A neuronal wiring platform through microridges for rationally engineered neural circuits

Wu, Yu; Wang, Meijian; Wang, Yong; Yang, Huiran; Qi, Hao; Seicol, Benjamin J; Xie, Ruili; Guo, Liang

doi:10.1063/5.0025921

Cited by 6 publications

(4 citation statements)

References 58 publications

(14 reference statements)

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“…Organizing cells in a controlled manner is of great importance for tissue engineering and future therapies. Moreover, engineering neuronal networks is a fundamental step towards artificial brain organoids on chips, bio-hybrid implantations, or engineered neuronal circuits for bio-electronic computational devices [ 16 , 27 – 29 ]. The development of different fabricated magnetic shapes can be used for various applications leading to controllable networks.…”

Section: Discussionmentioning

confidence: 99%

A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance

et al. 2023

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The ability to control neuronal mobility and organization is of great importance in developing neuronal interfaces and novel therapeutic approaches. An emerging promising method is the manipulation of neuronal cells from afar via magnetic forces. Nevertheless, using magnetic iron oxide nanoparticles as internal actuators may lead to biotoxicity, adverse influence on intracellular processes, and thus requires prerequisite considerations for therapeutic approaches. Magnetizing the cells via the incorporation of magnetic particles that can be applied extracellularly is advantageous. Herein, we have developed a magnetic system based on streptavidin–biotin interaction to decorate cellular membrane with magnetic elements. In this model, superparamagnetic microparticles, coated with streptavidin, were specifically bound to biotinylated PC12 cells. We demonstrated that cell movement can be directed remotely by the forces produced by pre-designed magnetic fields. First, using time lapse imaging, we analyzed the kinetics of cell migration towards the higher flux zone. Next, to form organized networks of cells we designed and fabricated micro-patterned magnetic devices. The fabricated devices were composed of a variety of ferromagnetic shapes, sputter-deposited onto glass substrates. Cells that were conjugated to the magnetic particles were plated atop the micro-patterned substrates, attracted to the magnetic actuators and became fixed onto the magnetic patterns. In all, our study presents a novel system based on a well-known molecular technology combined with nanotechnology that may well lead to the expansion of implantable magnetic actuators to organize and direct cellular growth.

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Section: Discussionmentioning

confidence: 99%

A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance

et al. 2023

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“…However, the elimination of key elements can contribute to the suppression of emergent or synergistic functions and, therefore, to spurious conclusions. Thus, complexity must be considered at scale, depending whether the research question may be better addressed by low-complexity models that simulate simple circuits 71 or high-complexity alternatives that simulate networks and systems 72 , 73 .…”

Section: Design Considerations Of Cns Modelsmentioning

confidence: 99%

Functional bioengineered models of the central nervous system

2023

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The functional complexity of the central nervous system (CNS) is unparalleled in living organisms. Its nested cells, circuits and networks encode memories, move bodies and generate experiences. Neural tissues can be engineered to assemble model systems that recapitulate essential features of the CNS and to investigate neurodevelopment, delineate pathophysiology, improve regeneration and accelerate drug discovery. In this Review, we discuss essential structure–function relationships of the CNS and examine materials and design considerations, including composition, scale, complexity and maturation, of cell biology-based and engineering-based CNS models. We highlight region-specific CNS models that can emulate functions of the cerebral cortex, hippocampus, spinal cord, neural-X interfaces and other regions, and investigate a range of applications for CNS models, including fundamental and clinical research. We conclude with an outlook to future possibilities of CNS models, highlighting the engineering challenges that remain to be overcome.

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“…Even though uoride has long been thought to be a developmental neurotoxin, the mechanisms behind uoride's adverse effects on the developing brain are unknown. Hundreds of billions of synapses of individual brain neurons are connected to create precise neural circuits that support cognitive and peak performance (Wu Y et al, 2020). Cognitive di culties and mental retardation are neurological developmental problems caused by inadequate synapse formation or function throughout infancy (Lepeta K et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Fluoride – influenced presynaptic and MAPK – mediated TrkB signaling alterations, induced neurotoxicity

Gopalan¹,

Venkatramanan²

2023

Preprint

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Fluoride (F), an inorganic substance, is everywhere in the environment. Fluoride exposure is caused mainly by geogenic sources, such as drinking wells and deep soil water. Excess fluoride-containing water usage for many years may cause neurotoxic damage. The mechanism underlying the neurotoxicology of endemic fluorosis remains obscure. Fluoride crosses the blood-brain barrier and accumulates within neurons. In our study, chronic exposure to fluoride-induced epileptogenesis in neuroblastoma (SK-N-SH) cells was followed with tools like Brain-derived neurotrophic factor (BDNF) and Synapsin 1 (SYN 1) protein expression. SK-N-SH cells were incubated with fluoride water (1 mg/L and 6 mg/L) for 24 h in-vitro. Prolonged ingestion of fluoride causes chronic fluorosis. Fluoride exposure reduced cell viability gradually from 48 to 96 h. The standard structure of axon spines and dendritic outgrowth in high fluoride water vanished. TrkB activated MAPK/ERK downstream signaling pathway is triggered by increased BDNF protein expression and decreased SYN 1 protein levels observed in fluoride water incubation. Further 96 hours incubation with fluoride-cleared water increased viability of SK-N-SH cells and a normal expression of BDNF and SYN 1 protein levels, suggests that fluoride-blocked BDNF-TrkB pathway might have been replaced by high-levels expression of BDNF protein where incubation medium contains concomitant lowered levels of fluoride. Hypothesis is that scaffold proteins regained normal expression might have influenced conducted normal excitation and transmission could reduce neurotoxic effect or even might control seizures.

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A neuronal wiring platform through microridges for rationally engineered neural circuits

Cited by 6 publications

References 58 publications

A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance

A streptavidin–biotin system combined with magnetic actuators for remote neuronal guidance

Functional bioengineered models of the central nervous system

Fluoride – influenced presynaptic and MAPK – mediated TrkB signaling alterations, induced neurotoxicity

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