A new application of inorganic sorbent for biomolecules: IMAC practice of Fe3+-nano flowers for DNA separation

Immobilization of Alpha‐Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater

“…[7] Affinity chromatography techniques take place wide implementations among all classic purification methods due to less purification steps. [8][9][10] Lately, immobilized metal affinity chromatography (IMAC) has been generally selected for the purification of biomolecules because of its excellent features such as low cost, high biomolecule adsorption capability, and great chemical stability. These characteristics are very important for large-ratio purification in industrial practises.…”

“…[ 12 ] Metal ion immobilization on some adsorbents may enhance the interaction of them with biomolecules, which is desired for applications in IMAC. [ 9 ] It has been reported in the literature that glutamate and aspartate also contribute to the binding, as well as proteins with amino acids that can be widely used in the IMAC process and can be reached on the surface. Even though the IMAC technique is active with His and/or Cys rich proteins in a certain pH range (6.0–8.0), the presence of amino acids such as glutamate and aspartate also offers the opportunity for IMAC to work below these pHs.…”

Generation of zinc ion-rich surface via in situ growth of ZIF-8 particle: Microorganism immobilization onto fabric surface for prohibit hospital-acquired infection

“…Copper phosphate nanoflowers have been used to improve DNA adsorption efficiency. It was reported that the maximum absorption capacity rose from 25.3 mg/g to 845.8 mg/g due to the addition of Fe(III) ion [15] . Influenza virus membrane protein (M2e) can stably conjugate with gold nps via the thiol-gold interaction from the cysteine residue and resulting in the 29% improvement of immobilization efficiency [16] .…”

Investigation of BSA adsorption performances of metal ion attached mineral particles embedded cryogel discs

“…Immobilized metal affinity chromatography (IMAC) presented by Porath et al (1975) has turned into a common analytic and preparative separation technique on purification of some biomolecules [9,10,11,12,13]. Proteins with standard of high purity, which are extensively utilized in various fields, such as molecular biology and biotechnology, can be gained through His-tagged recombinant proteins which require IMAC [14,10].…”