A new approach to man-machine communication for computerized microscopy

This paper reports on cellular sociology, which consists of modeling tissular architecture based on graph theory. Voronoi's diagram was chosen to build the models. This diagram derives from a cell neighborhood concept and generates parameters which objectively represent tissue architecture. Minimal spanning tree (MST) is probably the more frequently used among graphs and successfully discriminates different grades of pathological process. However, Voronoi's diagram is more comprehensive and a more complete representation of architecture with the advantage of stability. The lung neuroendocrine tumor classification is far from being consensual, especially for lesions which don't fall in with typical carcinoid and small cell carcinoma groups. By comparing architectural models of 20 neuroendrocrine tumors of the lung, this work supports the morphologic spectrum concept of these tumors and also supports the recently proposed concept of large‐cell neuroendocrine tumors of the lung. Finally, architectural parameters separate small‐cell‐lung carcinomas from neuroendocrine non‐small‐cell lung carcinomas. © 1996 Wiley‐Liss, Inc.

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Cellular sociology applied to neuroendocrine tumors of the lung: quantitative model of neoplastic architecture

Bigras

Marcelpoil

Brambilla

et al. 1996

Cytometry

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Le microscope HOME : un outil pour la qualité du dépistage du cancer du col utérin

Morens

Krief

Brugal

1995

Revue Française des Laboratoires

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Computer-assisted quantification of axo-somatic boutons at the cell membrane of motoneurons

Bredno

Metzler

Brook

et al. 2001

IEEE Trans. Biomed. Eng.

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This paper presents a system for computer-assisted quantification of axo-somatic boutons at motoneuron cell-surface membranes. Different immunohistochemical stains can be used to prepare tissue of the spinal cord. Based on micrographs displaying single neurons, a finite element balloon model has been applied to determine the exact location of the cell membrane. A synaptic profile is extracted next to the cell membrane and normalized with reference to the intracellular brightness. Furthermore, a manually selected reference cell is used to normalize settings of the microscope as well as variations in histochemical processing for each stain. Thereafter, staining, homogeneity, and allocation of boutons are determined automatically from the synaptic profiles. The system is evaluated by applying the coefficient of variation (Cv) to repeated measurements of a quantity. Based on 1856 motoneuronal images acquired from four animals with three stains, 93% of the images are analyzed correctly. The others were rejected, based on process protocols. Using only rabbit anti-synaptophysin as primary antibody, the correctness increases above 96%. Cv values are below 3%, 5%, and 6% for all measures with respect to stochastic optimization, cell positioning, and a large range of microscope settings, respectively. A sample size of about 100 is required to validate a significant reduction of staining in motoneurons below a hemi-section (Wilcoxon rank-sum test, alpha = 0.05, beta = 0.9). Our system yields statistically robust results from light micrographs. In future, it is hoped that this system will substitute for the expensive and time-consuming analysis of spinal cord injury at the ultra-structural level, such as by manual interpretation of nonoverlapping electron micrographs.

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A new approach to man-machine communication for computerized microscopy

Cited by 3 publications

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Cellular sociology applied to neuroendocrine tumors of the lung: quantitative model of neoplastic architecture

Cellular sociology applied to neuroendocrine tumors of the lung: quantitative model of neoplastic architecture

Le microscope HOME : un outil pour la qualité du dépistage du cancer du col utérin

Computer-assisted quantification of axo-somatic boutons at the cell membrane of motoneurons

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