A new approach for turn-on fluorescence sensing of l-DOPA

Wang, Lu; Su, Dongdong; Berry, Stuart N.; Lee, Jungyeol; Chang, Young‐Tae

doi:10.1039/c7cc07640a

Cited by 22 publications

(9 citation statements)

References 34 publications

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“…Our overall findings regarding the effects of a signaling molecule on multiple pathogenic features found in AD, along with detailed mechanisms, could provide a novel and broad insight into distinct chemistry of neurotransmitters under physiological and pathogenic conditions. Such molecular-level approaches, including detailed mechanistic studies and utilization of chemical reagents, could further elucidate complex biological networks. ,− …”

Section: Introductionmentioning

confidence: 99%

Regulatory Activities of Dopamine and Its Derivatives toward Metal-Free and Metal-Induced Amyloid-β Aggregation, Oxidative Stress, and Inflammation in Alzheimer’s Disease

Nam

Derrick

Lee

et al. 2018

ACS Chem. Neurosci.

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A catecholamine neurotransmitter, dopamine (DA), is suggested to be linked to the pathology of dementia; however, the involvement of DA and its structural analogues in the pathogenesis of Alzheimer's disease (AD), the most common form of dementia, composed of multiple pathogenic factors has not been clear. Herein, we report that DA and its rationally designed structural derivatives (1-6) based on DA's oxidative transformation are able to modulate multiple pathological elements found in AD [i.e., metal ions, metal-free amyloid-β (Aβ), metal-bound Aβ (metal-Aβ), and reactive oxygen species (ROS)], with demonstration of detailed molecular-level mechanisms. Our multidisciplinary studies validate that the protective effects of DA and its derivatives on Aβ aggregation and Aβ-mediated toxicity are induced by their oxidative transformation with concomitant ROS generation under aerobic conditions. In particular, DA and the derivatives (i.e., 3 and 4) show their noticeable anti-amyloidogenic ability toward metal-free Aβ and/or metal-Aβ, verified to occur via their oxidative transformation that facilitates Aβ oxidation. Moreover, in primary pan-microglial marker (CD11b)-positive cells, the major producers of inflammatory mediators in the brain, DA and its derivatives significantly diminish inflammation and oxidative stress triggered by lipopolysaccharides and Aβ through the reduced induction of inflammatory mediators as well as upregulated expression of heme oxygenase-1, the enzyme responsible for production of antioxidants. Collectively, we illuminate how DA and its derivatives could prevent multiple pathological features found in AD. The overall studies could advance our understanding regarding distinct roles of neurotransmitters in AD and identify key interactions for alleviation of AD pathology.

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Section: Introductionmentioning

confidence: 99%

Regulatory Activities of Dopamine and Its Derivatives toward Metal-Free and Metal-Induced Amyloid-β Aggregation, Oxidative Stress, and Inflammation in Alzheimer’s Disease

Nam

Derrick

Lee

et al. 2018

ACS Chem. Neurosci.

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“…23 Fluorescent turn-on sensors have special advantages over turn-off sensors because the former involved a lower limit of detection (LOD), less capability of showing interference, maintaining a high signal-tobackground ratio, and excellent selectivity. 24 Recently, smartphone imaging analytical techniques achieved considerable attention in the field of sensors. 25−27 Smartphone devices mostly satisfy the requirement of portability, easy to operate through their different software applications, costeffectiveness in fabrication, and high sensitivity in real-time application.…”

Section: ■ Introductionmentioning

confidence: 99%

Smart-Phone-Assisted Optical Biosensors Based on Silk-Fibroin-Decorated Reduced Graphene Oxide Quantum Dots for Fluorescent Turn-On Recognition of l-Dopa

Goswami

Boruah

Sarma

2023

ACS Appl. Nano Mater.

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Levodopa (l-dopa) is a gold standard neurological drug for symptomatic medication of Parkinson’s disease. The long-term use of l-dopa therapy frequently leads to l-dopa-induced dyskinesia and several other non-motor flocculants for fluctuating plasma concentration. To alleviate the adverse effect of dopamine replacement therapy, sensitive detection of l-dopa is still challenging. Therefore, this study presents a simple strategy to develop an inexpensive, easy-to-use, smartphone-based fluorescence turn-on sensory system based on the aggregation-induced emission enhancement phenomenon for instant low-level detection of l-dopa in biological samples. The sensor was fabricated using silk-fibroin-decorated reduced graphene oxide quantum dots, which exhibit a progressive increase of fluorescence intensity in the presence of l-dopa. By measuring the turn-on efficiency of the sensor in elevated concentrations of l-dopa, the limit of detection (LOD) was evaluated as 76.18 nM over a linear range of 0–35 μM. We have further designed a smartphone-based electronic device involving a 365 nm light emitting diode attached below the rare camera for capturing the change of fluorescent color of the solution during sensing. From these images, red, green, and blue values are analyzed using a mobile phone application, and the corresponding LOD was found to be 0.29 μM in a similar linear range of concentration. This simple, cost-effective, and rapid screening gadget is very demanding for on-spot detection of the analytes in remote areas where sophisticated instrumentation is not usually available.

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“…19 It is well known that fluorescence turn-on-based sensing is superior to that of fluorescence turn-off-based sensing, which is attributed to a higher degree of selectivity, resolution, and lower potential errors for the former. 20 Also, fluorescence measurement-based sensing is straightforward and cost effective compared to other techniques such as high-performance liquid chromatography, 21 flow injection chemiluminescence, 22 radioimmunoassay, 23 micellar electrokinetic chroma-tography, 24 high-performance capillary electrophoresis, 25 etc. Therefore, the main goal of this work is to achieve fluorescence-based sensing of API and its pharmaceutical cocrystals using a fluorescence turn-on mechanism.…”

Section: ■ Introductionmentioning

confidence: 99%

Soot-Based Reduced Graphene Quantum Dot/Hemin Conjugate for Favipiravir Sensing

Hazarika

Kalita

Deka

et al. 2021

ACS Appl. Nano Mater.

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A conjugated system was synthesized from reduced graphene quantum dot (rGQD) and hemin for the selective detection of favipiravir (Fav), an antiviral drug that has come into much attention during the year 2020 for its use as a drug against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The required rGQD was prepared from soot particles using Hummers' method followed by the amino-hydrothermal process. At the first step, its fluorescence was quenched by preparing the conjugate with hemin. Interestingly, the fluorescence intensity gradually increases (turn-on) with increasing concentration of Fav, and develops 9-fold higher fluorescence at 15.6 nM of Fav. The fluorescence enhancement is selective, and the limit of detection (LOD) was calculated to be about 1.96 nM. The fluorescence turnon is governed by aggregation-induced emission (AIE), which originates from electrostatic interactions between the sensor−analyte systems. A similar fluorescence turn-on was observed for Fav in human blood plasma (BP) as well as in artificial urine (AU), which indicates that the sensor is viable in real-sample analysis. In addition to Fav, its 1:1 cocrystals with theophylline (Theo) and ferulic acid (FRA) also enhance the fluorescence in real samples with an LOD of 3.47 and 12.2 nM, respectively. Therefore, the cocrystals remain intact in biological medium and the sensor interacts with cocrystals too. The detection of Fav and its cocrystals, and the development of cocrystals as alternatives in the pharmaceutical industry, is essential considering the current COVID-19 pandemic worldwide. Therefore, the findings of this work will certainly help in developing fluorescence sensors for quantitative determination of active pharmaceutical ingredients (APIs) in real samples.

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A new approach for turn-on fluorescence sensing of l-DOPA

Cited by 22 publications

References 34 publications

Regulatory Activities of Dopamine and Its Derivatives toward Metal-Free and Metal-Induced Amyloid-β Aggregation, Oxidative Stress, and Inflammation in Alzheimer’s Disease

Regulatory Activities of Dopamine and Its Derivatives toward Metal-Free and Metal-Induced Amyloid-β Aggregation, Oxidative Stress, and Inflammation in Alzheimer’s Disease

Smart-Phone-Assisted Optical Biosensors Based on Silk-Fibroin-Decorated Reduced Graphene Oxide Quantum Dots for Fluorescent Turn-On Recognition of l-Dopa

Soot-Based Reduced Graphene Quantum Dot/Hemin Conjugate for Favipiravir Sensing

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