A Near Infrared Fluorescent Probe for Sensitive Determination of Human Serum Albumin

Zeng, Xiaodan; Ma, Mingshuo; Zhu, Baocun; Zhu, Lin

doi:10.2116/analsci.32.1291

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…Then, the selectivity of S1 toward HSA was assessed by testing cargo release against common interfering molecules present in biological samples. Figure 4 shows the emission of rhodamine B (at 571 nm) released from S1 nanoparticles suspended in PBS (pH 7.4)-acetonitrile 95:5 v / v in the presence of HSA and other selected interfering molecules such as anions, cations, amino acids, urea (at 10 μM) and synthetic urine in PBS [ 43 ]. As could be seen, only HSA was able to induce pore opening and rhodamine B release.…”

Section: Resultsmentioning

confidence: 99%

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

et al. 2022

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Mesoporous silica nanoparticles loaded with rhodamine B and capped with curcumin are used for the selective and sensitive fluorogenic detection of human serum albumin (HSA). The sensing mesoporous silica nanoparticles are loaded with rhodamine B, decorated with aminopropyl moieties and capped with curcumin. The nanoparticles selectively release the rhodamine B cargo in the presence of HSA. A limit of detection for HSA of 0.1 mg/mL in PBS (pH 7.4)-acetonitrile 95:5 v/v was found, and the sensing nanoparticles were used to detect HSA in spiked synthetic urine samples.

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Section: Resultsmentioning

confidence: 99%

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

et al. 2022

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“…[10][11][12][13][14][15][16][17] Recently, external fluorescent probe based detection technique has attracted much attention of the bioanalytical chemists. [18][19][20][21][22] Fluorescent based tools are costeffective, non-invasive, and rapid in nature. However, a suitable fluorophore is always required for the detection and quantification of HSA in complex biological samples.…”

Section: Introductionmentioning

confidence: 99%

Selective detection of human serum albumin by near infrared emissive fluorophores: Insights into structure-property relationship

Choudhury

Patel

Ghosh

2019

Journal of Photochemistry and Photobiology A: Chemistry

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Two donor-acceptor fluorophores were prepared and tested for quantitative determination of HSA in aqueous samples. Fluorophores were non-emissive in polar solvents due to energy loss via nonradiative decays. Complexation of the fluorophores with HSA resulted multi-fold enhancement of emission in the red-near infrared (NIR) region. The emission intensity was linearly correlated to the amount of protein in the solution, which enabled us to develop calibration graphs for quantitative estimation of HSA in synthetic urine samples. Between the two fluorophores, the methoxy substituted fluorophore 1 selectively recognized HSA. It exhibited remarkable fluorescence enhancement with HSA over bovine serum albumin (BSA) and other globular proteins. The selective sensing aptitude of 1 was attributed to its restricted motions in the protein's microenvironment due to multiple non-covalent interactions, preventing energy loss by radiationless decay. The different recognition properties of the fluorophores were estimated by the steady-state fluorescence and molecular docking studies. These findings indicate that this class of fluorophores can be useful for quantitative estimation of HSA in biological urine and blood samples in clinical practice.

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“…For example, research has shown that there is an intimate connection between cardiovascular disease and kidney damage, and the critical indictor for kidney damage is the concentration of HSA in human urine [ 5 ]. Several methods to determine the concentration of HSA have been developed, including visible absorption spectroscopy [ 6 , 7 , 8 ], electrochemical [ 9 , 10 ], Immunoassays [ 11 , 12 , 13 ] and High-performance liquid chromatography (HPLC) [ 14 ]; however, there are some problems for detecting the concentration of HSA with these methods. For example, Tu and co-workers reported a low-cost method with Immunoassays to detect the HSA [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

Chen

Xiang

et al. 2017

Sensors

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In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610 nm. However, in the presence of HSA, it will absorb cupric ion, which will prevent the formation of CuNPs. As a result, the fluorescence intensity will become obviously lower in the presence of HSA. The analyte HSA concentration had a proportional linear relationship with the fluorescence intensity of CuNPs. The detection limit for HSA was 8.2 × 10−8 mol·L−1. Furthermore, it was also successfully employed to determine HSA in biological samples. Thus, this method has potential applications in point-of-care medical diagnosis and biomedical research.

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A Near Infrared Fluorescent Probe for Sensitive Determination of Human Serum Albumin

Cited by 7 publications

References 20 publications

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Fluorogenic Detection of Human Serum Albumin Using Curcumin-Capped Mesoporous Silica Nanoparticles

Selective detection of human serum albumin by near infrared emissive fluorophores: Insights into structure-property relationship

A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

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