A Nephrostomy-associated Urinary Tract Infection Caused by &lt;i&gt;Elizabethkingia meningoseptica&lt;/i&gt;

Hagiya, Hideharu; Ogawa, Hiroyasu; Takahashi, Yusuke; Hasegawa, Kou; Iwamuro, Masaya; Otsuka, Fumio

doi:10.2169/internalmedicine.54.4998

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…(Han and Andrade, 2005 ), Elizabethkingia spp. (Zong, 2014 ; Hagiya et al, 2015 ), and Methylobacterium spp. (Lee et al, 2004 ) that are increased in UUI have been associated previously with urinary tract infections.…”

Section: Resultsmentioning

confidence: 99%

Does the Urinary Microbiome Play a Role in Urgency Urinary Incontinence and Its Severity?

Karstens

Asquith

Davin

et al. 2016

Front. Cell. Infect. Microbiol.

215

202

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Objectives: Traditionally, the urinary tract has been thought to be sterile in the absence of a clinically identifiable infection. However, recent evidence suggests that the urinary tract harbors a variety of bacterial species, known collectively as the urinary microbiome, even when clinical cultures are negative. Whether these bacteria promote urinary health or contribute to urinary tract disease remains unknown. Emerging evidence indicates that a shift in the urinary microbiome may play an important role in urgency urinary incontinence (UUI). The goal of this prospective pilot study was to determine how the urinary microbiome is different between women with and without UUI. We also sought to identify if characteristics of the urinary microbiome are associated with UUI severity.Methods: We collected urine from clinically well-characterized women with UUI (n = 10) and normal bladder function (n = 10) using a transurethral catheter to avoid bacterial contamination from external tissue. To characterize the resident microbial community, we amplified the bacterial 16S rRNA gene by PCR and performed sequencing using Illumina MiSeq. Sequences were processed using the workflow package QIIME. We identified bacteria that had differential relative abundance between UUI and controls using DESeq2 to fit generalized linear models based on the negative binomial distribution. We also identified relationships between the diversity of the urinary microbiome and severity of UUI symptoms with Pearson's correlation coefficient.Results: We successfully extracted and sequenced bacterial DNA from 95% of the urine samples and identified that there is a polymicrobial community in the female bladder in both healthy controls and women with UUI. We found the relative abundance of 14 bacteria significantly differed between control and UUI samples. Furthermore, we established that an increase in UUI symptom severity is associated with a decrease in microbial diversity in women with UUI.Conclusions: Our study provides further characterization of the urinary microbiome in both healthy controls and extensively phenotyped women with UUI. Our results also suggest that the urinary microbiome may play an important role in the pathophysiology of UUI and that the loss of microbial diversity may be associated with clinical severity.

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Section: Resultsmentioning

confidence: 99%

Does the Urinary Microbiome Play a Role in Urgency Urinary Incontinence and Its Severity?

Karstens

Asquith

Davin

et al. 2016

Front. Cell. Infect. Microbiol.

215

202

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“…18,24 Penggunaan nefrostomi juga dapat meningkatkan risiko infeksi. 25 Pada pasien dengan infeksi pasca operasi diberikan terapi simptomatik, bila tidak ada perbaikan, dilakukan pemeriksaan kultur darah atau kultur urine. Umumnya hasil kultur didapatkan dalam 5-7 hari, selama menunggu kultur diberikan antibiotik generasi lebih tinggi dibanding antibiotik awal.…”

Section: Pembahasanunclassified

Perbandingan Mikroorganisme Pada Kultur Urin Pre-Operasi Dan Kultur Batu Pasca Operasi Pada Pasien Batu Saluran Kemih

Jaya

Prihartomo

Afaf

2023

JIK

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Urinary tract infection (UTI) and urinary tract calculi (UTC) still causes significant health problem and present as a dominant case in urology. Urine culture is a standard examination prior to UTC surgery which provide information on microorganism and its sensitivity to antibiotics. Despite of appropriate preoperative antibiotic therapy, we still found post operative infections among those patients. This study aims to determine if there any difference between urine and calculi culture in patients underwent UTC surgery. This is a comparative observational study in patients underwent urinary stone removal procedures from April 2022 to August 2022. Urinary culture attained 5-7 days prior to procedure and crushed calculi culture directly after procedure. The comparison between two examinations was performed. Total of 50 cases consisting of 33 (66%) males and 17 (34%) females was included. Patient with positive urine culture and calculi culture was 15 (30%) and 14 (28%) respectively. Only 3 (6%) patients positive for both, and only one patient with same pathogen. In 11 (22%) patients, we found positive calculi culture with previously sterile urine culture. Most common pathogen in urine culture was Escherichia coli, and Klebsiella pneumoniae found mostly in calculi culture. This study showed 21% accuracy, 66,67% specificity, 20% positive prediction value, 68,57% negative prediction value and 54% diagnostic accuracy. Low positive prediction value and diagnostic accuracy for microorganism of preoperative urine cultured was shown in this study. As a consequence, calculi culture is required as a routine examination during stone removal procedures.

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“…and Methylobacterium spp. [ 52 , 53 , 54 , 55 , 56 ]) were also detected in increased abundance in women with UUI [ 49 ], suggesting a persistent low-grade infection of bacteria being responsible for the irritative symptoms of UUI, at least for some individuals.…”

Section: The Bladder Microbiome and Urological Disordersmentioning

confidence: 99%

Bladder Microbiome in the Context of Urological Disorders—Is There a Biomarker Potential for Interstitial Cystitis?

Bschleipfer

Karl

2022

Diagnostics

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Since the development of modern cultivation and sequencing techniques, the human microbiome has increasingly become the focus of scientific attention. Even in the bladder, long considered to be a sterile niche, a highly variable and complex microbial colonization has now been demonstrated. Especially in the context of diseases such as interstitial cystitis, whose etiopathogenesis is largely unknown, and whose diagnosis is based on a process of exclusion of confusable diseases, science hopes to gain far-reaching insights for etiology and diagnosis, including the identification of potential biomarkers. While for functional disorders such as urge urinary incontinence and overactive bladder syndrome, initial associations have been demonstrated between reduced microbial diversity and increased symptomatology, as well as shifts in the abundance of specific microorganisms such as Lactobacillus or Proteus, studies in interstitial cystitis show conflicting results and have failed to identify a putative organism or urotype that clearly distinguishes the urinary microbiome of patients with IC/BPS from that of healthy controls. At the present time, therefore, the new insights into the bladder microbiome and its potential influence on urologic disease cannot yet be used in the context of elucidating possible etiopathogenetic causes, as well as in the use of a biomarker for diagnostic or prognostic purposes. Further studies should focus primarily on uniform procedures and detection methods to achieve better comparability of results and increase the likelihood of detecting hidden patterns.

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A Nephrostomy-associated Urinary Tract Infection Caused by <i>Elizabethkingia meningoseptica</i>

Cited by 8 publications

References 15 publications

Does the Urinary Microbiome Play a Role in Urgency Urinary Incontinence and Its Severity?

Does the Urinary Microbiome Play a Role in Urgency Urinary Incontinence and Its Severity?

Perbandingan Mikroorganisme Pada Kultur Urin Pre-Operasi Dan Kultur Batu Pasca Operasi Pada Pasien Batu Saluran Kemih

Bladder Microbiome in the Context of Urological Disorders—Is There a Biomarker Potential for Interstitial Cystitis?

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