“…While we chose to photoadduct in the rostral pons as proof of principle in studying systemic effects of local CNS anesthetic photolabel adduction, this same approach could be extended to multiple other brain regions previously shown to be involved in arousal and as targets for anesthetic effects. Numerous and disparately located CNS centers, including other areas of the pons, medulla, midbrain, hypothalamus, basal forebrain, thalamus, and cortex, have been demonstrated to be critical for anesthetic action or emergence from anesthesia ( Devor and Zalkind, 2001 ; Abulafia et al, 2009 ; Luo and Leung, 2009 ; Moore et al, 2012 ; Solt et al, 2014 ; Z. Zhang et al, 2015 ; Gelegen et al, 2018 ; Pal et al, 2018 ; Gao et al, 2019 ; Wang et al, 2019 ; J. Y. Li et al, 2022 ). Our results confirm that in vivo photoadduction produces local therapeutic-level concentrations of drug in ROIs.…”