A new alternative method for testing skin irritation using a human skin model: A pilot study

Miles, Alexandra; Berthet, Aurélie; Hopf, Nancy B.; Gilliet, Michel; Raffoul, Wassim; Vernez, David; Spring, Philipp

doi:10.1016/j.tiv.2013.10.022

Cited by 18 publications

(13 citation statements)

References 25 publications

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“…According to Greaves (2012), the epidermal layer will not erode but changes like hyperkeratosis and acanthosis can occur when the irritation intensity is mild or moderate. Miles et al (2014) defined non irritant substances as those that induce slight or no damage to the skin, while irritants induce severe damage ranging from spongiosis to epidermolysis. In this study, the P. aduncum EO cream formulation only induced slight irritation to rabbit skin; thus, the epidermal layer was well defined, without the presence of hyperkeratosis or acanthosis.…”

Section: Discussionmentioning

confidence: 99%

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Mamood¹,

Othman²,

Nasir³

et al. 2017

American Journal of Pharmacology and Toxicology

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Abstract:It has been shown in previous research that Piper aduncum Linnaeus essential oil has the potential to be developed as an alternative mosquito repellent. When the essential oil was formulated into cream, it was able to provide >2h of protection against Aedes aegypti in the laboratory; thus, it can be commercialized as an alternative to synthetic repellent especially N, N-diethyl-3-methylbenzamide (DEET). In this study, the irritation and sensitization potential of a cream formulation containing P. aduncum essential oil was investigated to verify its safety for application purposes. The P. aduncum essential oil was formulated into a cream containing 10% of the essential oil for irritation and skin sensitization assays on New Zealand white rabbits and guinea pigs (Hartley strain), respectively, following the ISO10993-10:2010 (E) guidelines. The macroscopic and histological observations from both assays revealed that the cream formulation containing P. aduncum essential oil caused slight irritation on rabbit skin, with a Primary Irritation Index (PII) of 1.54; however, no positive response was detected in the skin sensitization assay. In conclusion, the cream formulation containing 10% P. aduncum essential oil was slightly irritating to rabbit skin but did not cause sensitization in the animals tested.

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Section: Discussionmentioning

confidence: 99%

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Mamood¹,

Othman²,

Nasir³

et al. 2017

American Journal of Pharmacology and Toxicology

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“…The study was approved by the Ethics Commission of the State of Vaud, Switzerland (protocol 264/12). The potential toxicity of Ag and Cu nanoparticles for the skin was evaluated ex vivo in a human skin irritation model, using a 6 flow-through diffusion cell system (PermeGear; SES Analytical Systems, Bechenheim, Germany) (28).…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Effectiveness of an Innovative Silver-Copper Nanoparticle Coating of Catheters To Prevent Methicillin-Resistant Staphylococcus aureus Infection

Ballo

Rtimi

Pulgarín

et al. 2016

Antimicrob Agents Chemother

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In this study, silver/copper (Ag/Cu)-coated catheters were investigated for their efficacy in preventing methicillin-resistant Staphylococcus aureus (MRSA) infection in vitro and in vivo. Ag and Cu were sputtered (67/33% atomic ratio) on polyurethane catheters by direct-current magnetron sputtering. In vitro, Ag/Cu-coated and uncoated catheters were immersed in phosphatebuffered saline (PBS) or rat plasma and exposed to MRSA ATCC 43300 at 10 4 to 10 8 CFU/ml. In vivo, Ag/Cu-coated and uncoated catheters were placed in the jugular vein of rats. Directly after, MRSA (10 7 CFU/ml) was inoculated in the tail vein. Catheters were removed 48 h later and cultured. In vitro, Ag/Cu-coated catheters preincubated in PBS and exposed to 10 4 to 10 7 CFU/ml prevented the adherence of MRSA (0 to 12% colonization) compared to uncoated catheters (50 to 100% colonization; P < 0.005) and Ag/Cu-coated catheters retained their activity (0 to 20% colonization) when preincubated in rat plasma, whereas colonization of uncoated catheters increased (83 to 100%; P < 0.005). Ag/Cu-coating protection diminished with 10 8 CFU/ml in both PBS and plasma (50 to 100% colonization). In vivo, Ag/Cu-coated catheters reduced the incidence of catheter infection compared to uncoated catheters (57% versus 79%, respectively; P ‫؍‬ 0.16) and bacteremia (31% versus 68%, respectively; P < 0.05). Scanning electron microscopy of explanted catheters suggests that the suboptimal activity of Ag/Cu catheters in vivo was due to the formation of a dense fibrin sheath over their surface. Ag/Cu-coated catheters thus may be able to prevent MRSA infections. Their activity might be improved by limiting plasma protein adsorption on their surfaces. T he use of intravenous catheters (IVCs) in patients is often associated with the development of bloodstream infection (BSI), metastatic abscesses, and infective endocarditis (1, 2), among other infections. These infections contribute to substantial health care-associated morbidity, prolonged hospital stay, and increased costs (3, 4).IVCs become colonized predominantly by microorganisms present on the skin, most often at the time of intravascular insertion (5, 6). IVC-related infections are most commonly caused by staphylococci, including Staphylococcus aureus, and are particularly problematic in the case of methicillin-resistant S. aureus (MRSA) (7-9).Measures have been developed so far to prevent IVC-related infections (4). Priority is given to hygiene, including maximal barrier precautions during IVC insertion such as skin antisepsis by alcohol disinfection (10), hand hygiene, strict aseptic insertion procedures, and sometimes the use of antiseptic-or antibioticimpregnated dressings or catheters (11,12).The use of aseptic procedures helps in preventing insertionrelated IVC infections (4, 13). However, these measures are limited by practical compliance problems (14). A number of clinical trials have demonstrated that the use of antimicrobial-coated catheters reduce the incidence of S. aureus IVC infections and subsequent BSI...

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“…Also, the hydrolipid film cannot be totally reproduced regarding its bio-physiological properties of components (urea, glycolic acid) neither to the occlusive effect of the film's lipid component (ceramides) (Miles et al, 2014). Some cells normally present in human skin are not present in this model such as dendritic Langerhans' cells.…”

Section: Extractmentioning

confidence: 98%

“…According to Miles et al (2014), reconstructed human epider-mis like EpiSkin TM has some limitations. This model is composed Table 3 SkinEthic TM HCE model was exposed for 10 min to controls and extracts.…”

Section: In Vivo Testmentioning

confidence: 99%

“…of a highly differentiated multi-layer human epidermis cells, but are totally lacking in any network of the pilo-sebaceous units, which regulate dermal homeostasis (Miles et al, 2014). Also, the hydrolipid film cannot be totally reproduced regarding its bio-physiological properties of components (urea, glycolic acid) neither to the occlusive effect of the film's lipid component (ceramides) (Miles et al, 2014).…”

Section: Extractmentioning

confidence: 99%

See 1 more Smart Citation

Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach

Rodrigues

Pereira

Pimentel

et al. 2015

Industrial Crops and Products

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Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant con-stituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredi-ent, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin TM , and human corneal epithelial model, SkinEthics TM HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applica-tions was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application.

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A new alternative method for testing skin irritation using a human skin model: A pilot study

Cited by 18 publications

References 25 publications

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

In Vitro and In Vivo Effectiveness of an Innovative Silver-Copper Nanoparticle Coating of Catheters To Prevent Methicillin-Resistant Staphylococcus aureus Infection

Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach

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