A nested PCR-RFLP method for high-resolution genotyping of HLA-B alleles

篤子, 小川; 滋樹, 光永; 勝士, 徳永; 善英, 石川; 達也, 赤座; 憲治, 田所; 猛夫, 十字

doi:10.12667/mhc.5.4

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Genomic DNAs from eight HLA‐B48‐homozygous healthy individuals of Japanese origin were prepared to investigate MICA and MICB alleles. All of them have HLA‐B*4801 in a homozygous state, as assessed by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) ( 16). MICA and MICB alleles were determined by PCR‐based direct sequencing, as previously described ( 14, 15).…”

Section: Hla Microsatellite and Sts Markers In B48 Homozygous Japanesementioning

confidence: 99%

On the MICA deleted‐MICB null, HLA‐B*4801 haplotype

Ôta

Bahram²,

Katsuyama

et al. 2000

Tissue Antigens

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A 100-kb deletion including the MICA gene was recently reported in the HLA-B48 (B*4801)-associated haplotype in Japanese. Interestingly, this MICA deletion is accompanied by a MICB null allele, MICB0107N. In order to further investigate the universality of the apparent tight linkage between these two events, we present data on high-resolution deletion mapping of eight HLA-B48-homozygous individuals. Among these, five carried the MICA deletion linked to MICB0107N, as originally reported. Conversely, the remaining three possessed an intact MICA gene of MICA008 or MICA010 allelic variant associated this time with a putative expressed MICB allele, MICB0102. These results may imply that the expression of both MICA and MICB molecules is indispensable to viability through a yet-to-be understood mutual interaction in immune surveillance.

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Section: Hla Microsatellite and Sts Markers In B48 Homozygous Japanesementioning

confidence: 99%

On the MICA deleted‐MICB null, HLA‐B*4801 haplotype

Ôta

Bahram²,

Katsuyama

et al. 2000

Tissue Antigens

View full text Add to dashboard Cite

show abstract

“…HLA‐A alleles were determined with the PCR‐RFLP method combined with allele‐, group‐, and sequence‐specific amplification 19,20 . HLA‐B alleles were mainly typed by the PCR‐RFLP method 21 . As a supplementary method, PCR with sequence‐specific primers (PCR‐SSP) was also employed 22 .…”

Section: Methodsmentioning

confidence: 99%