A New Approach for the Detection of Type III Hyperlipoproteinemia by RLP-Cholesterol Assay

Nakajima, Katsuyuki; Saito, Toshimitsu; Tamura, Aki; Suzuki, Manami; Nakano, Takamitsu; Adachi, Masakazu; Tanaka, Akira; Tada, Norio; Nakamura, Haruo; Murase, Toshio

doi:10.5551/jat1994.1.30

Cited by 48 publications

(29 citation statements)

References 18 publications

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“…RLP, not bound to the immunoaffinity chromatography that consists of JI-H and monoclonal antibody to apoA-l, contains chylomicron and a minor portion of VLDL. Utilization of this immunoaffinity mixed gel enables us to isolate RLP from plasma in a single process (9,(28)(29)(30)(31). We previously indicated that the magnitude of RLP-C and TG increments after oral fat load are greater in normolipidemic patients with angiologically defined CAD than in those without CAD (9).…”

Section: Discussionmentioning

confidence: 99%

Effects of Soy Protein Isolate (SPI) and Casein on the Postprandial Lipemia in Normolipidemic Men.

Shige

Ishikawa

Higashi

et al. 1998

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryTo elucidate the effects of soybean protein and casein on postprandial lipemia, oral fat load tests were performed before and 3 weeks after the administration of soy protein isolate (SPI) and casein supplement to normolipidemic men. Eleven normolipidemic male subjects on otherwise identical controlled diets were assigned to either a 20g/d soy protein isolate (SPI) dietary supplement or a casein dietary supple ment for three weeks in a crossover design. Fat load tests with 40g/m2 of bovine milk fat were carried out before and after 3weeks on the ex perimental dietary supplements. Fasting plasma concentrations of lipids and apolipoproteins were not significantly different from baseline levels before or after the administration of SPI or casein supplemented diets. Neither SPI nor casein supplement affected the fasting plasma con centrations of lipids and apolipoproteins. The areas under the incre mental curve (AUIC) of triglyceride (TG) and remnant-like particles triglyceride (RLP-TG) after both experimental diets were not significantly different from those before the experimental diets. However, the AUIC of remnant-like particles cholesterol (RLP-C) showed a tendency (p= 0.07) to decrease after administration of the diet supplemented with SPI than before the diet. The AUIC of RLP-C was significantly (p<0.05) lower after the diet supplemented with SPI than after administration of the diet supplemented with casein. These results suggest that 3weeks of 20g/d SPI dietary supplement favorably affects the postprandial remnant lipoprotein response as compared to the casein dietary supplement.

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Section: Discussionmentioning

confidence: 99%

Effects of Soy Protein Isolate (SPI) and Casein on the Postprandial Lipemia in Normolipidemic Men.

Shige

Ishikawa

Higashi

et al. 1998

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…The accuracy of this homogenous method has been described (79,80). RLP were quantified with an immunoseparation assay (81,82).…”

Section: Figurementioning

confidence: 99%

Consuming fructose-sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/obese humans

et al. 2009

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Studies in animals have documented that, compared with glucose, dietary fructose induces dyslipidemia and insulin resistance. To assess the relative effects of these dietary sugars during sustained consumption in humans, overweight and obese subjects consumed glucose-or fructose-sweetened beverages providing 25% of energy requirements for 10 weeks. Although both groups exhibited similar weight gain during the intervention, visceral adipose volume was significantly increased only in subjects consuming fructose. Fasting plasma triglyceride concentrations increased by approximately 10% during 10 weeks of glucose consumption but not after fructose consumption. In contrast, hepatic de novo lipogenesis (DNL) and the 23-hour postprandial triglyceride AUC were increased specifically during fructose consumption. Similarly, markers of altered lipid metabolism and lipoprotein remodeling, including fasting apoB, LDL, small dense LDL, oxidized LDL, and postprandial concentrations of remnant-like particle-triglyceride and -cholesterol significantly increased during fructose but not glucose consumption. In addition, fasting plasma glucose and insulin levels increased and insulin sensitivity decreased in subjects consuming fructose but not in those consuming glucose. These data suggest that dietary fructose specifically increases DNL, promotes dyslipidemia, decreases insulin sensitivity, and increases visceral adiposity in overweight/obese adults. IntroductionStudies investigating the effects of fructose consumption in humans and animals have been comprehensively reviewed (1-4), and while strong evidence exists that consumption of diets high in fructose results in increased de novo lipogenesis (DNL), dyslipidemia, insulin resistance, and obesity in animals, direct experimental evidence that consumption of fructose promotes DNL, dyslipidemia, insulin resistance, glucose intolerance, and obesity in humans is lacking. Thus, we have investigated and compared the biological effects of the 2 major simple sugars in the diet, glucose and fructose, on BW and regional fat deposition and on indices of lipid and carbohydrate metabolism in older, overweight and obese men and women.We sought to answer the following questions: (a) Does consumption of fructose with an ad libitum diet promote greater BW gain and have differential effects on regional adipose deposition and adipose gene expression compared with consumption of glucose with an ad libitum diet? (b) Does consumption of fructose induce dyslipidemia compared with consumption of glucose? (c) Is fructose-induced hypertriglyceridemia the result of increased rates

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“…However, the remnant lipoprotein was not clinically familiar, since there was no method for conveniently measuring the remnant lipoprotein. In 1993, we developed the remnant like particle (RLP) method for measuring serum remnant lipoproteins in clinical samples (3)(4)(5). As a result, clinical research revealed that RLP was easily taken into macrophages in arterial wall as well as oxidized LDL (6), promoted platelet aggregation (7), increased PAI-1 activity (8), impaired endothelial function (9), promoted proliferation of vascular smooth muscle cells (10,11), and promoted adhesion of monocytes to endothelial cells (12), showing atherogenic mechanism of RLP.…”

Section: Remnant Lipoproteins and Remnant Like Particles (Rlp)mentioning

confidence: 99%

Postprandial Hyperlipidemia and Atherosclerosis

Tanaka

2004

JAT

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The development of the remnant like particle (RLP) method for conveniently measuring serum remnant lipoprotein levels in 1993 promoted much research on atherogenic significance and metabolism of remnant lipoproteins. This research brought about many results as the following. A novel apolipoprotein B48 receptor incorporating remnant lipoproteins into macrophages in arterial wall was discovered and the structure of the gene of the receptor was clarified. The expression of apolipoprotein B100 was recognized in the human small intestine, suggesting that dietary very low density lipoproteins (VLDL) might be synthesized in the human small intestine and converted into VLDL remnants and low density lipoproteins (LDL). It is recognized that the atherosclerotic risk of postprandial hyperlipidemia is derived from an increase of remnant lipoproteins and that measurement of serum RLP levels in postprandial state is more sensitive and necessary for evaluating an atherosclerotic risk because serum RLP levels remain high all day in patients with diabetes mellitus or coronary heart disease. The relation between postprandial hyperlipidemia and insulin resistance was clarified. J Atheroscler Thromb, 2004; 11: 322-329.

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A New Approach for the Detection of Type III Hyperlipoproteinemia by RLP-Cholesterol Assay

Cited by 48 publications

References 18 publications

Effects of Soy Protein Isolate (SPI) and Casein on the Postprandial Lipemia in Normolipidemic Men.

Effects of Soy Protein Isolate (SPI) and Casein on the Postprandial Lipemia in Normolipidemic Men.

Consuming fructose-sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/obese humans

Postprandial Hyperlipidemia and Atherosclerosis

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