A new and simple resonance Rayleigh scattering method for human serum albumin using graphite oxide as probe

Abstract

Graphite oxide (GO) was prepared by the Hummer procedure, and can be dispersed to stable colloid solution by ultrasonic wave. The GO exhibited an absorption peak at 313 nm, and a resonance Rayleigh scattering (RRS) peak at 490 nm. In pH 4.6 HAc-NaAc buffer solution, human serum albumin (HSA) combined with GO probe to form large HSA-GO particles that caused the RRS peak increasing at 490 nm. The increased RRS intensity was linear to HSA concentration in the range 0.50-200 µg/mL. Thus, a new and simple RRS method was proposed for the determination of HSA in samples, with a recovery of 98.1-104%.

A Sensitive Silver Nanorod/Reduced Graphene Oxide SERS Analytical Platform and Its Application to Quantitative Analysis of Iodide in Solution

“…Graphene oxide (GO) nanosheets have many excellent physical and chemical properties and have been intensively researched with a view to producing new devices in many fields [32][33][34]. GOs contain epoxide, carboxyl, and hydroxyl groups on their basal planes and edges, the abundance carboxyls coordinate with Ag + to form stable GO-Ag n + complex.…”

“…Preparation of the AgNR/rGO Sol GO can be prepared easily by Hummer procedure [32,33] and AgNR sol was prepared by simple chemical reduction [34]. Using CTAB surfactant as the template and small naosilver as seeds, the AgNR colloids were prepared commonly by vitamin C chemical reduction [34].…”

“…A 1.0 mol/L HCl solution, 1.0 % trisodium citrate, 0.010 mol/L Rh6G (Merck company, USA) stock solution, 1 mol/L NaCl solution, 2.4×10 −2 mol/L AgNO 3 solution, 1.00×10 −2 mol/L KI stock solution, 10 mmol/L vitamine C (VC), and 0.25 mg/mL NaBH 4 solutions were used. Graphene oxide (GO) nanosheets were prepared by Hummer procedure [32,33]. All reagents were of analytical grade and the water was doubly distilled.…”

Immunocontrolling Graphene Oxide Catalytic Nanogold Reaction and Its Application to SERS Quantitative Analysis

“…27 − 29 He et al 27 reported that GO prepared by the Hummers method could catalyze the hydrolysis of soybean isoflavones. In the nanoanalysis, 30 − 38 Wang et al established a resonance Rayleigh scattering (RRS) method to detect HSA, using GO as a probe. 30 The SERS effect of GO was studied by Hao et al 31 The SERS properties of GO/Au/Ag composites were better than those of pure gold and silver nanoparticles.…”

“…In the nanoanalysis, 30 − 38 Wang et al established a resonance Rayleigh scattering (RRS) method to detect HSA, using GO as a probe. 30 The SERS effect of GO was studied by Hao et al 31 The SERS properties of GO/Au/Ag composites were better than those of pure gold and silver nanoparticles. Banchelli’s research group found that GO–Ag composite nanoparticles were more effective than silver nanomaterials when used as a substrate in SERS analysis.…”