A New Approach to Anodic Substitution Reaction Using Parallel Laminar Flow in a Micro-Flow Reactor

Synthetic Organic Electrochemical Methods Since 2000: On the Verge of a Renaissance

“…136,137 When the carbamate and the nucleophile are introduced in different streams, mass transfer between these separate streams can only occur via diffusion between the liquid–liquid contact area. Due to the small size of the flow channel, this area will remain stable and laminar.…”

The combined use of a continuous‐flow microchannel reactor and ionic liquid cosolvent for efficient biocatalysis of unpurified recombinant enzyme

“…The main channel dimension was 200 μm wide, 100 μm deep and 1 m long, and the channel of the microflow reactor was small enough to ensure that the flow regime is stable and laminar. 25 The reaction substrate at 0.01 g L -1 (rutin at pH 5.0 in citric acid-disodium hydrogen phosphate) and the recombinant RhaB1 solution containing 0.02 g mL -1 [Toma][Tf 2 N] were both pumped into the microchannel by a two-channel syringe pump. A heater (DC-230C, Tianjin Dongkang Technology Co., Ltd, China) was used to control reaction temperature and a digital thermometer was used to measure the temperature of receptor fluid.…”

“…In addition, compared with the batch reactor, the k cat and v max /K m were improved 19.2 and 65.7 times, respectively, which indicated a more efficient reaction between the enzyme and substrate in the microchannel reactor. 25 Typically, the apparent Michaelis constant depends on two factors, the enzyme and the mass transfer rate. The different Michaelis constant suggested that the apparent kinetics of the different reactors were significantly affected by the mass transfer.…”

“…Compared with a continuous-flow reactor, the reaction rate was increased 4-fold. 25 However, the hesperidinase produced by Aspergillus niger was purchased from Sigma Chemical Co., it was very expensive and contained -L-rhamnosidase and -D-glucosidase activity, with the presence of the latter being responsible for a quercetin byproduct. In the present study, recombinant RhaB1 was used in the microchannel reactor with one T-shaped inlet and outlet, and the maximum isoquercitrin yield (99.3 ± 4.3%) was achieved in 10 min at 35 ∘ C at 2 μL min -1 , the productivity was improved 61.4 times The cost of RhaB1 extracted from recombinant BL21-pET21a-rhaB1 assisted by ultrasound, was much lower than the cost of hesperidinase, which would be good for industrial production.…”

Electrochemical Reactions in Microreactors

“…A parallel laminar flow enabled the effective generation of an N-acyliminium ion followed by trapping with allyltrimethylsilane, which might be easily oxidized under homogeneous conditions (Figure 9.9) [28]. A solution of a carbamate and a solution of allyltrimethylsilane were introduced into the reactor in a parallel manner.…”