A new antibacterial lupane ester from the seeds of<i>Acokanthera oppositifolia</i>Lam.

Sayed, Abeer M. El; Ezzat, Shahira M.; Sabry, Omar M.

doi:10.1080/14786419.2016.1166494

Cited by 17 publications

(8 citation statements)

References 19 publications

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“…1 NMR (400 MHz in CDCl 3 ) and 13 CNMR (100 MHz, in CDCl 3 ) chemical shift assignments for AcoA were reported only recently (El Sayed et al, 2016). The structure of the isolated compound is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice

Ezzat

Gaafary

Sayed

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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The current study aimed to investigate the protective effect of the cardenolide glycoside acovenoside A (AcoA) against doxorubicininduced cardiotoxicity in mice. AcoA was isolated from the pericarps of Acokanthera oppositifolia to chemical homogeneity and characterized by means of one-and two-dimensional nuclear magnetic resonance spectroscopy. AcoA exhibited relatively low toxicity in mice (LD 50 5 223.3 mg/kg bw). Repeated administration of doxorubicin induced cardiotoxicity manifested by reduced activity of myocardial membrane-bound ion pumps and elevated serum biomarkers of myocardial dysfunction, oxidative stress, and inflammation. Pretreatment of doxorubicin-exposed mice with AcoA (11.16 or 22.33 mg/kg bw, i.p.) for 2 weeks after 2 weeks of combined administration of AcoA and doxorubicin protected the animals dose dependently against doxorubicininduced cardiotoxicity as indicated by normalization of the levels of different myocardial markers of oxidative stress (malondialdehyde, nitric oxide, total antioxidant capacity, and cardiac glutathione), serum myocardial diagnostic marker enzymes (serum cardiac troponin T, creatine kinase isoenzyme MB, aspartate aminotransferase, and lactate dehydrogenase), and inflammatory markers (c-reactive protein, tumor necrosis factor-a, and interleukin-6), as well as myocardial Na 1 /K 1 -ATPase activity. These effects were attributed to the negative impact of AcoA on transcription factors nuclear factor kB and interferon regulatory factor 3/7. Thus acovenoside A might act as a cardioprotective agent to prevent doxorubicin-induced cardiotoxicity.

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Section: Resultsmentioning

confidence: 99%

“…Recently, it was shown that the cardenolide glycoside acovenoside A (AcoA) present in Acokanthera oppositifolia in small quantities, inhibits the growth of Escherichia coli (El Sayed et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice

Ezzat

Gaafary

Sayed

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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“…The literature provides controversial data regarding the antibacterial activity of lupeol. While some authors report the activity of this compound against Gram-positive bacteria and fungi [ 39 ], others report that the antibacterial activity of isolated triterpenes, including lupeol, expressed as MIC, is higher than 1.0 mg/mL [ 40 ]. Triterpenes were also identified in fraction Hex/CHCL 3 , as some derivatives of amyrin, sitosterol, and stigmasterol.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of virulent and resistant Escherichia coli strains to non-polar and polar compounds identified in Microplumeria anomala

et al. 2020

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Background and Aim: Escherichia coli is one of the main pathogens responsible for veterinary and human infections, and it is associated with significant economic losses in the livestock, as it causes severe diseases to humans, particularly in children. For that reason, there is a need for introducing new drugs to treat E. coli diseases. The Brazilian species richness is a source of potential new antibacterial natural products. The study aimed at the biological and chemical investigation of the organic extract obtained from the stem of Microplumeria anomala (Apocynaceae), EB127, as it was identified as a potential source of new antibacterial compounds to be used in Veterinary. Materials and Methods: The antibacterial activity was evaluated by disk diffusion and microdilution assays; chromatography, nuclear magnetic resonance spectrometry, and mass spectrometry were used in the isolation and identification of compounds. Results: EB127 showed activity against E. coli ATCC25922, and against three E. coli strains that were isolated from frigarte's cloaca, named 31/1A, 35A, and 51A. Lupeol, 3-acetyl-11-oxo-β-amyrin, 3-acetyl-11-oxo-α-amyrin, sitosterol, stigmasterol, 3β,7α-dihydroxy-cholest-5-ene, 3β-hydroxy-cholest-5-en-7-one, and 3β-hydroxy-cholest-5,22-dien-7-one were identified in fraction Hex/CHCl3, while loganin, loganic acid, methylanomaline, and anomaline were all identified in EB127 and protocatechuic acid hexoside, ferulic acid, secoxyloganin, feruloylquinic acid, vanillic acid hexoside, protocatechuic acid-4-O-β-hexoside, and rosmarinic acid were tentatively identified in fraction 10%ACN/H2O. E. coli 51A (virulent/non-resistant) showed sensitivity to the antibacterial action of fraction Hex/CHCl3 which contains alkaloids, triterpenes, and steroids, while E. coli 35A (resistant/non-virulent) were more susceptible to 10%ACN/H2O, which contains iridoids as loganin and loganic acid, and glycosylated and non-glycosylated caffeic acids. Conclusion: Fraction 10%ACN/H2O is of interest in pursuing new drugs to treat resistant E. coli, in veterinary. All compounds were isolated from the plant for the first time and have shown potential as new antibacterial natural products from Amazon plants to be used in veterinary and human diseases.

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“…As atividades biológicas desta planta podem ser inferidas pelas atividades próprias dos compostos isolados, assim o composto 1 é acaricida, 34 antimicrobiano, 35,36 anti-inflamatório e analgésico, 37 antimalárico 38 e tripanocida, 39 o 3 é antimicrobiano, 40 citotóxico, 41,42 analgésico e antiflamatório, 43 anti-HIV, 44 tripanocida, 45 entre outras; o composto 5 tem atividade citotóxica; 46 o composto 6 é antimicrobiano, 47 citotóxico, 48,49 entre outras; o composto 8 é citotóxico; 50 o 9 é antimicrobiano, 51 antidiabético e antioxidante. 52 Comparando os usos, pela medicina tradicional, das várias partes da C. gratissimus com as atividades dos compostos isolados verifica-se que a maior parte dos usos são compatíveis com os resultados deste estudo.…”

Section: Conclusõesunclassified