A new amperometric enzyme electrode for alcohol determination

Gülce, Handan; Gülce, Ahmet; Kavanoz, Muammer; Çoşkun, H.Ş.; Yıldız, Attila

doi:10.1016/s0956-5663(02)00008-8

Cited by 62 publications

(31 citation statements)

References 18 publications

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“…65 Quantitative determination of alcohol has been essential in food, fermentation, and wine industries; alcohol oxidase therefore has had broad substrate specificity and has oxidized a variety of short-chain primary alcohols, consuming oxygen and generating hydrogen peroxide. 58 Microbial/electrochemical oxygen and peroxide electrodes for alcohol determination were proposed to enhance the alcohol activity and to impair the catalase activity by using appropriate mutants of methylotrophic yeasts Hansenula polymorpha, the sensitive bioelements. 67 The selected mutations used for the oxygen electrodes allowed an increase in the respiration response of mutant cells towards alcohols, whereas in the case of the cells used with a peroxide biosensor, the mutant cells elevated the excretion of hydrogen peroxide during metabolic transformation of alcohol.…”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…55,56,58,69 The redox polymer, polyvinylferrocenium perchlorate, behaved like a modified surface through which an electron transfer between a substrate and a reactant took place. 2,55 The polyvinylferrocenium-coated electrode was simple, fast, sensitive, and stable and had a constant and maximum response time at the temperature of physiological fluids.…”

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

“…56 A new amperometric enzyme electrode in polyvinylferrocenium perchlorate was proposed for the development of the alcohol biosensor. 58 The sensitivity of the enzyme electrode was based on four types of alcohol: methanol, ethanol, 1-butanol, and benzyl alcohol. It was observed that the sensitivity of the enzyme electrode reduced to 6.2 mM for 1-butanol and 3.0 mM for ethanol.…”

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

“…The effects of several possible interferences from cholesterol, sucrose, Dglucose, trozin, D-fructose, D-galactose, lactate, L-tyrosine, and ascorbic acid were tested in 0.10 M buffer solution of pH 8.0 at 30˚C; the interferences from trozin and ascorbic acid, however, were the most noticeable ones at the concentration of 5 mM. 58 A new enzyme electrode for the determination of galactose was developed, and the measurements were performed using this with an amperometric biosensor based on the immobilization of galactose oxidase in polyvinylferrocenium matrices. 59 The polyvinylferrocenium-coated electrodes offered the same advantages as those of the previous studies.…”

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

“…2,55,60 The electrode materials of conducting salts are often used for reactions involving NAD(P) + -dependent dehydrogenases, as they allow the electrochemical oxidation of the reduced forms of these coenzymes. 58,59 Much work has also been done in recent years on the applications of conducting polymer layers in amperometric biosensors and amperometric immunosenors. 9,31,[61][62][63] In the next section, the applications of the amperometric principles are discussed to illustrate some recent results of the intense research for more selective, sensitive, and reliable biological sensors.…”

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confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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The objective of this study is to analyze the technical importance, performance, techniques, advantages, and disadvantages of the biosensors in general and of the electrochemical biosensors in particular. A product of reaction diffuses to the transducer in the first generation biosensors (based on Clark biosensors). The mediated biosensors or second generation biosensors use specific mediators between the reaction and the transducer to improve sensitivity. The second generation biosensors involve two steps: first, there is a redox reaction between enzyme and substrate that is reoxidized by the mediator, and eventually the mediator is oxidized by the electrode. No normal product or mediator diffusion is directly involved in the third generation biosensors, direct biosensors. Based on the type of transducer, current biosensors are divided into optical, mass, thermal, and electrochemical sensors. They are used in medical diagnostics, food quality controls, environmental monitoring, and other applications. These biosensors are also grouped under two broad categories of sensors: direct and indirect detection systems. Moreover, these systems could be further grouped into continuous or batch operation. Therefore, amperometric biosensors and their current applications are focused on more in detail since they are the most commonly used biosensors in monitoring and diagnosing tests in clinical analysis. Problems related to the commercialization of medical, environmental, and industrial biosensors as well as their performance characteristics, their competitiveness in comparison to the conventional analytical tools, and their costs determine the future development of these biosensors.

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Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

mentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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Investigation of the behavior of hydrogen‐bonded phenolic compounds and their determination by using poly(vinylferrocenium)–polyaniline composite film

Kavanoz

Pekmez

Can

2016

J of Applied Polymer Sci

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ABSTRACT:The hydrogen bonding between phenolic compounds (phenol (Ph), catechol (Ct), resorcinol (Rs), and hydroquinone (Hq)) is investigated at pH 4. The oxidation behaviors of total phenolic compounds (TotPh) are different from their individual behaviors due to the existence of intermolecular hydrogen-bonded oligomeric clusters. Theoretical calculations and voltammetric and spectroscopic evidences support the intermolecular hydrogen bonding. The interaction of the phenolic compounds with polyaniline (PANI) and poly(vinylferrocenium) (PVF 1 ) films are also investigated electrochemically and spectroscopically. The phenolic molecules are immobilized in both polymers due to the construction of hydrogen bonds by PANI and the complexation with PVF

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Alcohol biosensing by polyamidoamine (PAMAM)/cysteamine/alcohol oxidase‐modified gold electrode

Akin

Yüksel

Geyik

et al. 2010

Biotechnology Progress

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A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4 degrees C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation.

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A new amperometric enzyme electrode for alcohol determination

Cited by 62 publications

References 18 publications

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Investigation of the behavior of hydrogen‐bonded phenolic compounds and their determination by using poly(vinylferrocenium)–polyaniline composite film

Alcohol biosensing by polyamidoamine (PAMAM)/cysteamine/alcohol oxidase‐modified gold electrode

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