A neolignan and lignans from Magnolia biondii

Structures and Inhibitory Activities for Interleukin-2 Production of Seasonally Variable Constituents in Flower Parts of <i>Magnolia kobus</i> at Different Growth Stages

“…Tiliroside (1): 32) Syringin (2): 33) IL-2 and IL-6 Inhibitory Activities BALB/c mice were treated and handled according to the Tokyo University of Science's institutional ethical guidelines for animal experiments and with the approval of Tokyo University of Science's Institutional Animal Care and Use Committee (permit number S18007) as described previously. 24,33) We prepared culture supernatants of mouse splenic lymphocytes and measured the concentrations of IL-2 and IL-6 using enzyme-linked immunosorbent assay (ELISA) as described previously. 7,34) Measurement of IL-2 mRNA Expression We measured the IL-2 mRNA expression using real time RT-PCR as described previously.…”

“…Several active constituents exhibiting various biological activities from Magnolia Flower have been identified, according to the literature, including lignans with IL-2 inhibitory 19) and cholesterol-lowering 20) activity, neolignans with antiinflammatory 21) and anti-vasculogenesis 22) activity, and volatile oils with anti-inflammatory activity. 23) There have also been many reports on the isolation of volatile oils, 24) lignans, 25) neolignans, 25) phenylpropanoids, 26) alkaloids, 27) monoterpene glycosides, 28) and flavonoids 15) from Magnolia species in efforts to study biological activity.…”

“…Previous studies have reported that variations in the volatile oils of the buds of Magnolia species were influenced by seasonal changes, 24) differences in habitats, 29) and botanical origins. 30) However, the relationship between chemical compositions and pharmacological effects of Magnolia Flower based on seasonal variation has not been examined and the biologically active constituents influenced by seasonal change have also not been identified.…”

Four New Lignans and IL-2 Inhibitors from Magnoliae Flos

“…Quantitative PCR and Real-Time PCR Jurkat T cells (1×10 6 ) were incubated with indicated concentrations of compounds for 30 min at 37°C. Incubated cells were stimulated with PMA (200 nM) and A23187 (1 µM) for 6 h for PCR.…”

“…The dried flower buds of M. biondii have historically been used for the symptomatic management of allergic rhinitis, sinusitis, and headache in Chinese medicine. 4) Previous phytochemical investigations of this species revealed that it is a valuable natural source of essential oils, 5) lignans, 6,7) neolignans, 6) sesquiterpenes, 8) flavonoids, 9) and alkaloids, 10) which have biological activities such as anti-platelet-activating factor (anti-PAF), 11) anti-inflammatory, 12) anti-allergenic, 9) and anti-angiogenic activities, 13) cholesterol acyltransferase (ACA T) inhibitory activity, 14) proapoptotic activity, 15) and vasorelaxant effects. 16) In recent research, lignans from M. biondii were demonstrated to possess anti-allergenic activity in vitro.…”