A New Antimicrobial Agent: Poly (3‐hydroxybutyric acid) Oligomer

Ma, Linlin; Zhang, Ziheng; Li, Jun; Yang, Xingxing; Fei, Bin; Leung, Polly H.M.; Tao, Xiaoming

doi:10.1002/mabi.201800432

Cited by 31 publications

(25 citation statements)

References 72 publications

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“…HCl solution (2 m, 20 mL) was injected to quench the reaction, prior to an extraction with DCM. PHB oligomer was obtained after removal of the DCM by vacuum evaporation and separation by column chromatography (DCM/n-hexane = 5:1, v: v) [9].…”

Section: Preparation Of Phb Oligomer By Chemical Synthesismentioning

confidence: 99%

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Mechanistic Study of Synergistic Antimicrobial Effects between Poly (3-hydroxybutyrate) Oligomer and Polyethylene Glycol

Zhang

et al. 2020

Preprint

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We reported previously that poly (3-hydroxybutyrate) (PHB) oligomer is an effective antimicrobial agent against gram-positive bacteria, gram-negative bacteria, fungi and multi-drug resistant bacteria. In this work, it was further found that polyethylene glycol (PEG) can promote the antimicrobial effect of PHB oligomer synergistically. Three hypothetic mechanisms were proposed, that is, generation of new antimicrobial components, degradation of PHB macromolecules and dissolution/dispersion of PHB oligomer by PEG. With a series of systematic experiments and characterizations of HPLC-MS, it was deducted that dissolution/dispersion of PHB oligomer dominated the synergistic antimicrobial effect between PHB oligomer and PEG. This work demonstrates a way for promoting antimicrobial effect of PHB oligomer and other antimicrobial agents through improving hydrophilicity.

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Section: Preparation Of Phb Oligomer By Chemical Synthesismentioning

confidence: 99%

“…We have reported previously that the synthesized poly (3- (methicillin-resistant S. aureus, ATCC 43300) with reduction rate of 99.97% [9]. The PHB oligomer is degradable, durable, eco-friendly and safe, thus has significant advantages for the applications in healthcare field.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Study of Synergistic Antimicrobial Effects between Poly (3-hydroxybutyrate) Oligomer and Polyethylene Glycol

Zhang

et al. 2020

Preprint

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“…In the present study, we attempted to perform the secretory production of 3HB oligomers (3HBOs), which are widely distributed as physiologically active substances in nature and are found in various prokaryotes and eukaryotes . For example, 3HBOs have been identified as a sex pheromone in a spider and as a growth‐promoting factor in Frateuria bacterium in relation to soil bacteria diversification .…”

Section: Introductionmentioning

confidence: 99%

“…For example, 3HBOs have been identified as a sex pheromone in a spider and as a growth‐promoting factor in Frateuria bacterium in relation to soil bacteria diversification . Most recently, 3HBOs were also reported to exhibit antibacterial activity against some pathogenic and drug‐resistant bacteria . Terminally methyl‐esterified 3HBOs are known to effectively scavenge hydroxyl radicals .…”

Section: Introductionmentioning

confidence: 99%

Microbial Secretion Platform for 3‐Hydroxybutyrate Oligomer and Its End‐Capped Forms Using Chain Transfer Reaction‐Mediated Polyhydroxyalkanoate Synthases

Miyahara

Hiroe

Tsuge

et al. 2019

Biotechnology Journal

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The biodegradable polyester 3‐hydroxybutyrate (3HB) polymer [P(3HB)] is intracellularly synthesized and accumulated in recombinant Escherichia coli. In this study, native polyhydroxyalkanoate (PHA) synthases are used to attempt to microbially secrete 3HB homo‐oligomers (3HBOs), which are widely distributed in nature as physiologically active substances. High secretory production is observed, especially for the two PHA synthases from Aeromonas caviae and Bacillus cereus YB4. Surprisingly, an ethyl ester at the carboxy terminus (ethyl ester form) of 3HBOs is identified for most of the PHA synthases tested. Next, 3HBOs with a functional carboxyl group (carboxyl form of 3HBO) are obtained by using the alcohol dehydrogenase gene (adhE)‐deficient mutant strain, suggesting that the endogenous ethanol produced in E. coli acts as a chain transfer (CT) agent in the generation of 3HBOs. Furthermore, an in vitro polymerization assay reveals that CT agents such as ethanol and free 3HB are involved in the generation of ethyl ester and carboxyl form of 3HBO, respectively. The microbial platform established herein allows the secretion of 3HBOs with desirable end structures by supplementation with various CT agents. The obtained 3HBOs and their end‐capped forms may be used as physiologically active substances and building blocks for polymeric materials.

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“…The synthesis of polymers in plants is an interesting issue and it can be mainly performed not for the production of these compounds, but rather to change plant properties. Recently it has been shown that PHB oligomers may have antifungal and antibacterial properties (Ma et al 2019), and that amino-PHB polymers may acts as anti-cancer and antimicrobial agents (Abdelwahab et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

Wróbel‐Kwiatkowska

Kostyń

Dymińska

et al. 2020

Plant Cell Tiss Organ Cult

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Linum usitatissimum L. is a plant used by human since ancient times. Presently flax has both industrial and nutritional significance. The programmes of restoration of flax cultivation and processing are implemented to renew the importance of this plant for agriculture and economy. Genetic engineering methods and techniques of plant tissue cultures can answer this requirement, because they give the opportunity for obtaining of new plants and novel application of them. In the present study the impact of genetic modification of flax plants on the ability of morphogenesis was investigated. The subject of this research remain genetically modified flax plants enriched in poly-β-hydroxybutyrate (PHB), interesting biodegradable compound showing bioactive properties and acting as anti-inflammatory and anti-microbial factor. Another aim of this study was to characterize chemical and structural properties of callus derived from the engineered plants in comparison to the data obtained for non-transgenic, control callus cultures. The presence of PHB was confirmed by FTIR technique and it was suggested that this polymer may have impact on about 20% higher biomass growth observed for transgenic callus cultures in comparison to control cultures. The level of phenolic compounds was not significantly changed in the transgenic calluses when compared to wild-type cultures. The extracellular matrix (EMC) was noticed for the surface of genetically modified calluses, such structure was not visible for calluses derived from the control cultures. The cellulose in plant cell walls of transgenic calluses was structurally changed, it was not as organized as in control callus, resulting in lower crystallinity index values. Key message 1. FTIR and SEM analyses revealed differences between callus producing PHB when compared to wild-type callus. 2. PHB might influence the proliferative properties of plant cells.

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A New Antimicrobial Agent: Poly (3‐hydroxybutyric acid) Oligomer

Cited by 31 publications

References 72 publications

Mechanistic Study of Synergistic Antimicrobial Effects between Poly (3-hydroxybutyrate) Oligomer and Polyethylene Glycol

Mechanistic Study of Synergistic Antimicrobial Effects between Poly (3-hydroxybutyrate) Oligomer and Polyethylene Glycol

Microbial Secretion Platform for 3‐Hydroxybutyrate Oligomer and Its End‐Capped Forms Using Chain Transfer Reaction‐Mediated Polyhydroxyalkanoate Synthases

Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

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