A near-infrared light-controlled system for reversible presentation of bioactive ligands using polypeptide-engineered functionalized gold nanorods

Yang, Jie; Yao, M.; Du, Ming-Shuo; Jin, Rui; Zhao, Donghui; Ma, Jun; Ma, Zhi-Ya; Zhao, Yuan‐Di; Liu, Bo

doi:10.1039/c4cc09516b

Cited by 20 publications

(27 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The constant immobilization of the RGD prevents unintended side effects from soluble RGD in the media and allows for multiple cycles of screening with PEG. This strategy has been further explored to incorporate a near-IR phototrigger to release the PEG molecules, thereby increasing spatiotemporal control 67 . In another example, a hydrogel was engineered to reversibly expose cell adhesion sites by folding and unfolding in response to aptamer hybridization 65 (FIG.…”

Section: Exchangeable Ligand Presentationmentioning

confidence: 99%

The design of reversible hydrogels to capture extracellular matrix dynamics

2016

View full text Add to dashboard Cite

The extracellular matrix (ECM) is a dynamic environment that constantly provides physical and chemical cues to embedded cells. Much progress has been made in engineering hydrogels that can mimic the ECM, but hydrogel properties are, in general, static. To recapitulate the dynamic nature of the ECM, many reversible chemistries have been incorporated into hydrogels to regulate cell spreading, biochemical ligand presentation and matrix mechanics. For example, emerging trends include the use of molecular photoswitches or biomolecule hybridization to control polymer chain conformation, thereby enabling the modulation of the hydrogel between two states on demand. In addition, many non-covalent, dynamic chemical bonds have found increasing use as hydrogel crosslinkers or tethers for cell signalling molecules. These reversible chemistries will provide greater temporal control of adhered cell behaviour, and they allow for more advanced in vitro models and tissue-engineering scaffolds to direct cell fate.

show abstract

Section: Exchangeable Ligand Presentationmentioning

confidence: 99%

The design of reversible hydrogels to capture extracellular matrix dynamics

2016

View full text Add to dashboard Cite

show abstract

“…Multiple photothermal agents have been successful developed, such as melanin [10], indocyanine green [11], carbon nanotubes [12] and gold nanomaterials [13]. Particularly, gold nanorods (GNR) -based nanoparticles have been extensively investigated in biomedicine due to their unique shape effects [8,14,15]. GNR can produce stable photoacoustic signals that make them trackable in vivo.…”

Section: Introductionmentioning

confidence: 99%

Macrophages loaded CpG and GNR-PEI for combination of tumor photothermal therapy and immunotherapy

et al. 2018

View full text Add to dashboard Cite

Nano-therapeutic approach for clinical implementation of tumors remains a longstanding challenge in the medical field. The main challenges are rapid clearance, offtarget effect and the limited role in the treatment of metastatic tumors. Toward this objective, a cell-mediated strategy by transporting photothermal reagents and CpG adjuvant within macrophage vehicles is performed. The photothermal reagents are constructed by conjugating of hyperbranched polyethyleimine (PEI) to golden nanorode (GNR) via S-Au bonds. GNR-PEI/CpG nanocomposites, formed via electrostatic interaction and displayed excellent near-infrared (NIR) photothermal performance, exhibit immense macrophage uptake and negligible cytotoxic effect, which is essential for the fabrication of GNR-PEI/CpG loaded macrophages. GNR-PEI/ CpG loaded macrophages demonstrated admirable photothermal response in vitro. Benefited from the functionalization of the binding adhesion between macrophages and 4T1 cells, GNR-PEI/CpG loaded macrophages significantly promoted tumor accumulation in vivo and dramatically enhanced the efficiency of photothermal cancer therapy. Moreover, the immune system is activated after photothermal therapy, which is mainly attributed to the generation of tumor specific antigens and CpG adjuvant in situ. Our findings provide a potential cell-mediated nanoplatform for tumor therapy by combination of near infrared photothermal therapy and immunotherapy.

show abstract

“…[52] Fluorescencei mages of cells adsorbed on LbL PTAPE films are shown in Figure 6a.B efore UV irradiation, the densities of adhered cellsa re 3218 AE 390 (Figure 6a1), 14909 AE 1654 (Figure 6a2), 16909 AE 2363 (Figure 6a3), and 16045 AE 927 cm À2 (Figure 6a4) on the flat and rough substrates with pillar spacingso f5 ,1 0a nd 20 mm, respectively.I ti sk nownt hat more cells can be adhered to hydrophobic surfaces than to hydrophilic surfaces. [4][5][6][7][8][9][10][11][12] The trans-azobenzene films are relatively hydrophobic, to which more cells can adhere (Figure 6a1-a4). After UV irradiation, azobenzene chromophores become cis in configuration, and have higherd ipole moments and surface free energies, and the resulting surfaces exhibit am uch lower adhesion capacity (Figure 6a5-a8).…”

Section: Resultsmentioning

confidence: 99%

“…[1][2][3] Understanding the adhesion of cells to substrates is not only helpful for preparingf unctional coatings of medical implants,b ut also significant for establishing an ext generation of interface materials, integratingc ancerc ellsi nto specific surfaces and achieving highly sensitive biosensing. In recent years, various strategies involving stimuli such as changes in pH, [4,5] temperature, [6] voltage, [7,8] and light [9][10][11][12] have been developed as triggersf or controlling cell adhesion on the surfaces. Amongt hese, the light-stimulus response system has promising potential because of its precise spatiotemporal control and convenient operation.…”

Section: Introductionmentioning

confidence: 99%

Photoswitched Cell Adhesion on Azobenzene‐Containing Self‐Assembled Films

et al. 2016

View full text Add to dashboard Cite

Stimuli-responsive surfaces that can regulate and control cell adhesion have attracted much attention for their great potential in diverse biomedical applications. Unlike for pH- and temperature-responsive surfaces, the process of photoswitching requires no additional input of chemicals or thermal energy. In this work, two different photoresponsive azobenzene films are synthesized by chemisorption and electrostatic layer-by-layer (LbL) assembly techniques. The LbL film exhibits a relatively loose packing of azobenzene chromophores compared with the chemisorbed film. The changes in trans/cis isomer ratio of the azobenzene moiety and the corresponding wettability of the LbL films are larger than those of the chemisorbed films under UV light irradiation. The tendency for cell adhesion on the LbL films decreases markedly after UV light irradiation, whereas adhesion on the chemisorbed films decreases only slightly, because the azobenzene chromophores stay densely packed. Interestingly, the tendency for cell adhesion can be considerably increased on rough substrates, the roughness being introduced by use of photolithography and inductively coupled plasma deep etching techniques. For the chemisorbed films on rough substrates, the amount of cells that adhere also changes slightly after UV light irradiation, whereas, the amount of cells that adhere to LbL films on rough substrates decreases significantly.

show abstract

A near-infrared light-controlled system for reversible presentation of bioactive ligands using polypeptide-engineered functionalized gold nanorods

Abstract: A near-infrared light-controlled hybrid platform with polypeptide-engineered functionalized gold nanorods has been designed for reversible presentation of the immobilized ligands to cell surface receptors on the engineered materials.

Cited by 20 publications

References 35 publications

The design of reversible hydrogels to capture extracellular matrix dynamics

The design of reversible hydrogels to capture extracellular matrix dynamics

Macrophages loaded CpG and GNR-PEI for combination of tumor photothermal therapy and immunotherapy

Photoswitched Cell Adhesion on Azobenzene‐Containing Self‐Assembled Films

Contact Info

Product

Resources

About