A new approach to study adsorbed proteins on biomaterials: Immunogold staining

Park, K; Albrecht, Ralph M.; Simmons, Scott R.; Cooper, Stuart L.

doi:10.1016/0021-9797(86)90019-6

Cited by 32 publications

(17 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…40 It has been shown experimentally that artificial surfaces preadsorbed with fibrinogen lead to higher platelet adhesion when compared with control surfaces. 41 Therefore, the improvement of antithrombogenicity of Hf-implanted NiTi accords well with the reduction of fibrinogen adsorption as shown in Figure 11. Similar results have been found in oxygen implanted NiTi, which exhibited a level of protein adhesion less than or equal to that of native NiTi.…”

Section: Cell-materials Interactionssupporting

confidence: 62%

Ni ion release, osteoblast–material interactions, and hemocompatibility of hafnium‐implanted NiTi alloy

Zhao

et al. 2011

J Biomed Mater Res

View full text Add to dashboard Cite

Hafnium ion implantation was applied to NiTi alloy to suppress Ni ion release and enhance osteoblast-material interactions and hemocompatibility. The auger electron spectroscopy, x-ray photoelectron spectroscopy, and atomic force microscope results showed that a composite TiO(2)/HfO(2) nanofilm with increased surface roughness was formed on the surface of NiTi, and Ni concentration was reduced in the superficial surface layer. Potentiodynamic polarization tests displayed that 4 mA NiTi sample possessed the highest E(br) - E(corr), 470 mV higher than that of untreated NiTi, suggesting a significant improvement on pitting corrosion resistance. Inductively coupled plasma mass spectrometry tests during 60 days immersion demonstrated that Ni ion release rate was remarkably decreased, for example, a reduction of 67% in the first day. The water contact angle increased and surface energy decreased after Hf implantation. Cell culture and methyl-thiazol-tetrazolium indicated that Hf-implanted NiTi expressed enhanced osteoblasts adhesion and proliferation, especially after 7 days culture. Hf implantation decreased fibrinogen adsorption, but had almost no effect on albumin adsorption. Platelets adhesion and activation were suppressed significantly (97% for 4 mA NiTi) and hemolysis rate was decreased by at least 57% after Hf implantation. Modified surface composition and morphology and decreased surface energy should be responsible for the improvement of cytocompatibility and hemocompatibility.

show abstract

Section: Cell-materials Interactionssupporting

confidence: 62%

Ni ion release, osteoblast–material interactions, and hemocompatibility of hafnium‐implanted NiTi alloy

Zhao

et al. 2011

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…In a static, unstirred, colloidal system with no or little agitation, as is the case in the VLM chamber, efficiency of labeling is dependent, in part, on the temperature, laws of Brownian motion, and affinity of ligand or antibody for the receptor. While labeling times on the live cells are necessarily short, it appears from our previous studies Goodman and Albrecht, 1987;Olorundare et al, 1987;Park et al, 1986a) and empirical observations that the use of these concentrated labels permits a relatively high efficiency of receptor labeling.…”

Section: Preparation Of Fibrinogen Fibrinogen-gold and Antibody-golmentioning

confidence: 91%

Distribution and movement of membrane‐associated platelet glycoproteins: Use of colloidal gold with correlative video‐enhanced light microscopy, low‐voltage high‐resolution scanning electron microscopy, and high‐voltage transmission electron microscopy

1989

View full text Add to dashboard Cite

Scanning electron microscopy (SEM), especially low-voltage (1 KeV) high-resolution SEM, can be used in conjunction with stereo pair high-voltage (1 MeV) transmission electron microscopy (HVEM) of whole spread cells or thick sections effectively to correlate surface structure with internal structure. Surface features such as microvilli, pits, pseudopodia, ruffles, attached virus, and other surface-related morphologic characteristics can be identified using SEM, while underlying cytoskeletal structure and organelle organization can be viewed by HVEM of the same preparation. However, the need to "prepare" cells for electron microscopy precludes observation in the living state. The use of several types of video-enhanced light microscopy (VLM) permits observation of living cells such that certain surface and internal features can be observed at a relatively high level of resolution or detection. Thus, changes in living cells can be followed, and at appropriate times the cells may be chemically fixed or rapidly frozen and prepared for ultrastructural examination by electron microscopy. We have utilized VLM in conjunction with SEM and HVEM to correlate changes in shape and surface structure with changes in the internal structure of platelets. In addition, we have found it advantageous to use colloidal gold-labeling procedures, because these markers are detectable by all three forms of microscopy. Using this approach we have labeled platelet membrane GPIIb/IIIa, a receptor for RGD-containing adhesive proteins, with gold-fibrinogen or gold-anti-IIb/IIIa. The initial binding and subsequent movement of gold-fibrinogen-IIb/IIIa complexes in living platelets was followed by VLM. The movement of individual labels could be mapped. Subsequent observation by low-voltage (1 KeV) high-resolution SEM and HVEM permits visualization of the same individual receptors tracked by LM. The final position on the membrane or the position-in-transit when fixative was added was determined relative to surface ultrastructure (SEM) and internal, particularly cytoskeletal, ultrastructure (HVEM).

show abstract

“…Surface modification typically follows two approaches. The first one is to generate hydroxylterminated monolayers [195,198,199] and the second one is to utilize the chemical or physical adsorption of phospholipids or phospholipid derivatives on alkylsilane monolayers [196,197,[200][201][202][203]. Protein adsorption and cell adhesion are reduced on the phosphorylcholine-assembled surfaces.…”

Section: Biochemical Modification Of Titanium and Titanium Alloysmentioning

confidence: 99%

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Liu

Chu

Ding

2004

Materials Science and Engineering: R: Reports

3,039

1,477

View full text Add to dashboard Cite

Titanium and titanium alloys are widely used in biomedical devices and components, especially as hard tissue replacements as well as in cardiac and cardiovascular applications, because of their desirable properties, such as relatively low modulus, good fatigue strength, formability, machinability, corrosion resistance, and biocompatibility. However, titanium and its alloys cannot meet all of the clinical requirements. Therefore, in order to improve the biological, chemical, and mechanical properties, surface modification is often performed. This article reviews the various surface modification technologies pertaining to titanium and titanium alloys including mechanical treatment, thermal spraying, sol-gel, chemical and electrochemical treatment, and ion implantation from the perspective of biomedical engineering. Recent work has shown that the wear resistance, corrosion resistance, and biological properties of titanium and titanium alloys can be improved selectively using the appropriate surface treatment techniques while the desirable bulk attributes of the materials are retained. The proper surface treatment expands the use of titanium and titanium alloys in the biomedical fields. Some of the recent applications are also discussed in this paper. #

show abstract

A new approach to study adsorbed proteins on biomaterials: Immunogold staining

Cited by 32 publications

References 28 publications

Ni ion release, osteoblast–material interactions, and hemocompatibility of hafnium‐implanted NiTi alloy

Ni ion release, osteoblast–material interactions, and hemocompatibility of hafnium‐implanted NiTi alloy

Distribution and movement of membrane‐associated platelet glycoproteins: Use of colloidal gold with correlative video‐enhanced light microscopy, low‐voltage high‐resolution scanning electron microscopy, and high‐voltage transmission electron microscopy

Surface modification of titanium, titanium alloys, and related materials for biomedical applications

Contact Info

Product

Resources

About