A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies

Solves, Pilar; Mirabet, Vicente; Blanquer, Amando; Delgado-Rosas, Francisco; Planelles, Dolores; Andrade, Margarita; Carbonell-Uberos, Francisco; Soler, María Rodríguez; Roig, Roberto

doi:10.1080/14653240903253865

Cited by 2 publications

(3 citation statements)

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“…Lauber et al [32] (Mannheim Cord Blood Bank). The recovery rate of TNCs was similar to that reported by Solves et al [33] at the Valencia Cord Blood Bank in Spain.…”

Section: Hla Typingsupporting

confidence: 74%

“…The quality of the product is reliant on both the procedures and processes at that time and the subsequent maintenance of proper storage conditions [27]. Reducing the volume of the UCB units by eliminating red blood cell bulk and plasma offers pragmatic advantages of convenience, efficiency, and cost [28,29]. We used a closed system to deplete red blood cells and plasma; the median volume and TNC content were similar to those of earlier studies, e.g.…”

Section: Hla Typingmentioning

confidence: 86%

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Cord Blood Banking and Transplantation in China: A Ten Years Experience of a Single Public Bank

Liu

Chen

et al. 2012

Transfus Med Hemother

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Background: Umbilical cord blood (UCB) has successfully used for transplantation to treat hematologic malignancies and genetic diseases. Herein, we describe the experience generated in a single public UCB bank at Zhejiang Province in China. Methods: Good manufacturing practice and standard operating procedures were used to address donor selection as well as UCB collection, processing, and cryopreservation. Total nucleated cells (TNCs), cellular viability, CD34+ cells, and colony-forming units were determined, and infectious diseases screening test, sterility test, and HLA typing for UCB units were done. Results: Only 18.51% of all collected UCB units met storage criteria, and 7,056 UCB units were cryopreserved in 10 years. The volume of UCB units was 95.0 ± 22.0 ml. The number of TNCs before and after processing was 13.32 ± 3.63 × 10⁸ and 10.63 ± 2.80 × 10⁸, respectively, and the recovery rate was 80.71 ± 11.26%. 0.4344 ± 0.1874% of the TNCs were CD34+ cells. The CFU-GM was 32.1 ± 28.0 colonies per 1 × 10⁵ nucleated cells. Based mainly on HLA and nucleated cell content, 26 UCB units were released for transplantation. Conclusions A public UCB bank was successfully established in China; collection and processing of UCB units should be optimized in order to gain maximum volume and cell count.

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“…Lauber et al [32] (Mannheim Cord Blood Bank). The recovery rate of TNCs was similar to that reported by Solves et al [33] at the Valencia Cord Blood Bank in Spain.…”

Section: Hla Typingsupporting

confidence: 74%

Section: Hla Typingmentioning

confidence: 86%

Cord Blood Banking and Transplantation in China: A Ten Years Experience of a Single Public Bank

Liu

Chen

et al. 2012

Transfus Med Hemother

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“…In addition, CB specimens are normally one-tenth the size of BM or PBSC specimens (~100 cc), simplifying the biobanking process, although as discussed in the next section, volume-reduction is routinely performed to further simplify the process using multiple manual and automated processes. [24][25][26][27] Over the past 20 years, more than 35,000 CB transplants have been performed worldwide and more than 5 million CB samples have been collected and cryopreserved 8 (www.bethematch.org).…”

Section: Umbilical Cord Bloodmentioning

confidence: 99%

Long-term frozen storage of stem cells: challenges and solutions

Harris¹

2016

BSAM

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Stem cells are found in all multicellular organisms and are defined as cells that can differentiate into specialized mature cells as well as divide to produce more stem cells. Stem cells are commonly harvested for clinical and research applications from bone marrow, peripheral blood, umbilical cord blood and tissue, and adipose tissue. These sites are easily accessible and economical to harvest and contain large numbers of stem cells. The advent of modern cryopreservation technology introduced the concept of harvesting and banking stem cells for future use to avoid issues with donor attrition. Large-scale stem cell banking really began in earnest in the 1990s with the establishment of umbilical cord blood banks, which gradually expanded to include cord tissue and finally adipose tissue. Banked stem cells of all origins not only are used for research but are commonly utilized for clinical applications that include transplantation and regenerative medicine. Many of these stem cell sources have been utilized after cryopreservation, which is the subject of this review. Often the stem cells are stored for varying periods of time which may range from weeks to years (even decades) and are often stored in multiple aliquots (which may vary in size) in order to make the stem cell samples more amenable to multiple uses. There is a considerable investment of time and money into these endeavors as it can directly impact patient safety. Each stem cell source has its own particular challenge(s), although there is some overlap, demanding its own particular requirements to achieve a solution. This review will cover the challenges and unique requirements involved in collection, processing, cryopreservation, storage, thawing, and quality control for each of the most commonly used stem cell sources (bone marrow, cord blood, mobilized peripheral blood stem cell, cord tissue, and adipose tissue).

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A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies

Cited by 2 publications

References 0 publications

Cord Blood Banking and Transplantation in China: A Ten Years Experience of a Single Public Bank

Cord Blood Banking and Transplantation in China: A Ten Years Experience of a Single Public Bank

Long-term frozen storage of stem cells: challenges and solutions

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