A neonatal hypocalcemia due to maternal vitamin D deficiency. Reviewing supplementation

Lanceta, Víctor Adán; Ruiz, Nuria Martín; Costey, Silvia Benito; Lorente, Roberto Alijarde; Fernández, José Miguel Martínez de Zabarte

doi:10.1016/j.anpede.2020.09.015

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Glial cell monocultures or cocultures could be also used to mimic different postnatal conditions, like for instance hypernatremia, hyperchloremia, hypo/hypercalcemia, etc. [ 94 , 95 ]. The neonatal glial cultures are also useful for studies on neurodegenerative diseases, including PD [ 96 , 97 ], AD [ 98 , 99 , 100 , 101 ], MS [ 102 , 103 ], or triggered by exposure to environmental toxicants and pollution [ 104 , 105 , 106 ].…”

Section: Discussionmentioning

confidence: 99%

Neonatal Rat Glia Cultured in Physiological Normoxia for Modeling Neuropathological Conditions In Vitro

Gargas

Janowska

Ziabska

et al. 2022

IJMS

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Cell culture conditions were proven to highly affect crucial biological processes like proliferation, differentiation, intercellular crosstalk, and senescence. Oxygen tension is one of the major factors influencing cell metabolism and thus, modulating cellular response to pathophysiological conditions. In this context, the presented study aimed at the development of a protocol for efficient culture of rat neonatal glial cells (microglia, astrocytes, and oligodendrocytes) in oxygen concentrations relevant to the nervous tissue. The protocol allows for obtaining three major cell populations, which play crucial roles in sustaining tissue homeostasis and are known to be activated in response to a wide spectrum of external stimuli. The cells are cultured in media without supplement addition to avoid potential modulation of cell processes. The application of active biomolecules for coating culturing surfaces might be useful for mirroring physiological cell interactions with extracellular matrix components. The cell fractions can be assembled as cocultures to further evaluate investigated mechanisms, intercellular crosstalk, or cell response to tested pharmacological compounds. Applying additional procedures, like transient oxygen and glucose deprivation, allows to mimic in vitro the selected pathophysiological conditions. The presented culture system for neonatal rat glial cells is a highly useful tool for in vitro modeling selected neuropathological conditions.

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Section: Discussionmentioning

confidence: 99%

Neonatal Rat Glia Cultured in Physiological Normoxia for Modeling Neuropathological Conditions In Vitro

Gargas

Janowska

Ziabska

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Clinical studies have shown that vitamin D supplementation can lower androgen levels, lower anti-Mullerian hormone (AMH) levels [ 14 , 15 ], normalize the metabolic profile, and regularize periods in women with polycystic ovarian syndrome (PCOS) [ 16 , 17 ]. In fact, low serum 25(OH)D concentrations have been linked to problems of infertility [ 18 , 19 ], endometriosis [ 20 ], polycystic ovary syndrome (PCOS) [ 16 , 17 , 21 , 22 ], as well as adverse pregnancy outcomes, including spontaneous abortions [ 23 ], gestational diabetes [ 24 , 25 ], bacterial vaginosis [ 26 ], preeclampsia [ 27 , 28 ], neonatal hypocalcemia [ 29 ], prematurity, and low birth weight [ 30 ], as well as fetal and infant growth disorders [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Sun Exposure Score and Vitamin D Levels in Moroccan Women of Childbearing Age

et al. 2023

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Sunlight exposure is an essential source of vitamin D for many humans. However, hypovitaminosis D is a global public health problem. This study aimed to develop and validate a sun exposure score (SES) and correlate it with serum 25-hydroxyvitamin D levels in women of childbearing age. One hundred and sixty women aged 18 to 45 years residing in Meknes, Morocco, were included. A questionnaire estimating the sun exposure score and blood analysis of serum 25-OHD concentration were performed. The questionnaire’s reliability and construct validity were evaluated using Cronbach’s alpha and factor analysis. Spearman’s test was used to assess the correlation between SES and 25-OHD levels. The score’s reliability and construct validity were good, with Cronbach’s alpha values >0.70 and factorial saturation ranging from 0.696 to 0.948. Serum 25-OHD levels were significantly associated with the total sun exposure score, and all SES domains (Rho was 0.615 (p < 0.0001), 0.307 (p < 0.0001), 0.605 (p < 0.0001), and 0.424 (p < 0.0001) for total SES, indoor exposure domain, outdoor exposure domain, and sun protection practice domain, respectively). In addition, median 25-OHD levels increased significantly when sun exposure was changed from insufficient to sufficient (p < 0.0001). The results suggest that the sun exposure score could be used as a clinical tool to assess vitamin D levels in women of childbearing age.

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A neonatal hypocalcemia due to maternal vitamin D deficiency. Reviewing supplementation

Cited by 2 publications

References 5 publications

Neonatal Rat Glia Cultured in Physiological Normoxia for Modeling Neuropathological Conditions In Vitro

Neonatal Rat Glia Cultured in Physiological Normoxia for Modeling Neuropathological Conditions In Vitro

Sun Exposure Score and Vitamin D Levels in Moroccan Women of Childbearing Age

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