A New Approach for Fabricating Collagen/ECM‐Based Bioinks Using Preosteoblasts and Human Adipose Stem Cells

Abstract

Cell-printing methods have been used widely in tissue regeneration because they enable fabricating biomimetic 3D structures laden with various cells. To achieve a cell-matrix block, various natural hydrogels that are nontoxic, biocompatible, and printable have been combined to obtain "bioinks." Unfortunately, most bioinks, including those with alginates, show low cell-activating properties. Here, a strategy for obtaining highly bioactive ink, which consisted of collagen/extracellular matrix (ECM) and alginate, for printing 3D porous cell blocks is developed. An in vitro assessment of the 3D porous structures laden with preosteoblasts and human adipose stem cells (hASCs) demonstrates that the cells in the bioinks are viable. Osteogenic activities with the designed bioinks show much higher levels than with the "conventional" alginate-based bioink. Furthermore, the hepatogenic differentiation ability of hASCs with the bioink is evaluated using the liver-specific genes, albumin, and TDO2, under hepatogenic differentiation conditions. The genes are activated within the 3D cell block fabricated using the new bioink. These results demonstrate that the 3D cell-laden structure fabricated using collagen/ECM-based bioinks can provide a novel platform for various tissue engineering applications.

Three-Dimensional Bioprinting of Polycaprolactone Reinforced Gene Activated Bioinks for Bone Tissue Engineering

“…The choice of material for the gene activated bioink was motivated by a number of factors, including the printability of the alginate hydrogel, the presence of the RGD ligand to allow cell spreading, the ability to facilitate calcium phosphate based gene delivery and established capacity to enable the osteogenic differentiation of MSCs (10, 19,23,[31][32][33][34]. Polymeric scaffolds are typically inert and may require supplementation with various factors in order to induce a favourable biological response, often provided through the addition of extracellular matrix components, or exogenous growth factors (35)(36)(37)(38). A number of publications have also reported superior biological activity solely due to the addition of alginate hydrogel to PCL scaffolds (39,40).…”

Large‐scale high‐density culture of hepatocytes in a liver microsystem with mimicked sinusoid blood flow

“…However, promising performance can still be expected. In addition, for the microsystem designed here, a better outcome can be achieved by means of coculturing multiple cells (Du et al, ), adjusting the stiffness of the alginate ECM (Weng et al, ) or collagen ECM (Lee et al, ), and adding hepatocyte growth factor to the culture medium (Huh et al, ).…”

Tissue Engineering in Liver Regenerative Medicine: Insights into Novel Translational Technologies

“…The synthetic bio-inks may include: polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), pluronic polymers [67,68], etc. The ideal bi-oink should have the proper physiochemical properties, such as suitable mechanical, rheological, chemical and biological ones [69]. A practical biomaterial for 3D bio-printing is usually a biocompatible substance, which should be easily manipulated and it could maintain or even enhance cell viability and functions [70].…”