A new approach for sustained and efficient H<sub>2</sub> photoproduction by <i>Chlamydomonas reinhardtii</i>

Kosourov, Sergey; Jokel, Martina; Aro, Eva‐Mari; Allahverdiyeva, Yagut

doi:10.1039/c8ee00054a

Cited by 80 publications

(92 citation statements)

References 30 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, bypassing the electron competition is the preferred path for engineering a continuous process of hydrogen production. Indeed, recent reports show that blocking the CBB cycle either by substrate limitation (Nagy et al, 2018) or avoiding its activation (Kosourov et al, 2018) supports continuous hydrogen production. In addition, preliminary engineering attempts (Nielsen et al, 2013;Wittenberg et al, 2013;Rumpel et al, 2014;Eilenberg et al, 2016;Mellor et al, 2016) provide promising results and in our opinion, should be incorporated into a "super mosaic" single algae clone.…”

Section: Resultsmentioning

confidence: 99%

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

et al. 2018

View full text Add to dashboard Cite

Photoproduction of hydrogen by green algae is considered a transitory release valve of excess reducing power and a potential carbon-free source of sustainable energy. It is generally accepted that the transitory production of hydrogen is governed by fast inactivation of hydrogenase by oxygen. However, our data suggest that photosynthetic electron loss to competing processes, mainly carbon fixation, stops hydrogen production, supports hydrogen uptake, and precedes the inevitable inactivation by oxygen. Here, we show that when transitioning from dark anaerobiosis to light, hydrogen production ceases within 2 min, regardless of the presence of oxygen. Simultaneous monitoring of the active hydrogenase pool size shows that it remains entirely intact up to 4 min after illumination and is inactivated only later. Thus, our data reveal a window of 4 min in which the hydrogenase pool is not being degraded by oxygen. Furthermore, we show that electron loss, prominently to carbon fixation, outcompetes hydrogen production and leads to hydrogen uptake. Indeed, supplying additional reducing power to hydrogenase at the cessation point regenerates the accumulation of hydrogen. Our results imply the fast cessation of hydrogen production is governed by electron loss rather than oxygen inactivation, which takes place minutes later.

show abstract

Section: Resultsmentioning

confidence: 99%

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

et al. 2018

View full text Add to dashboard Cite

show abstract

“…No direct formation of H 2 O 2 from H 2 and O 2 is expected in aerobic cells because the production of hydrogen requires anaerobic conditions [77]. H 2 is consumed by the bidirectional hydrogenase in green algae [78], but an enzyme-catalyzing Reaction (52) has not been found.…”

Section: Formation Of H2o2mentioning

confidence: 99%

Oxygen and ROS in Photosynthesis

Khorobrykh

Havurinne

Mattila

et al. 2020

Plants

186

133

View full text Add to dashboard Cite

Oxygen is a natural acceptor of electrons in the respiratory pathway of aerobic organisms and in many other biochemical reactions. Aerobic metabolism is always associated with the formation of reactive oxygen species (ROS). ROS may damage biomolecules but are also involved in regulatory functions of photosynthetic organisms. This review presents the main properties of ROS, the formation of ROS in the photosynthetic electron transport chain and in the stroma of chloroplasts, and ROS scavenging systems of thylakoid membrane and stroma. Effects of ROS on the photosynthetic apparatus and their roles in redox signaling are discussed.

show abstract

“…The use of MIMS and of various C. reinhardtii mutants allowed identifying biological bottlenecks limiting the supply of electrons to the H 2 ase (Toth and Yacoby, 2019;Burlacot et al, 2020a). Lately, MIMS was used in the development of a very promising H 2 photoproducing protocol using flashing light as the light source in photoautotrophic selfanaerobic conditions (Kosourov et al, 2018). MIMS has early been used for in vitro and in vivo measurements of H 2 ase activity (Jouanneau et al, 1980;Vignais et al, 1982;Berlier et al, 1985).…”

Section: Hydrogen Production and Hydrogenase Activity Measurementsmentioning

confidence: 99%

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Burlacot

Li‐Beisson

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Since the first great oxygenation event, photosynthetic microorganisms have continuously shaped the Earth's atmosphere. Studying biological mechanisms involved in the interaction between microalgae and cyanobacteria with the Earth's atmosphere requires the monitoring of gas exchange. Membrane inlet mass spectrometry (MIMS) has been developed in the early 1960s to study gas exchange mechanisms of photosynthetic cells. It has since played an important role in investigating various cellular processes that involve gaseous compounds (O 2 , CO 2 , NO, or H 2) and in characterizing enzymatic activities in vitro or in vivo. With the development of affordable mass spectrometers, MIMS is gaining wide popularity and is now used by an increasing number of laboratories. However, it still requires an important theory and practical considerations to be used. Here, we provide a practical guide describing the current technical basis of a MIMS setup and the general principles of data processing. We further review how MIMS can be used to study various aspects of algal research and discuss how MIMS will be useful in addressing future scientific challenges.

show abstract

A new approach for sustained and efficient H₂ photoproduction by Chlamydomonas reinhardtii

Abstract: Pulse-illumination re-directs photosynthetic electron flow destined for CO2 fixation to [FeFe]-hydrogenase, thus sustaining algal H2 photoproduction for 70 hours.

Cited by 80 publications

References 30 publications

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

Oxygen and ROS in Photosynthesis

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Contact Info

Product

Resources

About

A new approach for sustained and efficient H2 photoproduction by Chlamydomonas reinhardtii

Abstract: Pulse-illumination re-directs photosynthetic electron flow destined for CO2 fixation to [FeFe]-hydrogenase, thus sustaining algal H2 photoproduction for 70 hours.

Cited by 80 publications

References 30 publications

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place

Oxygen and ROS in Photosynthesis

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Contact Info

Product

Resources

About

A new approach for sustained and efficient H₂ photoproduction by Chlamydomonas reinhardtii