A new allele in the BMP15 gene (FecX) that affects prolificacy co-segregates with FecX and FecX in Rasa aragonesa sheep

Calvo, J.H.; Chantepie, Louise; Serrano, M.; Sarto, María Pilar; Iguácel, Laura Pilar; Jiménez, María Ángeles Sánchez; Alabart, J. L.; Folch, J.; Fabre, Stéphane; Crespo, Belén Lahoz

doi:10.1016/j.theriogenology.2020.01.010

Cited by 14 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As two important members of the NLR family, NLRP5 and NLRP9 play important roles in mammal reproduction [1,11,12]. However, little is known about their effects on litter size in ewes.…”

Section: Nlrp5 and Nlrp9 Polymorphismsmentioning

confidence: 99%

“…It is possible that a missense mutation from G to A at the g.60495363G > A locus may decrease its positive effects on oocyte development and follicular maturation. Little is known about the effects of NLRP9 on oocyte development or follicular maturation; however, researches demonstrated that NLRP9 was highly expressed in adult bovine oocytes rather than prepubertal animals [12] and was particularly highly expressed in sheep ovaries [7]. Additionally, two oocyte-specific NLRP genes in mammals, NLRP5 and NLRP9, were only expressed in mouse oocytes [28], which indicated that they have critical roles in ovary functions, such as oocyte development and follicular maturation.…”

Section: Reproductive Functions Of Gdf9 and Its Interactions With Nlrmentioning

confidence: 99%

“…Normally, ewes with one copy of the FecB mutation increase the rate of ovulation by 1.5 and litter size by 1, and ewes with two copies can significantly increase the rate of ovulation and litter size by 3 and 1.5 (summarized by Liu et al [9]). In addition, several other mutations in genes, including the growth differentiation factor 9 gene (GDF9) [10,11], bone morphogenetic protein 15 (BMP15) [12], BMP2, and BMP7 [13], were also reported to participate in sheep reproduction and lead to differences in litter size; all of these point mutations could be important genetic markers for sheep breeding.Therefore, in this study, we aim to explore the association between NLRP5 and NLRP9 single nucleotide polymorphisms (SNPs) with litter size. This information has not previously been elucidated but is expected to provide valuable genetic markers for sheep breeding.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep

Zhang

Tang

et al. 2020

Animals

View full text Add to dashboard Cite

Simple Summary:The NLR family pyrin domain-containing 5 (NLRP5) and NLRP9 genes are two important reproductive genes; however, their effects on litter size in sheep are unknown. In this study, we conducted population genetic and association analyses on five NLRP5 and NLRP9 loci of sheep. Our results suggested that a mutation in g.60495363G > A may decrease interactions of NLRP5 with proteins, such as the growth differentiation factor 9 (GDF9), whereas a mutation in g. g.59030623T > C may enhance the NLRP9-combining capacity with these proteins. Consequently, these mutations may lead to differences in ovulation rate and even litter size. Overall, this study provided useful genetic markers that can be used to improve sheep breeding.Abstract: Previous studies showed that the NLR family pyrin domain-containing 5 (NLRP5) and NLRP9 genes are two important reproductive genes; however, their effects on sheep litter size are unknown. Therefore, in this study, we first genotyped seven sheep breeds via the MassARRAY ® SNP system at the loci g.60495375A > G, g.60495363G > A, and g.60499690C > A in NLRP5, and g.59030623T > C and g.59043397A > C in NLRP9. Our results revealed that each locus in most sheep breeds contained three genotypes. Then, we conducted population genetic analysis of single nucleotide polymorphisms in NLRP5 and NLRP9, and we found that the polymorphism information content value in all sheep breeds ranged from 0 to 0.36, and most sheep breeds were under Hardy-Weinberg equilibrium (p > 0.05). Furthermore, association analysis in Small Tail Han sheep indicated that two loci, g.60495363G > A in NLRP5 and g.59030623T > C in NLRP9, were highly associated with litter size. The mutation in g.60495363G > A may decrease interactions of NLRP5 with proteins, such as GDF9, whereas the mutation in g.59030623T > C may enhance the combining capacity of NLRP9 with these proteins; consequently, these mutations may influence the ovulation rate and even litter size. The findings of our study provide valuable genetic markers that can be used to improve the breeding of sheep and even other mammals. zygotic development [1]. Additionally, a lack of NLRP5 in mouse oocytes resulted in premature activation of the mitochondrial pool, which results in mitochondrial damage that cannot be recovered by BCL2 associated X (Bax) inactivation [2], and NLRP5 knockout in mice led to female infertility [3]. Furthermore, preovulatory aging in mouse oocyte maturation decreased NLRP5 abundance, which indicated that NLRP5 has critical roles in oocyte development [4]. Notably, NLRP5, as a member of the sub-cortical maternal complex, was also found only expressed in ovine ovary and especially in the ovine oocytes in the germinal vesicle and metaphase II stage [5], which suggested their important roles in the oocyte developmental potential of sheep. However, its effects on sheep litter size are poorly understood.NLRP9 is also an important member of the NLR family. NLRP9 is highly detectable during in vitro maturation of bovine oocytes from small folli...

show abstract

“…As two important members of the NLR family, NLRP5 and NLRP9 play important roles in mammal reproduction [1,11,12]. However, little is known about their effects on litter size in ewes.…”

Section: Nlrp5 and Nlrp9 Polymorphismsmentioning

confidence: 99%

Section: Reproductive Functions Of Gdf9 and Its Interactions With Nlrmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep

Zhang

Tang

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…For example, point mutations in BMP15 (Calvo et al, 2020), GDF9 (Våge et al, 2013), MTNR1A (He et al, 2019), BMP7 and BMP2 (Zhang, Liu, et al, 2019) et al, 2014). These findings indicate that synonymous mutations have the potential to be valuable markers for improving sheep fecundity.…”

Section: Analysis Of the Association Between Tgif1 And Sf1 Polymorpmentioning

confidence: 99%

“…Mutations in a number of other genes have also been demonstrated to be useful genetic markers for sheep breeding. These genes include bone morphogenetic protein 15 (BMP15; Calvo et al, 2020), growth differentiation factor 9 (GDF9; Våge, Husdal, Kent, Klemetsdal, & Boman, 2013), melatonin receptor 1A (MTNR1A; , BMP7 and BMP2 (Zhang, Liu, et al, 2019). Such findings indicate that the identification of genetic markers can facilitate the progress of molecular marker-assisted breeding in sheep.…”

Section: Introductionmentioning

confidence: 99%

TGIF1 and SF1 polymorphisms are associated with litter size in Small Tail Han sheep

Zhang

Liu

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

TGF‐β induced factor homeobox 1 (TGIF1) and splicing factor 1 (SF1) are important for mammalian reproduction; however, the effects of these genes on litter size in sheep remain unexplored. In this study, we genotyped 768 ewes from seven sheep breeds at two loci: g.37871539C>T, a synonymous mutation of TGIF1; and g.42314637T>C, a 3′UTR variant of SF1. Our analysis of polymorphism revealed only two genotypes at locus g.37871539C>T in TGIF1, with most sheep populations being moderately polymorphic (0.25 < PIC < 0.5) at this site. In contrast, most breeds exhibited low polymorphism (PIC ≤0.25) at the SF1 locus g.42314637T>C. The association analysis revealed that a synonymous mutation at g.37871539C>T in TGIF1 was highly associated with litter size in Small Tail Han sheep, in which it causes a significant decrease in litter size. Conversely, while the SF1 3′UTR variant g.42314637T>C was also highly associated with litter size in sheep, it causes a significant increase in the number of litter size. Combined, these data provide valuable information regarding candidate genetic markers for sheep breeding programs.

show abstract

In silico analysis and molecular docking studies of natural compounds of Withania somnifera against bovine NLRP9

et al. 2023

View full text Add to dashboard Cite

Context NLRP9 is a member of nucleotide-binding domain leucine-rich repeat-containing receptors and is found to be associated with many inflammatory diseases. In the current scenario, the identification of promising anti-inflammatory compounds from natural sources by repurposing approach is still relevant for the early prevention and effective management of the disease. Methods In the present study, we docked bioactives of Ashwagandha (Withanoside IV, Withanoside V, Withanolide A, Withanolide B, and Sitoindoside IX) and two control drugs against bovine NLRP9 protein. ADME/T analysis was used to determine the physiochemical properties of compounds and standard drugs. Molecular modeling was used to evaluate the correctness and quality of protein structures. In silico docking analysis revealed Withanolide B had the highest binding affinity score of −10.5 kcal/mol, whereas, among control drugs, doxycycline hydrochloride was most effective (−10.3 kcal/mol). The results of this study revealed that bioactives of Withania somnifera could be promising inhibitors against bovine NLRP9. In the present study, molecular simulation was used to measure protein conformational changes over time. The Rg value was found to be 34.77A°. RMSD and B-factor were also estimated to provide insights into the flexibility and mobile regions of protein structure. A functional protein network interaction was constructed from information collected from non-curative sources as protein-protein interactions (PPI) that play an important role in determining the function of the target protein and the ability of the drug molecule. Thus, in the present situation, it is important to identify bioactives with the potential to combat inflammatory diseases and provide strength and immunity to the host. However, there is still a need to study in vitro and in vivo to further support these findings.

show abstract

A new allele in the BMP15 gene (FecX) that affects prolificacy co-segregates with FecX and FecX in Rasa aragonesa sheep

Cited by 14 publications

References 25 publications

Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep

Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep

TGIF1 and SF1 polymorphisms are associated with litter size in Small Tail Han sheep

In silico analysis and molecular docking studies of natural compounds of Withania somnifera against bovine NLRP9

Contact Info

Product

Resources

About