A new and efficient method to generate human IgG monoclonal antibodies reactive to cancer cells using SCID-hu mice

Satoh, N.; Kudo, Tetsuichi; Saeki, Hisaaki; Saijo, S.; Hasumi, Toru; Yoshida, Hiroyuki; Kobayashi, Shunsuke; Fujimura, Shigefumi

doi:10.1016/0165-2478(95)00080-o

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…This shows that the human B cells or plasma cells in the marrow implanted along with the bone remain viable and continue to produce immunoglobulin for an extended period after implantation. These findings are in agreement with previous studies that reported the detection of human antibodies in the serum of mice implanted with human hematopoietic cells (25).…”

Section: Resultssupporting

confidence: 83%

A Mouse Model of Human Breast Cancer Metastasis to Human Bone

et al. 2005

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Currently, an in vivo model of human breast cancer metastasizing from the orthotopic site to bone does not exist, making it difficult to study the many steps of skeletal metastasis. Moreover, models used to identify the mechanisms by which breast cancer metastasizes to bone are limited to intracardiac injection, which seeds the cancer cells directly into the circulation, thus bypassing the early steps in the metastatic process. Such models do not reflect the full process of metastasis occurring in patients. We have developed an animal model of breast cancer metastasis in which the breast cancer cells and the bone target of osteotropic metastasis are both of human origin. The engrafted human bone is functional, based on finding human IgG in the mouse bloodstream, human B cells in the mouse spleen, and normal bone histology. Furthermore, orthotopic injection of a specific human breast cancer cell line, SUM1315 (derived from a metastatic nodule in a patient), later resulted in both bone and lung metastases. In the case of bone, metastasis was to the human implant and not the mouse skeleton, indicating a species-specific osteotropism. This model replicates the events observed in patients with breast cancer skeletal metastases and serves as a useful and relevant model for studying the disease. (Cancer Res 2005; 65(14): 6130-38)

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Section: Resultssupporting

confidence: 83%

A Mouse Model of Human Breast Cancer Metastasis to Human Bone

et al. 2005

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“…In 95% of the engrafted bone implants, marrow spaces were preserved. As an index of marrow functionality, we measured human IgG levels in blood of mice that received bone implants 6 and 10 weeks earlier: ELISA demonstrated that human IgG was present in abundance (∼100 μg/mL), indicating that the human B cells or plasma cells in implanted marrow remain viable and produce immunoglobulin for an extended period, as previously observed 69 …”

Section: An “All Human” Animal Model Of Breast Cancer Metastasismentioning

confidence: 63%

Studies of Osteotropism on Both Sides of the Breast Cancer–Bone Interaction

Moreau

Anderson

Mauney

et al. 2007

Annals of the New York Academy of Sciences

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While important advances have been made in the treatment of breast cancer (BrCa), little progress has been made in developing therapies for metastasis to bone, a complication that signals entry of the disease into an incurable phase. The process of identifying genes and gene signatures of BrCa associated with metastasis has begun. In contrast, knowledge of the contributions of bone to tumor-stroma interaction is still rudimentary. We are performing research designed to elucidate the mechanisms by which human BrCa metastasizes to bone (osteotropism). With evidence mounting that there is mutual recognition of BrCa and bone, we are investigating osteotropism from both sides of the tumor-stroma interface. We created a novel "all human" model in which human bone is transplanted into immunodeficient (NOD/SCID) mice. Human BrCa cells are injected into the mammary fat pad. Metastases later appear as metastases in the human bone, but not mouse skeleton. The model recapitulates the metastatic sequence occurring in patients. Using DNA microarrays, we plan to identify putative osteotropic genes expressed by metastatic BrCa cells. We will test the hypothesis that distinct "tool kits" are used by BrCa metastasizing to human bone. In addition, using human tissue-engineered bone, we are identifying components within bone stroma essential for metastasis, and osteotropism genes expressed by bone in response to the presence of BrCa. We recently demonstrated that tissue-engineered bone based on a silk sponge platform is a target for human BrCa metastasis, even in preference to the mouse skeleton.

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“…AsA are also capable of altering capacitation and acrosomal reactions and may block sperm-oocyte interactions. [6][7][8] Two strains of mice have been employed: severe combined immunodeficiency mice-SCID: (C.B.17 scid ⁄ scid) and NOD ⁄ SCID (NOD ⁄ LTSz-scid ⁄ scid; 3 Little is known about the specific mechanisms that elicit auto-and isoimmune reac-tions to sperm in humans.…”

Section: Introductionmentioning

confidence: 99%

“…The SCID mouse model is an excellent alternative to in vitro cultures and one of the best animal models ever found for studying the immunological reactions in disease. [6][7][8] Two strains of mice have been employed: severe combined immunodeficiency mice-SCID: (C.B.17 scid ⁄ scid) and NOD ⁄ SCID (NOD ⁄ LTSz-scid ⁄ scid;…”

Section: Introductionmentioning

confidence: 99%

ORIGINAL ARTICLE: In situ Reconstruction of Humoral Immune Response Against Sperm: Comparison of SCID and NOD/SCID Mouse Models

Grygielska

Kamieniczna

Wiland

et al. 2009

American J Rep Immunol

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NOD/SCID mice inoculated with hu-PBLs exhibited higher AsA titres with a tendency for greater sperm agglutination than human AsA raised in SCID mouse model. A comparison between 'native' and deglycosylated sperm extracts revealed higher agglutination titres by sera induced with the latter ones. Inhibitory effect of human polyclonal AsA in sperm penetration assay, however, produced opposite results to that for agglutination. Western immunoblotting was used to evaluate reactive sperm antigens prior to and after in situ sensitization showing multiple bands different from positive reactions brought by original sera of in vivo primed AsA-positive males. It seems that in situ generated AsA recognized sperm entities different from those revealed by blood donor's sera samples.

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A new and efficient method to generate human IgG monoclonal antibodies reactive to cancer cells using SCID-hu mice

Cited by 5 publications

References 17 publications

A Mouse Model of Human Breast Cancer Metastasis to Human Bone

A Mouse Model of Human Breast Cancer Metastasis to Human Bone

Studies of Osteotropism on Both Sides of the Breast Cancer–Bone Interaction

ORIGINAL ARTICLE: In situ Reconstruction of Humoral Immune Response Against Sperm: Comparison of SCID and NOD/SCID Mouse Models

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