A Nematode Larval Motility Inhibition Assay for Screening Plant Extracts and Natural Products

Lorimer, Stephen D.; Perry, Nigel B.; Foster, Lysa M.; Burgess, Elaine J.; Douch, P.G.C.; Hamilton, Mary C.; Donaghy, Morgan J.; Mcgregor, R.

doi:10.1021/jf9602176

Cited by 61 publications

(44 citation statements)

References 17 publications

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“…It has been shown to be effective against H. contours and T. colubriformis (Bennet-Jenkins and Bryant, 1996;Lorimer et al, 1996). These effects are attributed to the presence of tannins/ polyphenols in eucalyptus.…”

Section: Plants Containing Anthelmintic Compoundsmentioning

confidence: 99%

Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems

Makkar

Francis

Becker

2007

Animal

370

214

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Livestock and aquaculture production is under political and social pressure, especially in the European Union (EU), to decrease pollution and environmental damage arising due to animal agriculture. The EU has banned the use of antibiotics and other chemicals, which have been shown to be effective in promoting growth and reducing environment pollutants because of the risk caused to humans by chemical residues in food and by antibiotic resistance being passed on to human pathogens. As a result of this, scientists have intensified efforts in exploiting plants, plant extracts or natural plant compounds as potential natural alternatives for enhancing the livestock productivity. This paper discusses work on the effects of various phytochemicals and plant secondary metabolites in ruminant and fish species. The focus is on (i) plants such as Ananas comosus (pine apple), Momordica charantia (bitter gourd) and Azadirachta indica (neem) containing anthelmintic compounds and for their use for controlling internal parasites; (ii) plants containing polyphenols and their applications for protecting proteins from degradation in the rumen, increasing efficiency of microbial protein synthesis in rumen and decreasing methane emission; for using as antioxidants, antibacterial and antihelmintic agents; and for changing meat colour and for increasing n-3 fatty acids and conjugated linoleic acid in meat; (iii) saponin-rich plants such as quillaja, yucca and Sapindus saponaria for increasing the efficiency of rumen fermentation, decreasing methane emission and enhancing growth; for producing desired nutritional attributes such as lowering of cholesterol in monogastric animals; for increasing growth of fish (common carp and Nile tilapia) and for changing male to female ratio in tilapia; and for use as molluscicidal agents; (iv) Moringa oleifera leaves as a source of plant growth factor(s), antioxidants, beta-carotene, vitamin C, and various glucosinolates and their degraded products for possible use as antibacterial, antioxidant, anticarcinogenic and antipest agents; (v) Jatropha curcas toxic variety with high levels of various phytochemicals such as trypsin inhibitor, lectin, phytate and phorbol esters in seeds limiting the use of seed meal in fish and livestock diets; and the use of phorbol esters as bio-pesticidal agent; and (vi) lesser-known legumes such as Entada phaseoloides seeds containing high levels of trypsin inhibitor and saponins, Sesbania aculeate seeds rich in non-starch polysaccharides and Mucuna pruriens var. utilis seeds rich in L-3,4-dihydroxyphenylalanine and their potential as fish feed; Cassia fistula seeds as a source of antioxidants; and the use of Canavalia ensiformis, C. gladiata and C. virosa seeds containing high levels of trypsin inhinitor, lectins and canavanine. The paper also presents some challenges and future areas of work in this field.

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Section: Plants Containing Anthelmintic Compoundsmentioning

confidence: 99%

Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems

Makkar

Francis

Becker

2007

Animal

370

214

View full text Add to dashboard Cite

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“…Two-fold dilutions (six) of each extract were prepared starting from 2000 µg/mL, which corresponds with the concentration range tested in previous studies by Lorimer et al (1996), Paolini et al (2004), Houzangbe-Adote et al (2005), and Eguale et al (2006). Each experiment was examined in triplicate, with the exception of larvae third stage positive controls, which were tested in duplicate.…”

Section: Biological Proceduresmentioning

confidence: 99%

“…The larval migration inhibition (LMI) bioassay was used in order to measure inhibiting activity against infective larvae as previously described by Rabel et al (1994), Lorimer et al (1996), and Houzangbe-Adote et al (2005). Briefly, the L3 larvae hatched in vitro from T. colubriformis eggs previously obtained from infected sheep were applied at a concentration of 30-40 for each well and incubated for 3 h at 20…”

Section: Biological Proceduresmentioning

confidence: 99%

“…Among various kinds of in vitro methods, the egg hatch assays (Coles et al, 1992;Ketzis et al, 2002;Pessoa et al, 2002;Alawa et al, 2003;Hounzangbe-Adote et al, 2005) and the larval motility tests (Rabel et al, 1994;Lorimer et al, 1996;Khunkitti et al, 2000;HounzangbeAdote et al, 2005) are commonly used to test medicinal plants against nematodes.…”

Section: Introductionmentioning

confidence: 99%

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In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

Urban

Kokoška

Langrová

et al. 2008

Pharmaceutical Biology

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The ethanol extracts of 16 Czech medicinal plants, namely, . sativum, A. absinthium, C. carvi, D. carota, and J. regia possessed the strongest anthelmintic effect on the embryonating eggs at all concentrations tested (62.5, 125, 250, 500, 1000, 2000 µg/mL). The best results, showing a higher effect against the infective third-stage larvae in comparison with synthetic anthelmintic Zentel (albendazole), have been obtained for A. sativum, A. absinthium, C. carvi, C. regalis, I. helenium, J. regia, S. hortensis, and V. officinalis.

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“…Because of developing resistance to the existing anthelmintic drugs (Prichard 1990), there is a need for new anthelmintic agents. The anthelmintic activity of plant extracts can arise either from a direct action of a drug on the worms or through induction of gastrointestinal irritation and diarrhea (Lorimer et al 1996).…”

Section: Introductionmentioning

confidence: 99%

In vitro anthelmintic effect of Tobacco (Nicotiana tabacum) extract on parasitic nematode, Marshallagia marshalli

et al. 2014

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Because of developing resistance to the existing anthelmintic drugs, there is a need for new anthelmintic agents. Tobacco plant has alkaloid materials that have antiparasitic effect. We investigated the in vitro anthelminthic effect of aqueous and alcoholic extract of Tobacco (Nicotiana tabacum) against M. marshalli. For investigating this effect, we prepared three dilutions of aqueous and alcoholic extract of Tobacco (25, 50 and 75 mg/ml). The worms exposed to extracts for 10 h at 25-30°C. The buffer PBS used as negative control and 50 mg/ml dilution of Levamisole used as standard reference. In each group, 50 worms were examined. We used an inhibition mobility test for our study. Survival analysis with Cox proportional hazard model was used for data analysis. The result showed that compared with Levamisole 50 mg/ml, dilution of 25 and 50 mg/ml of the aqueous extract had the same anthelminthic effects (P [ 0.05), but 75 mg/ml dilution of the aqueous extract and dilution of 25, 50 and 75 mg/ml of alcoholic extract had more anthelminthic effect (P \ 0.05). Overall, extracts of Tobacco possess considerable anthelminthic activity and more potent effects were observed with the highest concentrations. Therefore, the in vivo study on Tobocco in animal models is recommended.

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A Nematode Larval Motility Inhibition Assay for Screening Plant Extracts and Natural Products

Cited by 61 publications

References 17 publications

Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems

Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems

In VitroAnthelmintic Effects of Medicinal Plants Used in Czech Republic

In vitro anthelmintic effect of Tobacco (Nicotiana tabacum) extract on parasitic nematode, Marshallagia marshalli

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