A New Algorithm for Deduction of Hepatitis B Surface Antigen Subtype Determinants from the Amino Acid Sequence

Purdy, Michael A.; Talekar, G; Swenson, Paul D.; Araujo, Aufra; Fields, Howard A.

doi:10.1159/000096312

Cited by 73 publications

(75 citation statements)

References 37 publications

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“…41 Interestingly, subtype adw3, which belonged to genotype C but had been officially recognized in genotype B and D, was first identified in Indonesia. 19,39 The major target of the neutralizing antibody produced by natural infection or vaccination is the a determinant within HBsAg. 42 The a determinant region represents the immunodominant HBsAg, which was initially considered as a conformational cluster of epitopes located between residues 120 and 150 of HBsAg and has currently been expanded to residues 110-160 after the discovery of two more epitopes.…”

Section: Discussionmentioning

confidence: 99%

“…HBV subtypes were then deduced on the basis of the predicted amino acid sequence substitution at positions 122, 127, 134, 140, 159, 160, and 177 of the S gene, as previously described. 9,10,19,25 Other amino acid substitutions in the S gene related to the vaccine-escape mutant were also analyzed.…”

Section: Methodsmentioning

confidence: 99%

“…[13][14][15][16] Ten HBV serological subtypes, which are based on the amino acid sequences of HBsAg, have been identified. [17][18][19] Indonesia has been geographically divided into four different HBV subtype zones: the adw, adr, ayw, and mix zones. 20 It is important to record the S gene variant type in an endemic population because this information may be critical for determining policies for vaccine and diagnostic reagent design.…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis B Virus Infection in Indonesia 15 Years After Adoption of a Universal Infant Vaccination Program: Possible Impacts of Low Birth Dose Coverage and a Vaccine-Escape Mutant

Purwono

Juniastuti

Amin

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. A universal hepatitis B vaccination program for infants was adopted in Indonesia in 1997. Before its implementation, the prevalence of hepatitis B surface antigen (HBsAg)-positive individuals in the general population was approximately 5-10%. The study aimed to investigate the hepatitis B virus (HBV) serological status and molecular profile among children, 15 years after adoption of a universal infant vaccination program in Indonesia. According to the Local Health Office data in five areas, the percentages of children receiving three doses of hepatitis B vaccine are high (73.9-94.1%), whereas the birth dose coverage is less than 50%. Among 967 children in those areas, the seropositive rate of HBsAg in preschool-and school-aged children ranged from 2.1% to 4.2% and 0% to 5.9%, respectively. Of the 61 HBV DNA-positive samples, the predominant genotype/subtype was B/adw2. Subtype adw3 was identified in genotype C for the first time in this population. Six samples (11.5%) had an amino acid substitution within the a determinant of the S gene region, and one sample had T140I that was suggested as a vaccine-escape mutant type. The low birth dose coverage and the presence of a vaccine-escape mutant might contribute to the endemicity of HBV infection among children in Indonesia.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hepatitis B Virus Infection in Indonesia 15 Years After Adoption of a Universal Infant Vaccination Program: Possible Impacts of Low Birth Dose Coverage and a Vaccine-Escape Mutant

Purwono

Juniastuti

Amin

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…Phylogenetic trees were constructed by the neighbor-joining method. HBV serotypes were deduced on the basis of predicted amino acid sequences of HBsAg [3,38,39] .…”

Section: Analysis Of Hbv Genotype/subgenotype Serotype and Hbx Mutatmentioning

confidence: 99%

Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Utama¹,

Purwantomo

Siburian

et al. 2009

WJG

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AIM:To identify the distribution of hepatitis B virus (HBV) subgenotype and basal core promoter (BCP) mutations among patients with HBV-associated liver disease in Indonesia. METHODS:Patients with chronic hepatitis (CH, n = 61), liver cirrhosis (LC, n = 62), and hepatocellular carcinoma (HCC, n = 48) were included in this study.HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing. RESULTS:HBV genotype B (subgenotypes B2, B3, B4, B5 and B7) the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C (subgenotypes C1, C2 and C3) was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 (84.9%) and C1 (82.2%) were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 (84.9%) and adrq+ (89.4%) were the most prevalent in HBV genotype B and C, respectively. Double mutation (A1762T/G1764A) in the BCP was significantly higher in LC (59.7%) and HCC (54.2%) than in CH (19.7%), suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC (46.8%), but lower in HCC (22.9%) and CH (18.0%), suggesting that this mutation may be an indicator of cirrhosis. CONCLUSION:HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.

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“…[7][8] Hepatitis B virus genotypes have specific distribution pattern in different geographical areas and present in different ethnic groups of the globe. Genotype A is mostly prevalent in South Africa, North America and West-North Europe, Genotype B in South-East Asia and genotype C in Korea, China, Vietnam and Japan.…”

Section: Introductionmentioning

confidence: 99%

Pre-Operative Surgical Patients; Serological Screening of Hepatitis B, Its Knowledge and Attitudes in Quetta, Pakistan

Umer¹,

Abbas

Shafee

et al. 2017

TPMJ

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ABSTRACT… Objectives: This study was designed to evaluate sero-prevalence of HBs Ag (Hepatitis-B surface antigen), its knowledge and attitudes in preoperative general surgery patients in Quetta, Pakistan. Study Design: A cross-sectional study. Place and Duration of Study: This study was carried out (April -September, 2016) and was screened in Balochistan Lab Brewery Road, Quetta. Methodology: 1000 preoperative patients from four ethnic groups predominantly present in surgical department of multiple hospitals of Quetta. Serum was subjected to chromatography immunoassay (ICT) and samples were reconfirmed through Enzyme linked immunosorbent assay. Demographical data, knowledge and attitudes were also collected using valid predesigned questionnaire. Results : Overall, prevalence 7.0% were observed with highest in Baloch population, 10.3 %, followed by Hazara community, 8.0 %, While Pashtoon were with 5.7 % and Panjabi population with 2.3 %. Statistically non-significant difference was observed in males and female population (P < 0.05). 03 of the patients were false negative with ICT when reconfirmed by ELISA. Overall, population show poor knowledge and attitudes in preoperative surgery patients toward Hepatitis B infection. Conclusion: The HBs Ag determination using rapid Immunochromatography device test should only be used for mass scale screening and results may be verified with ELISA to eliminate the chances of false negative cases. Key words:Hepatitis B, prevalence, ICT, ELISA, Quetta, preoperative patients.

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A New Algorithm for Deduction of Hepatitis B Surface Antigen Subtype Determinants from the Amino Acid Sequence

Cited by 73 publications

References 37 publications

Hepatitis B Virus Infection in Indonesia 15 Years After Adoption of a Universal Infant Vaccination Program: Possible Impacts of Low Birth Dose Coverage and a Vaccine-Escape Mutant

Hepatitis B Virus Infection in Indonesia 15 Years After Adoption of a Universal Infant Vaccination Program: Possible Impacts of Low Birth Dose Coverage and a Vaccine-Escape Mutant

Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Pre-Operative Surgical Patients; Serological Screening of Hepatitis B, Its Knowledge and Attitudes in Quetta, Pakistan

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