A new apparatus to measure rate of desquamation using dansyl chloride fluorescence

Takahashi, Masanori; Machida, Yasuhiko; Marks, R.

doi:10.1007/bf00417331

Cited by 11 publications

(7 citation statements)

References 4 publications

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“…These findings are characteristic of all chronic inflammatory dermatoses. 12 However, in AX we were unable to show an increase in parakeratotic cells. Evidently, the underlying inflammation is too mild.…”

Section: Commentcontrasting

confidence: 58%

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Functional Analyses of the Superficial Stratum Corneum in Atopic Xerosis

Watanabe

Tagami²,

Horii³

et al. 1991

Arch Dermatol

146

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Dermatologists universally recognize that the unaffected skin of patients with atopic dermatitis tends to be dry and slightly scaly. To characterize the functional properties of the superficial stratum corneum in atopic xerosis, we studied the forearms of 28 patients with atopic dermatitis, aged 14 to 30 years, and 18 age-matched controls, with the use of mainly noninvasive methods. Patients with atopic xerosis showed markedly higher transepidermal water loss and markedly lower skin surface hydration levels than did the controls. The corneocytes in atopic xerosis tended to desquamate in clumps of cell aggregates instead of as individual cells. They contained a substantially lower amount of water-soluble amino acids, which play a role in the water-retaining capacity of stratum corneum, than did those of controls. Although the number of stratum corneum cell layers in atopic xerosis (21 +/- 4) was substantially larger than that in controls (15 +/- 1), its turnover time (7 +/- 2 days) was appreciably shorter than that for controls (14 +/- 2 days). Like those noted in the skin with increased epidermal proliferation, the size of superficial corneocytes in patients with atopic xerosis was substantially smaller than in controls. Histopathologic examination revealed acanthotic epidermis, mild perivascular mononuclear cell infiltrate, and pigment incontinence. Atopic xerosis, the dry skin of patients with atopic dermatitis, shows various stratum corneum functional impairments, probably reflecting increased epidermal proliferation due to a low-level ongoing dermatitis.

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Section: Commentcontrasting

confidence: 58%

“…Turnover time of the SC was measured with the dansyl chloride method as modified by Takahashi et al 12 Briefly, a 5% dansyl chloride in white soft paraffin was applied to the skin under occlusion for 24 hours. This resulted in staining of the entire thickness of the SC.…”

Section: Noninvasive Measurements Of the Scmentioning

confidence: 99%

Functional Analyses of the Superficial Stratum Corneum in Atopic Xerosis

Watanabe

Tagami²,

Horii³

et al. 1991

Arch Dermatol

146

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“…The fluorescent stain dansyl chloride (DC) has been widely used as a marker to assess stratum corneum turnover time and exfoliation rate. These parameters are currently determined by measuring the decay in DC fluorescence from stained skin (4)(5)(6)(7)(8). However, potential issues, both experimental and clinical, exist with the use of DC.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo¶

Forest

Grothaus

Ertel

et al. 2003

Photochem Photobiol

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Dihydroxyacetone (DHA) has been proposed as a potential alternative to dansyl chloride for use as a fluorescence marker on skin to assess stratum corneum turnover time in vivo. However, the fluorescence from DHA on skin has not been adequately studied. To address this void, a noninvasive, noncontact spectral imaging system is used to characterize the fluorescence spectrum of DHA on skin in vivo and to determine the optimal wavelengths over which to collect the DHA signal that minimizes the contributions from skin autofluorescence. The DHA-skin fluorescence signal dominates the 580-680 nm region of the visible spectrum when excited with ultraviolet radiation in the 320-400 nm wavelength region (UVA). An explanation of the time-dependent spectral features is proposed in terms of DHA polymerization and binding to skin.

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“…The data measured in the 7 patients exhib¬ ited a pattern of an initial high level that gradually de¬ scended to the level of the controls (Figure 1, bottom). However, these were too varied to show any significant differences in comparison with the controls except for the early stage between 15 and 49 days after the grafts were harvested. In view of these findings, we concluded that the TEWL value was a more suitable parameter for determining normalization of the SC at scar sites.…”

Section: Scars At Donor Sites Of Split-thickness Skin Graftsmentioning

confidence: 90%

Functional Analyses of the Stratum Corneum in Scars

Suetake¹

1996

Arch Dermatol

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A new apparatus to measure rate of desquamation using dansyl chloride fluorescence

Cited by 11 publications

References 4 publications

Functional Analyses of the Superficial Stratum Corneum in Atopic Xerosis

Functional Analyses of the Superficial Stratum Corneum in Atopic Xerosis

Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo¶

Functional Analyses of the Stratum Corneum in Scars

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