A new antimicrobial peptide SCY2 identified in Scylla Paramamosain exerting a potential role of reproductive immunity

Qiao, Kun; Xu, Wan-Fang; Chen, Huiyun; Zhang, Yaqun; Huang, Wei‐Jan; Wang, Shuping; An, Zhe; Shan, Zhongguo; Chen, Fang-Yi; Wang, Kejian

doi:10.1016/j.fsi.2016.02.022

Cited by 22 publications

(34 citation statements)

References 48 publications

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“…and gonads (scygonadin, SCY2, etc.) of the mud crab (Huang et al, 2006;Qiao et al, 2016;Shan et al, 2016), and the present study is a continuation of our ongoing works on characterizing new AMPs from mud crab. In this study, a novel AMP, scyreprocin, was identified as an interacting partner of SCY2 from the reproductive system of male S. paramamosain.…”

Section: Discussionmentioning

confidence: 65%

“…Synthesis of selected antigen sites (CSGNKGKESKDPKVK), preparation, and quality inspection of the antibody were performed by GenScript (NJ, CHN). SCY2 antibody was prepared as previously described (Qiao et al, 2016). The specificity of SCY2 and scyreprocin antibodies were analyzed following the standard Western blotting procedure.…”

Section: Polyclonal Antibody Preparationmentioning

confidence: 99%

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A Novel Antimicrobial Peptide Scyreprocin From Mud Crab Scylla paramamosain Showing Potent Antifungal and Anti-biofilm Activity

Yang

Chen

et al. 2020

Front. Microbiol.

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Natural antimicrobial peptides (AMPs) are potential antibiotic alternatives. Marine crustaceans are thought to generate more powerful and various AMPs to protect themselves from infections caused by pathogenic microorganisms in their complex aquatic habitat, thus becoming one of the most promising sources of AMPs or other bioactive substances. In the study, a novel protein was identified as an interacting partner of male-specific AMP SCY2 in Scylla paramamosain and named scyreprocin. The recombinant product of scyreprocin (rScyreprocin) was successfully expressed in Escherichia coli. rScyreprocin exerted potent, broad-spectrum antifungal, antibacterial, and anti-biofilm activity (minimum inhibitory concentrations from 0.5 to 32 µM) through differential modes of action, including disruption of cell membrane integrity and induction of cell apoptosis, and has rapid bactericidal (in 0.5-2 h) and fungicidal (in 8-10 h) kinetics. In addition to its fungicidal activity against planktonic fungi, rScyreprocin also prevented the adhesion of fungal cells, inhibited biofilm formation, and eradicated the mature biofilms. Moreover, rScyreprocin showed a profound inhibitory effect on spore germination of Aspergillus spp. (minimum inhibitory concentrations from 4 to 8 µM). This peptide was not cytotoxic to murine and mammalian cells and could increase the survival rate of Oryzias melastigma under the challenge of Vibrio harveyi. Taken together, the novel AMP scyreprocin would be a promising alternative to antibiotics used in aquaculture and medicine.

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Section: Discussionmentioning

confidence: 65%

Section: Polyclonal Antibody Preparationmentioning

confidence: 99%

A Novel Antimicrobial Peptide Scyreprocin From Mud Crab Scylla paramamosain Showing Potent Antifungal and Anti-biofilm Activity

Yang

Chen

et al. 2020

Front. Microbiol.

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“…This would be important as mating occurs in crabs when the female has moulted and is susceptible to microbial incursion through the soft, uncalcified epidermis. Certainly, several reports already exist that antimicrobial proteins are associated with the reproductive organs of crabs (Jayasankar and Subramoniam, 1999;Huang et al, 2006;Wang et al, 2007;Qiao et al, 2016;Xu et al, 2011a, b) as well as in some other arthropods (Samakovlis et al, 1991). Indeed there are some WFDSC-domain containing proteins, particularly eppin, that occur in the testes and/or ovaries of mammals (Yenugu et al, 2004;Trexler et al, 2002; see also review by Bingle and Vyacarnam, 2008).…”

Section: Discussionmentioning

confidence: 99%

Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas, reveals its multi-functionality

Suleiman

Smith

Dyrynda

2017

Developmental & Comparative Immunology

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Crustins are whey acidic four-disulphide core (WFDSC) domain-containing proteins in decapods that are widely regarded as antimicrobial agents that contribute to host defence. Whilst there have been many analyses of crustin gene expression in tissues, few studies have been made of the distribution of the natural proteins. Here we report an immunostaining investigation of carcinin, a native crustin from Carcinus maenas, in the body organs. The results show that the protein is largely confined to the haemocytes with only a weak signal detected in the heart, hepatopancreas and midgut caecum where it is restricted to the outer surfaces. Importantly, carcinin was seen to be deposited by the haemocytes on these surfaces. Higher levels of staining were detected in the gonads with carcinin particularly abundant in the capsule of ovary as well as some oocytes. Conspicuous staining was further evident in the cuticle of the eyestalk peduncles. Ablation of the eyestalks resulted in a reduction of carcinin in the maturing ovary with the mature eggs rarely displaying a strong signal for the protein. Interestingly, the degree of carcinin also strongly increased in the healing peduncle, indicating that the protein may be associated with wounding, cell damage and/or tissue regeneration.

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“…In this case, the histone derived peptide was designed as sphistin in order to function as AMP molecules when the antimicrobial activity is exerted against aquatic pathogens such as Aeromonas hydrophila, Pseudomonas fluorescens, and Pseudomonas stutzeri. AMP also plays a role in reproductive immune function of mud crabs as described by Qiao et al [44], whereby a new AMP known as SCY2 was found which is dominantly expressed in the ejaculatory duct of male crabs. Amino acid sequences of SCY2 were 65.08% similar to the previous scygonadin found in seminal plasma of S. serrata.…”

Section: Antimicrobial Peptides In Mud Crabsmentioning

confidence: 94%

A Brief Review on the Antioxidants and Antimicrobial Peptides Revealed in Mud Crabs from the Genus of Scylla

Yusof

Ahmad

Swamy

2017

Journal of Marine Biology

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Mud crab from the genus Scylla is also known as mangrove crab, which has been well-accepted as a good source of protein.Recently, the antioxidant properties present in mud crabs have been reported to have a part in the protection of cells against free radicals. Meanwhile, numerous antimicrobial peptides from mud crabs have managed to be characterized through the display of antimicrobial activities against Gram-positive and Gram-negative bacteria. Hence, this paper is an effort to collect recent literatures on antioxidant and antimicrobial properties in every part of mud crabs which include muscle tissue, hemolymph, and crab shell. Moreover, the effort to understand the biological properties of mud crabs is important to enhance its production in aquaculture industry. Therefore, this review hoped to attract the attention of natural product researchers to focus on the potential therapeutic applications of mud crabs.

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A new antimicrobial peptide SCY2 identified in Scylla Paramamosain exerting a potential role of reproductive immunity

Cited by 22 publications

References 48 publications

A Novel Antimicrobial Peptide Scyreprocin From Mud Crab Scylla paramamosain Showing Potent Antifungal and Anti-biofilm Activity

A Novel Antimicrobial Peptide Scyreprocin From Mud Crab Scylla paramamosain Showing Potent Antifungal and Anti-biofilm Activity

Unusual tissue distribution of carcinin, an antibacterial crustin, in the crab, Carcinus maenas, reveals its multi-functionality

A Brief Review on the Antioxidants and Antimicrobial Peptides Revealed in Mud Crabs from the Genus of Scylla

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