A new approach for the detection of cervical cancer in Thai women

Sindhuphak, Ratana; Issaravanich, Somchai; Udomprasertgul, Venus; Srisookho, Pailin; Warakamin, Suwanna; Sindhuphak, S; Boonbundarlchai, Rerngsak; Dusitsin, Nikorn

doi:10.1016/s0090-8258(03)00196-3

Cited by 45 publications

(35 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a dramatic decrease in the bands associated with glycogen (1036 cm -1 and 1152 cm -1 ). This reduction in glycogen has been observed in the literature 8,12,21,23 , and is to be expected due to the fact that glycogen levels increase in a cell as it ages. It is reasonable to assume that rapidly dividing cancer cells do not undergo the same ageing process as normal cells hence the glycogen differential.…”

Section: C-o-c C-o Dominated By the Ring Vibrations Of Carbohydratessupporting

confidence: 76%

“…It is reasonable to assume that rapidly dividing cancer cells do not undergo the same ageing process as normal cells hence the glycogen differential. Also, an increase in the nucleic acid band at 1236 cm -1 occurs with the onset of carcinogenesis, which too could be expected due to rapidly dividing cells and has been quoted in literature 7,23 . Wong et.…”

Section: C-o-c C-o Dominated By the Ring Vibrations Of Carbohydratesmentioning

confidence: 71%

See 1 more Smart Citation

The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

Faoláin¹,

Hunter

Byrne

et al. 2005

SPIE Proceedings

View full text Add to dashboard Cite

This work is licensed under a Creative Commons AttributionNoncommercial-Share Alike 3.0 License Recommended Citation OFaolain, E. et al. (2005) ABSTRACTThe application of vibrational spectroscopy to disease diagnosis is a relatively new, rapidly evolving scientific field. Techniques such as Raman and infrared spectroscopy have shown great promise in this regard over the past number of years. This study directly compared Raman spectroscopy and synchrotron infrared (SR-IR) spectroscopy on parallel cervical cancer samples. Both frozen and dewaxed formalin fixed paraffin preserved tissue sections were examined. Both tissue types produced good quality Raman and SR-IR spectra, although the lesser processed, frozen tissue sections displayed the most detailed spectra. Spectroscopy was shown capable of discriminating between different cell types in normal cervical tissue. Spectra recorded from invasive carcinoma showed a marked difference from those recorded from normal cervical epithelial cells. Spectral differences identified with the onset of carcinogenesis include increased nucleic acid contributions and decreased glycogen levels. These investigations pave the way for an enlarged study into this exciting new diagnostic field.

show abstract

Section: C-o-c C-o Dominated By the Ring Vibrations Of Carbohydratessupporting

confidence: 76%

Section: C-o-c C-o Dominated By the Ring Vibrations Of Carbohydratesmentioning

confidence: 71%

The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

Faoláin¹,

Hunter

Byrne

et al. 2005

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

“…Identifying the presence of HPV is a very important factor in the prevention of cervical cancer. Technologies such as 55 fluorescence or vibrational spectroscopies are being investigated as potential high sensitivity and specificity methodologies for cancer screening and diagnosis [8][9][10][11][12][13] . Vibrational spectroscopy is a molecular specific spectroscopy in which normal vibrational modes specific to molecular 60 bonds as well as more complex molecular fingerprints are probed.…”

Section: Introductionmentioning

confidence: 99%

“…The biochemical origins of the discrimination 50 can also be elucidated. The knowledge about differences in Raman and FTIR spectra between the HPV negative cells and these affected by various numbers of HPV copies is very important and has potential application in systems for cervical cancer screening based on vibrational spectroscopic 55 techniques. This new technology for cervical screening not only can distinguish cervical cells based on biochemical content but also can elucidate the effect of HPV on the cells.…”

mentioning

confidence: 99%

Investigation of the influence of high-risk human papillomavirus on the biochemical composition of cervical cancer cells using vibrational spectroscopy

Ostrowska¹,

Malkin²,

Meade³

et al. 2010

Analyst

View full text Add to dashboard Cite

The main aetiology of cervical cancer is infection with high risk human papillomavirus (HPV). Cervical cancer is almost 100% curable if detected in the early stages. Thus, information about the 10 presence and levels of HPV in patient samples has high clinical value. As current screening methods, such as the Pap smear test, are highly subjective and in many cases show low sensitivity and specificity, new supportive techniques are desirable to improve the quality of cervical cancer screening. In this study, vibrational spectroscopic techniques (Raman and Fourier Transform Infra Red absorption) have been applied to the investigation of four cervical cancer cell lines, HPV 15 negative C33A, HPV-18 positive HeLa with 20-50 integrated HPV copies per cell, HPV-16 positive SiHa with 1-2 integrated HPV strands per cell and HPV-16 positive CaSki containing 60-600 integrated HPV copies per cell. Results show that vibrational spectroscopic techniques can discriminate between the cell lines and elucidate cellular differences originating from proteins, nucleic acids and lipids. Similarities between C33A and SiHa cells were exhibited in the Raman 20 and infrared spectra and were confirmed by Principal Component Analysis (PCA). Analysis of the biochemical composition of the investigated cells, with the aid of PCA showed a clear discrimination between the C33A-SiHa group and HeLa and CaSki cell lines indicating the potential of vibrational spectroscopic techniques as support to current methods for cervical cancer screening. 25 IntroductionCervical cancer is second only to breast cancer as the most common malignancy in women worldwide 1 . The gradual progression of cervical cancer allows the application of screening and diagnostic programmes to detect neoplastic 30 changes before invasive cancer develops. The Papanicolaou test (also called the Pap test, Pap smear, or smear test) is a screening method which allows detection of infections and abnormalities of the cervix. The Pap smear test is the most widespread screening method for cervical neoplasia 2 and is 35 based on visual evaluation of the morphology of cells collected from the cervix under light microscopy. Microscopic examination brings subjectivity to the screening results, therefore, the smear test has a hugely variable sensitivity (17% -99%) and specificity (9% -100%) 3 . 40 The probability of developing cervical dysplasia is much higher for women infected with human papillomavirus (HPV) than those free of infection 4 . Infection with oncogenic or high-risk HPV is the main risk factor for cervical cancer 5 with 99.7% of invasive cervical neoplasia 45 associated with HPV infection 6 . Thus, HPV testing has been added to the range of clinical options for cervical cancer screening. Detection of HPV (HPV testing) is based on detecting the DNA of the virus. It has been found that the DNA of high risk strains of HPV are assimilated within the 50 cellular DNA of the host 7 . A drawback to HPV testing is that it is more expensive and time-consuming than other screening tes...

show abstract

“…Therefore, vibrational spectroscopy is becoming an increasingly powerful tool for the research on biochemistry of cancer [2][3][4][5] . Our research group has successfully used FT-IR spectroscopy to diagnose carcinomas, such as carcinoma of stomach, colon, esophagus, lung, salivary gland since 1995 [6][7][8][9] .…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Use of Fourier-transform infrared spectroscopy to rapidly diagnose gastric endoscopic biopsies

Sun²,

Xu³

et al. 2005

WJG

View full text Add to dashboard Cite

Abstract Abstract Abstract AIM:To determine if Fourier-transform infrared (FT-IR) spectroscopy of endoscopic biopsies could accurately diagnose gastritis and malignancy. METHODS:A total of 123 gastroscopic samples, including 11 cases of cancerous tissues, 63 cases of chronic atrophic gastritis tissues, 47 cases of chronic superficial gastritis tissues and 2 cases of normal tissues, were obtained from the First Hospital of Xi'an Jiaotong University, China. A modified attenuated total reflectance (ATR) accessory was linked to a WQD-500 FT-IR spectrometer for spectral measurement followed by submission of the samples for pathologic analysis. The spectral characteristics for different types of gastroscopic tissues were summarized and correlated with the corresponding pathologic results.RESULTS: Distinct differences were observed in the FT-IR spectra of normal, atrophic gastritis, superficial gastritis and malignant gastric tissues. The sensitivity of FT-IR for detection of gastric cancer, chronic atrophic gastritis and superficial gastritis was 90.9%, 82.5%, 91.5%, and specificity was 97.3%, 91.7%, 89.5% respectively. CONCLUSION:FT-IR spectroscopy can distinguish gastric inflammation from malignancy.

show abstract

A new approach for the detection of cervical cancer in Thai women

Cited by 45 publications

References 14 publications

The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

The potential of vibrational spectroscopy in the early detection of cervical cancer: an exciting emerging field

Investigation of the influence of high-risk human papillomavirus on the biochemical composition of cervical cancer cells using vibrational spectroscopy

Use of Fourier-transform infrared spectroscopy to rapidly diagnose gastric endoscopic biopsies

Contact Info

Product

Resources

About