A new autologous keratinocyte dressing treatment for non‐healing diabetic neuropathic foot ulcers

Moustafa, Manar; Simpson, C.; Glover, Mark; Dawson, Rebecca; Tesfaye, Solomon; Creagh, F. M.; Haddow, D. B.; Short, Robert D.; Heller, Simon; MacNeil, Sheila

doi:10.1111/j.1464-5491.2004.01166.x

Cited by 91 publications

(62 citation statements)

References 16 publications

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“…28,29 Consistent with the observations of primary corneal epithelial cells here, AA-derived surfaces have previously been shown to promote keratinocyte attachment and a greater number of cells transferred onto deepithelialized dermis culture in vitro. 28 The presence of acid functional groups on a surface is known to promote cell attachment.…”

Section: Discussionsupporting

confidence: 74%

“…This has previously been demonstrated through the use of acidfunctionalized surfaces that promoted the growth of keratinocytes. 28,29 The surface concentration of the acid groups can be controlled by the addition of diluents to the plasma process, which, in turn, can affect how strongly cells adhere to these surfaces, allowing the cells to transfer from the surface onto a wound bed. 30 This study investigates the use of plasma polymer-coated contact lenses as substrates for in vitro culture and also as carriers for in vivo transfer of limbal epithelial cells in the treatment of LSCD.…”

Section: Introductionmentioning

confidence: 99%

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Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

Brown

Low

Mariappan

et al. 2014

Tissue Engineering Part A

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Extensive damage to the limbal region of the cornea leads to a severe form of corneal blindness termed as limbal stem cell deficiency (LSCD). Whereas most cases of corneal opacity can be treated with full thickness corneal transplants, LSCD requires stem cell transplantation for successful ocular surface reconstruction. Current treatments for LSCD using limbal stem cell transplantation involve the use of murine NIH 3T3 cells and human amniotic membranes as culture substrates, which pose the threat of transmission of animal-derived pathogens and donor tissue-derived cryptic infections. In this study, we aimed to produce surface modified therapeutic contact lenses for the culture and delivery of corneal epithelial cells for the treatment of LSCD. This approach avoids the possibility of suture-related complications and is completely synthetic. We used plasma polymerization to deposit acid functional groups onto the lenses at various concentrations. Each surface was tested for its suitability to promote corneal epithelial cell adhesion, proliferation, retention of stem cells, and differentiation and found that acid-based chemistries promoted better cell adhesion and proliferation. We also found that the lenses coated with a higher percentage of acid functional groups resulted in a higher number of cells transferred onto the corneal wound bed in rabbit models of LSCD. Immunohistochemistry of the recipient cornea confirmed the presence of autologous, transplanted 5-bromo-2¢-deoxyuridine (BrdU)-labeled cells. Hematoxylin staining has also revealed the presence of a stratified epithelium at 26 days post-transplantation. This study provides the first evidence for in vivo transfer and survival of cells transplanted from a contact lens to the wounded corneal surface. It also proposes the possibility of using plasma polymer-coated contact lenses with high acid functional groups as substrates for the culture and transfer of limbal cells in the treatment of LSCD.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

Brown

Low

Mariappan

et al. 2014

Tissue Engineering Part A

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“…The study of plasma polymer coatings that could potentially be used in biomedical devices is an area of increasing research interest [1][2][3][4][5]. One of the most common classes of thin film treatments employed in biomaterial devices is that of 'low-fouling' or 'stealth' coatings [6].…”

Section: Introductionmentioning

confidence: 99%

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

Menzies

Nelson

Shen

et al. 2011

J. R. Soc. Interface.

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Plasma-enhanced chemical vapour-deposited films of di(ethylene glycol) dimethyl ether were analysed by a combination of X-ray photoelectron spectroscopy, atomic force microscopy, quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray and neutron reflectometry (NR). The combination of these techniques enabled a systematic study of the impact of plasma deposition conditions upon resulting film chemistry (empirical formula), mass densities, structure and water solvation, which has been correlated with the films' efficacy against protein fouling. All films were shown to contain substantially less hydrogen than the original monomer and absorb a vast amount of water, which correlated with their mass density profiles. A proportion of the plasma polymer hydrogen atoms were shown to be exchangeable, while QCM-D measurements were inaccurate in detecting associated water in lower power films that contained loosely bound material. The higher protein resistance of the films deposited at a low load power was attributed to its greater chemical and structural similarity to that of poly(ethylene glycol) graft surfaces. These studies demonstrate the utility of using X-ray and NR analysis techniques in furthering the understanding of the chemistry of these films and their interaction with water and proteins.

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“…However, in addition to fibroblasts, keratinocytes, as the main epidermal cells, play a significant role in developing the outer skin layer. Therefore, after infiltration of fibroblasts and preparation of a suitable substrate, keratinocyte cells are used to obtain results closer to the conditions of natural skin (18).…”

Section: Application Of Fibroblasts In Diabetic Foot Ulcermentioning

confidence: 99%

Application of Dermal Fibroblast Cells in Cell Therapy of Wound

et al. 2017

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Context and Evidence Acquisition: Cell therapy, with autologous dermal fibroblasts, shows remarkable potential and is currently achieved by different methods, such as spraying, grafting, and injecting to treat various types of ulcers, wrinkles, acne scars, and surgical wounds. Results: To prepare fibroblasts for clinical use, some critical points must be taken into consideration: isolation and culture of these cells to produce a homogeneous population of non-contaminated cells, a method for rapid growth, and development of a proportional number of fibroblast cells with proper strength and viability. In addition, an appropriate site for a biopsy should be selected based on indications of treatment and maintenance. Conclusions: Ultimately, the clinical use of fibroblasts in wound healing obviates the need for ready to use reservoir of these cells. In this regard, dermal allografts can be used in both fresh and frozen forms. The fresh type is more desirable due to its high chance of survival. Developing the appropriate preservation methods for allogeneic fibroblasts is the emerging research field of interest. Furthermore, applying biomaterials will increase the efficacy of fibroblasts in wound healing.

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A new autologous keratinocyte dressing treatment for non‐healing diabetic neuropathic foot ulcers

Abstract: TranCell delivery of autologous cells is a promising treatment for chronic diabetic foot ulcers with no side-effects and no recurrence in the healed ulcers.

Cited by 91 publications

References 16 publications

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

Application of Dermal Fibroblast Cells in Cell Therapy of Wound

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