A nested PCR for the ssrRNA gene detects Trypanosoma binneyi in the platypus and Trypanosoma sp. in wombats and kangaroos in Australia1

Noyes, Harry; Stevens, Jamie R.; Teixeira, Magaly Gêmio; Phelan, J; Holz, Peter

doi:10.1016/s0020-7519(98)00167-2

Cited by 158 publications

(189 citation statements)

References 19 publications

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“…However, the differences between these and the present results may largely be due to differences in diagnostic methods. Traditionally, detection of trypanosomes in field samples has been based on microscopic observations of Giemsa-stained thin and thick blood smears or culture (Hoare, 1972 ;Noyes et al 1999). The sensitivity of PCR-based methods is believed to be 2-3 times higher (Masiga et al 1992 ;Desquesnes and Davila, 2002), which is borne out by the survey of Solano et al (1999) of a cattle herd in Burkina Faso (microscopy 5 .…”

Section: Discussionmentioning

confidence: 99%

Trypanosomes, fleas and field voles: ecological dynamics of a host-vector–parasite interaction

et al. 2005

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To investigate the prevalence of a flea-borne protozoan (Trypanosoma (Herpetosoma) microti) in its field vole (Microtus agrestis) host, we monitored over a 2-year period a range of intrinsic and extrinsic parameters pertaining to host demographics, infection status and vector (flea) prevalence. Generalized Linear Mixed Modelling was used to analyse patterns of both flea and trypanosome occurrence. Overall, males of all sizes and ages were more likely to be infested with fleas than their female counterparts. Flea prevalence also showed direct density dependence during the winter, but patterns of density dependence varied amongst body mass (age) classes during the summer. Trypanosome prevalence did not vary between the sexes but was positively related to past flea prevalence with a lag of 3 months, with the highest levels occurring during the autumn season. A convex age-prevalence distribution was observed, suggesting that individuals develop a degree of immunity to trypanosome infection with age and exposure. An interaction between age and whether the individual was new or recaptured suggested that infected animals are less likely to become territory holders than their uninfected counterparts.

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Section: Discussionmentioning

confidence: 99%

Trypanosomes, fleas and field voles: ecological dynamics of a host-vector–parasite interaction

et al. 2005

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“…One isolate, Q4112-4117 from TPB, which grouped with T. copemani genotype B clade at the 18S rRNA locus, grouped by itself but close to a loose clade that included G8 from a woylie (KC812988) [8], H25 from a kangaroo (AJ620276) and sequences from brushtail possums (Trichosurus vulpecula) (JN315395 and JN315396) [1,7,12]. This isolate (Q4112-4117) exhibited 3.9% genetic distance from H25 and 1.8% genetic distance from brushtail possums (JN315395 and JN315396).…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…The phylogenetic relationship of T. copemani to other trypanosome species at the 18S rRNA ( [8] and also grouped with sequences from wombats (AA1-AJ620559, H26-AJ009169 and APP-AJ620558) [1].…”

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confidence: 98%

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Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia

Austen

Paparini

Reid

et al. 2016

Parasitology International

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“…DNA was extracted from lter papers using a modi ed PurGene® (Qiagen, Canada) extraction procedure for compromised blood samples. Primers described by Noyes et al (1999) were used to test for the presence of Trypanosoma and the primers described by Laaksonen et al (2009) were used to test for the presence of Setaria. Unfortunately, DNA quality was variable and DNA was not reliably extracted from the lter papers (D. Schock & S. Kutz, unpubl.…”

Section: Level 2 Sampling (N = 166 Animals)mentioning

confidence: 99%

Standardized monitoring of Rangifer health during International Polar Year

Kutz¹,

Ducrocq²,

Cuyler³

et al. 2013

Ran

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Monitoring of individual animal health indices in wildlife populations can be a powerful tool for evaluation of population health, detecting changes, and informing management decisions. Standardized monitoring allows robust comparisons within and across populations, and over time and vast geographic regions. As an International Polar Year Initiative, the CircumArctic Rangifer Monitoring and Assessment network established eld protocols for standardized monitoring of caribou and reindeer (Rangifer tarandus) health, which included body condition, contaminants, and pathogen exposure and abundance. To facilitate use of the protocols, training sessions were held, additional resources were developed, and language was translated where needed. From March 2007 to September 2010, at least 1206 animals from 16 circumpolar herds were sampled in the eld using the protocols. Four main levels of sampling were done and ranged from basic to comprehensive sampling. Possible sources of sampling error were noted by network members early in the process and protocols were modi ed or supplemented with additional visual resources to improve clarity when needed. is is the rst time that such broad and comprehensive circumpolar sampling of migratory caribou and wild reindeer, using standardized protocols covering both body condition and disease status, has been done.

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A nested PCR for the ssrRNA gene detects Trypanosoma binneyi in the platypus and Trypanosoma sp. in wombats and kangaroos in Australia1

Cited by 158 publications

References 19 publications

Trypanosomes, fleas and field voles: ecological dynamics of a host-vector–parasite interaction

Trypanosomes, fleas and field voles: ecological dynamics of a host-vector–parasite interaction

Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia

Standardized monitoring of Rangifer health during International Polar Year

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