A New and Highly Sensitive Spectrophotometric Determination of Monocrotophos in Environmental, Agricultural and Biological Samples

Janghel, E. K.; K., J.; K., M.; Gupta, V. K.

doi:10.1002/jccs.200600043

Cited by 13 publications

(8 citation statements)

References 18 publications

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“…Calibration curves for both the pesticides were prepared using standard solutions of pesticides containing 2-10 mg/L of MCP and CPS. The complexing agents used for determination of MCP and CPS were diazotized with p-amino acetophenone and anthranilic acid, respectively [21,22]. The photodegradation efficiency of MCP and CPS was calculated as follows:…”

Section: Methodsmentioning

confidence: 99%

Photocatalytic degradation of monocrotophos and chlorpyrifos in aqueous solution using TiO2 under UV radiation

Amalraj

Pius

2015

Journal of Water Process Engineering

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Section: Methodsmentioning

confidence: 99%

Photocatalytic degradation of monocrotophos and chlorpyrifos in aqueous solution using TiO2 under UV radiation

Amalraj

Pius

2015

Journal of Water Process Engineering

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“…Molecularly identified fungal strain Aspergillus niger JQ660373 was used in the present study [16]. In the chemical method 0.1 N NaOH was used, as alkaline hydrolysis is a common method for the degradation of MCP [27]. 1 ml each of 0.1 N NaOH, fungal spore suspension and purified enzyme fraction was inoculated in 125ml of phosphorus free CZM medium for 10 days.…”

Section: Enzymatic Methodmentioning

confidence: 99%

Comparative Analysis of Chemical, Fungal and Enzymatic Degradation of MCP

Jain¹,

Garg²

2014

Scholarena Journal of Biotechnology

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In general three different methods are used for the cleanup of pesticides. These include chemical, microbial and enzymatic methods. Therefore, the present study is an endeavor to compare the efficiency of all the three methods for the study of degradation of monocrotophos (MCP) (150 µgml -1 ). Chemical method involved the use of 0.1N NaOH, whereas for the biological as well as enzymatic method, molecularly characterized fungal strain Aspergillus niger JQ660373 was used. %age of MCP degradation was assessed in the form of residual MCP concentration and hence degradation kinetics was premeditated. The results were confirmed by using analytical tools, High Performance Thin Layer Chromatography (HPTLC) and Fourier Transfom Infrared (FTIR). Results of the study demonstrated that degradation of MCP followed first order kinetics with the calculated half-life of 0.79 days in enzymatic method as compared to 3.16 and 4.21 days for fungal and chemical method. HPTLC chromatograms clearly indicate significant decrease in the standard MCP peak (rf 0.19-0.21) with increasing incubation duration. Molecular insight of MCP degradation as studied, suggests hydrolytic cleavage of MCP resulting in the formation of inorganic phosphates (-PO 4 ) and amines. The overall efficiency of degradation was found to be Enzymatic > Fungal > Chemical. The term "pesticide" embraces an enormous diversity of products that are used in a number of different activities and are intended for preventing, destroying, repelling or mitigating pests. Among newly developed pesticides, organophosphorus pesticides (OPPs) are most commonly used as they degrade faster in the environment. MCP (Dimethyl (E)-1-methyl-2 methyl carbamoyl vinyl phosphate) is an organophosphate with broad-spectrum contact and systemic activity against spur throated locust, sorghum midge, western flower thrips, aphids, the green vegetable bug, mites, the stem borer, potato tuber moth which are common pests of sorghum, sunflowers, tomatoes, cotton, potato, soybean and tobacco [1,2].Despite increasing crop productivity MCP is highly toxic to human beings. Mechanism of toxicity of MCP involves irreversible acetyl cholinesterase inhibition [3].Owing to its highly toxic nature, degradation of MCP from the environment is very essential. Degradation of MCP occurs by biotic as well as abiotic factors of the natural environment. These factors influence the degradation of MCP and act in tandem and complement one another in the microenvironment [4].Traditional cleanup methods of pesticides include the use of chemicals. These chemicals pose hazards on the soil fertility and soil microbial flaura and fauna. Therefore, these methods were discarded and biological methods emerged as a new tool for the degradation of pesticides.

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“…Determination of monocrotophos in cell free fermented mineral salts medium After periodic intervals of 24 hours the fermented broth was centrifuged at 8000 rpm for 10 min and the supernatant samples were collected and filtered through a 0.45 micron membrane filter paper. Amount of monocrotophos in the filtered samples was estimated by method of Janghel et al 2006. Monocrotphos degradation kinetics of Kocuria turfanesis For the study of degradation kinetics of Kocuria turfanesis strain BS-J pure culture of MCP1 was suspended in 1 ml 0.9% saline to make a cell suspension of 1×108 cells per ml and 100μl of this suspension was inoculated in 125 ml of M1 medium containing 150 mg L-1 concentration of monocrotophos and incubated at 28±2 °C for 15 days in an orbital shaking incubator at 90 rpm under aerated culture conditions.…”

Section: Methodsmentioning

confidence: 99%

Ecology of Little Green Bee Eater (Merops Orientalis) From Nagpur Area (India)

P…¹

2014

IJRBAT

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The ability of the biosurfactant producing Kocuria turfanesis to mineralize monocrotophos was investigated through an experimental set up using different treatments of soil with carrier based bioaugmentation package of egg shell coated with biosurfactant and biosurfactant producing microbial isolates BS-J cells. Dual capabilities of biosurfactant production and degradation of monocrotophos are the two main attributes of strain BS-J which was isolated from lube oil and distillery spent wash contaminated soil collected from a distillery unit. On the basis of the cellular morphology, physiological and chemotaxonomic characteristics and phylogenetic similarity of 16S rDNA gene sequences, the strain BS-J was identified as a Kocuria turfanesis. Present study has shown that degradation of monocrotophos in contaminated soil was facilitated after using carrier based bioaugmentation package of egg shell coated with biosurfactant producing microbial isolate BS-J cells and its biosurfactant resulted 86-89 % degradation of monocrotophos after 7-days of treatment. Without biosurfactant producing microbial cells the degradation of pesticide was comparatively lower indicating the need of live biosurfactant producing microbial cells and their surfactants for efficient degradation of pesticide. Results indicate that Kocuria turfanesis strain BS-J has great potential utility for the bioremediation of soil contaminated with monocrotophos.

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A New and Highly Sensitive Spectrophotometric Determination of Monocrotophos in Environmental, Agricultural and Biological Samples

Cited by 13 publications

References 18 publications

Photocatalytic degradation of monocrotophos and chlorpyrifos in aqueous solution using TiO2 under UV radiation

Photocatalytic degradation of monocrotophos and chlorpyrifos in aqueous solution using TiO2 under UV radiation

Comparative Analysis of Chemical, Fungal and Enzymatic Degradation of MCP

Ecology of Little Green Bee Eater (Merops Orientalis) From Nagpur Area (India)

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