A new anti-infective strategy to reduce the spreading of antibiotic resistance by the action on adhesion-mediated virulence factors in Staphylococcus aureus

Papa, Rosanna; Artini, Marco; Cellini, Andrea; Tilotta, Marco; Galano, Eugenio; Pucci, Piero; Amoresano, Angela; Selan, Laura

doi:10.1016/j.micpath.2013.05.003

Cited by 42 publications

(44 citation statements)

References 58 publications

(45 reference statements)

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“…The Chlorhexidine MIC defined in this study was equal to or higher than 4 µg/mL. According to previous studies (16)(17)(18), the Chlorhexidine MIC for MRSA strains was typically equal to or higher than 4 µg/mL. Therefore, our finding was in accordance with those of the abovementioned studies.…”

Section: Discussionsupporting

confidence: 91%

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Detection of Antiseptic Resistance Genes among Staphylococcus aureus Colonising Nurses and Coagulase-Negative Staphylococci Isolated from Clinical Specimens at Teaching Hospitals in Southwest of Iran

Damavandi

Dehkordi

Dehghan

et al. 2016

Jundishapur J Microbiol

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Background: The wide application of antibiotics and antiseptics for patient therapy and medical equipment and surfaces disinfection has resulted in the emergence of resistant microorganisms. Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) are found as a part of the normal resident flora in human so that up to two-thirds of the healthy populations are permanently or transiently colonized by S. aureus and CoNS. Chlorhexidine is an antiseptic agent particularly effective against Gram-positive bacteria. It is widely used for hygienic hand wash to prevent transmission of Staphylococci nosocomial infections. The plasmid-borne qacA/B, qacC, and smr genes confer resistance to cationic antiseptic agents in S. aureus and CoNS. Objectives: The objective of the current study was to characterize the antibiotic resistance and susceptibility to quaternary ammonium compounds (QACs) in methicillin-sensitive S. aureus (MSSA), methicillin-resistant S. aureus (MRSA), methicillin-sensitive coagulase-negative staphylococci (MSCoNS), and methicillin-resistant coagulase-negative Staphylococci (MRCoNS). Methods: In this study, the antibiotic susceptibility and resistance to Chlorhexidine in 120 Staphylococcal strains were evaluated by disc diffusion and Minimum inhibitory concentration (MIC) of Chlorhexidine gluconate (CHG) methods, respectively. The MICs of CHG were determined in triplicate by broth micro-dilution, and the presence of mecA, qacA/B, qacC, and smr genes was examined by PCR assay. Results: Of total 60 S. aureus isolates, 51 (85%) were MRSA, and of 60 CoNS, 7 (11.66%) were MRCoNS. The results showed that the MIC

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Section: Discussionsupporting

confidence: 91%

“…Staphylococcus aureus is a flexible pathogen, which has been known as a common cause of various communityacquired and nosocomial infections (18). Hospital staff nasal carriers of MRSA can increase the risk of outbreaks of MRSA at Hospitals because they are potential reservoirs for the spread of MRSA strains among patients.…”

Section: Discussionmentioning

confidence: 99%

Detection of Antiseptic Resistance Genes among Staphylococcus aureus Colonising Nurses and Coagulase-Negative Staphylococci Isolated from Clinical Specimens at Teaching Hospitals in Southwest of Iran

Damavandi

Dehkordi

Dehghan

et al. 2016

Jundishapur J Microbiol

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“…19 Each flow channel connects to an input well (inlet) and an output well (outlet) on the plate. To grow biofilm in the BioFlux system, the channels were first primed.…”

Section: Dynamic Biofilm Assaymentioning

confidence: 99%

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Parrilli

Papa²,

Carillo

et al. 2015

Int J Immunopathol Pharmacol

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Staphylococcus epidermidis is recognized as cause of biofilm-associated infections and interest in the development of new approaches for S. epidermidis biofilm treatment has increased. In a previous paper we reported that the supernatant of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 presents an anti-biofilm activity against S. epidermidis and preliminary physico-chemical characterization of the supernatant suggested that this activity is due to a polysaccharide. In this work we further investigated the chemical nature of the anti-biofilm P. haloplanktis TAC125 molecule. The production of the molecule was evaluated in different conditions, and reported data demonstrated that it is produced in all P. haloplanktis TAC125 biofilm growth stages, also in minimal medium and at different temperatures. By using a surface coating assay, the surfactant nature of the anti-biofilm compound was excluded. Moreover, a purification procedure was set up and the analysis of an enriched fraction demonstrated that the anti-biofilm activity is not due to a polysaccharide molecule but that it is due to small hydrophobic molecules that likely work as signal. The enriched fraction was also used to evaluate the effect on S. epidermidis biofilm formation in dynamic condition by BioFlux system.

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“…Serratiopeptidase modulates adhesion expression in some bacterial species and enhances antibiotic efficacy toward biofilm-forming bacteria [23][24][25]. The antibiofilm formation property of serratiopeptidase helps in enhancing antibiotic efficacy in the treatment of staphylococcal infections [26].…”

Section: Mechanism Of Actionmentioning

confidence: 99%

The Emerging Role of Serratiopeptidase in Oral Surgery: Literature Update

Kumar

2018

Asian J Pharm Clin Res

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Serratiopeptidase, a proteolytic enzyme derived from Serratia E-15 species enterobacteria, is widely used in medical field for its anti-inflammatory, anti-edemic properties, and analgesic properties. It is being used commonly in various specialties such as orthopedics, otolaryngology, gynecology, surgery, pulmonology, ophthalmology, and dentistry. Research has shown that serratiopeptidase is the most effective anti-inflammatory agent compared to other enzyme preparations. This article reviews the efficacy, safety, and applications of serratiopeptidase in oral surgery. This article also discusses the mechanism of action of serratiopeptidase, its contraindications and complications. From the recently published literature, it is clear that the role of serratiopeptidase as a therapeutic agent in oral and maxillofacial surgery is expanding and they hold a promising future as a broad-spectrum anti-inflammatory drug with minimal side effects and complications. Further, research will broaden their applications in the field of medicine and dentistry.

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A new anti-infective strategy to reduce the spreading of antibiotic resistance by the action on adhesion-mediated virulence factors in Staphylococcus aureus

Cited by 42 publications

References 58 publications

Detection of Antiseptic Resistance Genes among Staphylococcus aureus Colonising Nurses and Coagulase-Negative Staphylococci Isolated from Clinical Specimens at Teaching Hospitals in Southwest of Iran

Detection of Antiseptic Resistance Genes among Staphylococcus aureus Colonising Nurses and Coagulase-Negative Staphylococci Isolated from Clinical Specimens at Teaching Hospitals in Southwest of Iran

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

The Emerging Role of Serratiopeptidase in Oral Surgery: Literature Update

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