A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Hecker, Yanina P.; Cóceres, Verónica M.; Wilkowsky, Silvina Elizabeth; Ortiz, José Manuel Jaramillo; Morrell, Eleonora; Verna, Andrea Elizabeth; Ganuza, Agustina; Cano, D.; Lischinsky, L.; Angel, Sérgio O.; Zamorano, Patricia; Odeón, Anselmo Carlos; Leunda, María; Campero, Carlos Manuel; Morein, Brør; Moore, D.P.

doi:10.1016/j.vetimm.2014.11.001

Cited by 17 publications

(18 citation statements)

References 58 publications

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“…In fact, Pinitkiatisakul et al (71) demonstrated that s.c. immunization with recombinant protein SRS2 from N. caninum (rNcSRS2) + ISCOMs diminished the clinical symptoms of the disease in a mouse model of cerebral neosporosis. However, s.c. immunizations with vaccine formulations containing ISCOMs as adjuvants and native antigen extracts (67, 68) or the combination of recombinant N. caninum proteins (66) in the target species, Bos taurus , induced high titers of IgGs (66–68) and similar levels of IFN-γ to those achieved after inoculation with live NC-1 (68), but failed to prevent vertical transmission (66, 67). Similarly, examples of ISCOMs vaccine formulations are found against T. gondii infection in s.c. immunization protocols in pigs using as antigens total native antigens from T. gondii (73) or crude ropthry proteins (72), also depicting high humoral (72, 73) and cellular immune responses (73) but only partial protection against infection (87).…”

Section: Adjuvants: the Black Box Of Immunology Being Openedmentioning

confidence: 99%

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

2019

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Coccidial parasites cause medical and veterinary diseases worldwide, frequently leading to severe illness and important economic losses. At present, drugs, chemotherapeutics and prophylactic vaccines are still missing for most of the coccidial infections. Moreover, the development and administration of drugs and chemotherapeutics against these diseases would not be adequate in livestock, since they may generate unacceptable residues in milk and meat that would avoid their commercialization. In this scenario, prophylactic vaccines emerge as the most suitable approach. Subunit vaccines have proven to be biologically safe and economically viable, allowing researchers to choose among the best antigens against each pathogen. However, they are generally poorly immunogenic and require the addition of adjuvant compounds to the vaccine formulation. During the last decades, research involving plant immunomodulatory compounds has become an important field of study based on their potential pharmaceutical applications. Some plant molecules such as saponins, polysaccharides, lectins and heat shock proteins are being explored as candidates for adjuvant/carriers formulations. Moreover, plant-derived immune stimulatory compounds open the possibility to attain the main goal in adjuvant research: a safe and non-toxic adjuvant capable of strongly boosting and directing immune responses that could be incorporated into different vaccine formulations, including mucosal vaccines. Here, we review the immunomodulatory properties of several plant molecules and discuss their application and future perspective as adjuvants in the development of vaccines against coccidial infections.

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Section: Adjuvants: the Black Box Of Immunology Being Openedmentioning

confidence: 99%

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

2019

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“…The bacterially expressed and purified recombinant antigens rNcSAG1, rNcHSP20 and rNcGRA7 were formulated with ISCOMs and inoculated into heifers prior to mating. Immunogens were sc administered twice, with one group receiving all three antigens, one receiving ISCOMs only, and one receiving sterile phosphate‐buffered saline (PBS) only . Challenge was done at day 70 of gestation employing the Nc‐1 isolate.…”

Section: Vaccine Development: Present Statusmentioning

confidence: 99%

Vaccines for bovine neosporosis: current status and key aspects for development

Horcajo

Regidor‐Cerrillo

Aguado‐Martínez

et al. 2016

Parasite Immunology

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SUMMARYBovine neosporosis is a worldwide concern due to its global distribution and great economic impact. Reproductive failure in cattle due to abortion leads to major economic losses associated with the disease. Currently, there is no treatment or vaccine available against abortion or transmission caused by Neospora caninum infection in cattle. However, vaccination is considered the best measure of control against bovine neosporosis. Several host and parasite factors can influence the dynamics of the infection in bovines. Moreover, the availability of well-defined infection models is a key factor for the evaluation of vaccine candidates. However, working with cattle is not easy due to difficult handling, facilities and costs, and therefore, 'more affordable' models could be used for screening of promising vaccines to establish proof of concept. So far, live-attenuated vaccines have shown good efficacy against exogenous transplacental transmission; however, they have relevant disadvantages and associated risks, which render inactivated or subunit vaccines the best way forward. The identification of novel potential targets and vaccines, and the application of innovative vaccine technologies in harmonized experimental animal models, will accelerate the development of an effective vaccine against bovine neosporosis.

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“…NcSpain1H and NcNowra) and then challenged during defined time points of gestation demonstrated substantial protection against abortion and fetal loss (Williams et al 2007; Rojo-Montejo et al 2013; Weber et al 2013). Vaccination of pregnant cattle with tachyzoites of a live-attenuated vaccine strain (Hecker et al 2013), iscom preparations of native N. caninum extract (Hecker et al 2013), or recombinant antigens (SAG1, Hsp20, GRA7) incorporated into iscoms (Hecker et al 2014) demonstrated partial protection against transplacental fetal infection with the live vaccine, but not with the native or recombinant subunit vaccines. In addition, a live vaccine isolate (NcIs491) was assessed in a field trial comprised of 520 pregnant and N. caninum seropositive cows, of which 146 were vaccinated at mid-gestation and 374 served as controls (Mazuz et al 2015).…”

Section: Vaccines Against Neosporosismentioning

confidence: 99%

Approaches for the vaccination and treatment ofNeospora caninuminfections in mice and ruminant models

2015

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Neospora caninum is a leading cause of abortion in cattle, and is thus an important veterinary health problem of high economic significance. Vaccination has been considered a viable strategy to prevent bovine neosporosis. Different approaches have been investigated, and to date the most promising results have been achieved with live-attenuated vaccines. Subunit vaccines have also been studied, and most of them represented components that are functionally involved in (i) the physical interaction between the parasite and its host cell during invasion or (ii) tachyzoite-to-bradyzoite stage conversion. Drugs have been considered as an option to limit the effects of vertical transmission of N. caninum. Promising results with a small panel of compounds in small laboratory animal models indicate the potential value of a chemotherapeutical approach for the prevention of neosporosis in ruminants. For both, vaccines and drugs, the key for success in preventing vertical transmission lies in the application of bioactive compounds that limit parasite proliferation and dissemination, without endangering the developing fetus not only during an exogenous acute infection but also during recrudescence of a chronic infection. In this review, the current status of vaccine and drug development is presented and novel strategies against neosporosis are discussed.

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A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Cited by 17 publications

References 58 publications

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

Promising Plant-Derived Adjuvants in the Development of Coccidial Vaccines

Vaccines for bovine neosporosis: current status and key aspects for development

Approaches for the vaccination and treatment ofNeospora caninuminfections in mice and ruminant models

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