A new algorithm for high-resolution reconstruction of single particles by electron microscopy

An Asymmetric Opening of HIV-1 Envelope Mediates Antibody-Dependent Cellular Cytotoxicity

“…The following references appear in the Supplemental Information: Sorzano et al (2018); Kimanius et al (2016).…”

DeepRes: a new deep-learning- and aspect-based local resolution method for electron-microscopy maps

“…Note that the color scales of the resolution maps of the different specimens have been modified for better visualization. (Sorzano et al, 2018)]. Resolution changes determined with DeepRes before and after map sharpening are exemplified in Fig.…”

“…On the other hand, it is very common to introduce some form of map enhancement, from global B-factor correction to more complex post-processing operations such as modelbased or non-model-based sharpening [as in LocScale (Jakobi et al, 2017) and LocalDeblur (Ramírez-Aportela et al, 2019), respectively] or the introduction of nonlinear noise-suppression operations, as in Xmipp Highres (Sorzano et al, 2018). In all of these cases, our current resolution indicators lose effectivity: in the former case (B-factor correction) because they are insensitive to this operation (see the supporting information and Supplementary Fig.…”

“…Note that currently two maps, one sharpened and the other unsharpened, may have the same local resolution estimation but are certainly not judged as being the same by a human observer. An additional, more technical, motivation for our work on DeepRes was to have a way to calculate local resolution in those situations in which we did not have a proper noise estimation (as could be the case, for instance, when the reconstruction algorithm incorporates some noisesuppression prior, as in Xmipp Highres; Sorzano et al, 2018).…”

Advances in image processing for single-particle analysis by electron cryomicroscopy and challenges ahead

“…However, the issue is far from settled, particularly in those cases in which conformational changes correspond to a continuous distribution of states. This issue has been explored in some works [165,89] [166]. However, currently, there is no algorithm specifically designed with flexibility/heterogeneity in mind.…”