenes aquatilis MAG 1 Associated with the Mediterranean Sea Lithophaga (Bivalvia) Precipitating in Nutrient Free Medium Shell-Like Calcium Carbonate Polymorphs

Mehanni, Magda M.; El-Aziz, Shaban H. Abd

doi:10.21608/eajbsg.2019.153533

Cited by 2 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nucleotide sequence of the forward primer (27F) is 5 -AGAG ⊤ TGATCMTGGCTCAG -3 and the reverse primer (1492R) is 5 -TACGGYTAℂ ⊤ G ⊤ ACGAC ⊤ -3 [56]. Ampli cation was performed in 50 µL reaction volumes as described in Mehanni and Abd El-Aziz, 2019 [57].…”

Section: Harvesting Of Prostatic Secretionsmentioning

confidence: 99%

Isolation, characterization, and antibiotic sensitivity pattern of Gram-negative bacteria causing chronic bacterial prostatitis

Mehanni

Sayed

El-Gendy

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Bacterial prostatitis (BP) is an infection of the prostate gland, it can be acute (ABP) or chronic (CBP) and if not treated appropriately, it can result in significant morbidity.Results: In this study, expressed prostate secretion from 10 patients that are diagnosed with chronic bacterial prostatitis at Ain-Shams hospital, Egypt are used to isolate the common causative bacteria for CBP. Results of this study indicated that Gram negative bacteria were predominant since 54 Gram-ve bacterial isolates were obtained out of the total 62 isolates. Gram-ve bacterial isolates were grouped according to their oxidase test results and to lactose fermentation capability into three groups. The largest group contained 38 isolates of oxidase negative, lactose fermenters. Five representative isolates of this group were identified biochemically or by 16S rRNA sequence homology to be related to Enterobacter cloacae complex (3 Enterobacter cloacae related strains and 2 Enterobacter kobei related strains). Oxidase positive lactose non-fermentors group contained 9 isolates while oxidase negative lactose non-fermentors group contained 7 isolates. A representative isolate for each of them were identified by 16S rRNA sequence homology to be a strain of Pseudomonas stutzeri and Providencia stuartii respectively. Antibiotic sensitivity pattern of the chosen representative isolates indicated that all of them were sensitive to ciprofloxacin followed by ampicillin while resistance to cefaclor and aztereonam was prevalent. Other tested antibiotics recorded different sensitivity pattern among tested isolates.Conclusions: isolates of Genus Enterobacter sp. included in the E. cloacae complex group represent the most common bacterial pathogen in tested EPS of CBP diagnosed patients, of them Enterobacter cloacae was the most common.

show abstract

Section: Harvesting Of Prostatic Secretionsmentioning

confidence: 99%

Isolation, characterization, and antibiotic sensitivity pattern of Gram-negative bacteria causing chronic bacterial prostatitis

Mehanni

Sayed

El-Gendy

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…faecalis, A.faecalis subsp. parafaecalis, this genus, three other species are described, namely, A. pakistanensis, A. aquatilis, and A. endophyticus (Mehanni et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing of Alcaligenes sp. strain MMA: insight into the antibiotic and heavy metal resistant genes

et al. 2023

View full text Add to dashboard Cite

Introduction: A wide range of pollutants, including the likes of xenobiotics, heavy metals, and antibiotics, are characteristic of marine ecosystems. The ability of the bacteria to flourish under high metal stress favors the selection of antibiotic resistance in aquatic environments. Increased use and misuse of antibiotics in medicine, agriculture, and veterinary have posed a grave concern over antimicrobial resistance. The exposure to these heavy metals and antibiotics in the bacteria drives the evolution of antibiotic and heavy metal resistance genes. In the earlier study by the author Alcaligenes sp. MMA was involved in the removal of heavy metals and antibiotics. Alcaligenes display diverse bioremediation capabilities but remain unexplored at the level of the genome.Methods: To shed light on its genome, the Alcaligenes sp. strain MMA, was sequenced using Illumina Nova Seq sequencer, which resulted in a draft genome of 3.9 Mb. The genome annotation was done using Rapid annotation using subsystem technology (RAST). Given the spread of antimicrobial resistance and the generation of multi-drug resistant pathogens (MDR), the strain MMA was checked for potential antibiotic and heavy metal resistance genes Further, we checked for the presence of biosynthetic gene clusters in the draft genome.Results:Alcaligenes sp. strain MMA, was sequenced using Illumina Nova Seq sequencer, which resulted in a draft genome of 3.9 Mb. The RAST analysis revealed the presence of 3685 protein-coding genes, involved in the removal of antibiotics and heavy metals. Multiple metal-resistant genes and genes conferring resistance to tetracycline, beta-lactams, and fluoroquinolones were present in the draft genome. Many types of BGCs were predicted, such as siderophore. The secondary metabolites of fungi and bacteria are a rich source of novel bioactive compounds which have the potential to in new drug candidates.Discussion: The results of this study provide information on the strain MMA genome and are valuable for the researcher in further exploitation of the strain MMA for bioremediation. Moreover, whole-genome sequencing has become a useful tool to monitor the spread of antibiotic resistance, a global threat to healthcare.

show abstract

A New Bacterial Strain Alcaligenes aquatilis MAG 1 Associated with the Mediterranean Sea Lithophaga (Bivalvia) Precipitating in Nutrient Free Medium Shell-Like Calcium Carbonate Polymorphs

Cited by 2 publications

References 28 publications

Isolation, characterization, and antibiotic sensitivity pattern of Gram-negative bacteria causing chronic bacterial prostatitis

Isolation, characterization, and antibiotic sensitivity pattern of Gram-negative bacteria causing chronic bacterial prostatitis

Whole-genome sequencing of Alcaligenes sp. strain MMA: insight into the antibiotic and heavy metal resistant genes

Contact Info

Product

Resources

About