A natural rubber drainage tube with antithrombogenic lumen surface

Klöcking, H.-P.; Schunk, W; Merkmann, G.; Gieβmann, C.; Knoll, H.; Borgmann, S. H. M.

doi:10.1016/0049-3848(93)90110-a

Cited by 5 publications

(6 citation statements)

References 10 publications

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“…TEG analysis performed on the perfused plasma showed inhibition of clotting due to the release of pentosan polysulphate sodium into the plasma. 28 Chevallier et al 29 used a similar method to measure the coagulability of blood on phosphorylcholine grafted expanded polytetrafluoroethylene (ePTFE) tubes. In this study, they added blood to the surface of the phosphorylcholine grafted ePTFE tubes and incubated for 30 min and 37 C without shear.…”

Section: Introductionmentioning

confidence: 99%

Standardized methods to quantify thrombogenicity of blood‐contacting materials via thromboelastography

et al. 2011

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Blood coagulation is the most significant complication of vascular biomaterials. A straightforward, sensitive, and standard measure of the compatibility of these materials with whole blood (hemocompatibility) is necessary to avoid coagulation. Current techniques used quantify only individual clotting components and are poor predictors of coagulation. The thromboelastograph (TEG) provides a measure of overall clot formation from whole blood. Although TEG is very common in clinical settings, its application to biomaterials is limited partly due to difficulty in sample preparation. In this protocol, whole blood samples are incubated with (1) biomaterials (tube with clamped ends) and (2) endothelial cells cultured on biomaterial surfaces (12-well plate) under controlled shearing conditions (10 rpm on rocker, at 37°C), and then the blood is transferred to the TEG to measure clot formation. TEG clearly discriminates among the R-times (time until initial clot formation) of expanded poly(tetrafluoroethylene), poly(urethane), and Tygon tubing. Marked differences in R-time are also seen when endothelial cells are cultured on various extracellular matrix proteins and proteoglycans. Thus, R-time provides a robust metric of overall thrombogenicity of biomaterials, and these procedures provide a standardized method for TEG to facilitate direct comparison among candidate biomaterials undergoing in-vitro testing.

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Section: Introductionmentioning

confidence: 99%

Standardized methods to quantify thrombogenicity of blood‐contacting materials via thromboelastography

et al. 2011

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“…It shows a variety of biomaterials: both polymer and ceramic biomaterials have been studied using a variety of TEG methods. The polymer biomaterials include biopolymers in resuscitation fluids, [26][27][28][29][30][31][32][33][34][35] other blood-soluble polymers and peptide derivatives dissolved in saline or aqueous buffers, [36][37][38][39][40][41][42][43][44][45] hydrogels, 46 polymers in the forms of surface coatings 47 and additives, 48 polymer nanoparticles, 49,50 fibers, 51 films, [52][53][54] and in situ forming hydrogels. [55][56][57] The ceramic biomaterials include zeolites, 58 bioactive glasses, 59,60 metal oxides, 61 and an amorphous alloy of silicon and carbon.…”

Section: Methodsmentioning

confidence: 99%

“…Partial blood including platelet‐rich, platelet‐poor, and coagulation factor‐deficient plasma was used to understand a specific coagulation pathway 70, 71. There are a few studies where partial coagulation systems were used to understand the interactions of a biomaterial with a specific blood component 37, 45, 48, 51, 53, 62. In addition to blood, different activators such as celite, kaolin, and tissue factor were used to reduce the measurement time 72.…”

Section: Methodsmentioning

confidence: 99%

“…70,71 There are a few studies where partial coagulation systems were used to understand the interactions of a biomaterial with a specific blood component. 37,45,48,51,53,62 In addition to blood, different activators such as celite, kaolin, and tissue factor were used to reduce the measurement time. 72 Quality controls for the analyzer need to be done on a regular basis, which consists of maintenance and function checks (e.g., electronic testing), calibration, and calibration verification using manufacturer's two-level biological controls.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to coating materials, TEG can evaluate the antithrombogenic effects of polymer additives in a base biomaterial 48. Pentosan polysulphate sodium bound to a zeolite was incorporated into a natural rubber drainage tube during manufacturing process.…”

Section: Polymer Biomaterialsmentioning

confidence: 99%

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Thromboelastographic study of biomaterials

Peng

2010

J Biomed Mater Res

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Thromboelastography (TEG) is drawing more attention for clinical and in vitro studies of blood coagulation. It can be applied to evaluate the effects of both blood-soluble and insoluble biomaterials on whole blood coagulation from the beginning of coagulation through clot formation to the ending with fibrinolysis. TEG may also identify the relative contributions of various clotting factors, such as fibrinogen and platelets, to the overall coagulation process based on profiles of its variables using whole and partial blood components. A comprehensive review has been conducted on its applications for the assessment of a wide range of blood-contacting biomaterials ranging from polymers to ceramics and biomedical devices involved in many applications. The methodology is different in terms of instrumentation, the methods to activate blood coagulation, the type of blood (citrated versus fresh blood), and study settings (in vitro, in vivo, and clinical trials). The author's own work and future directions are discussed as well. TEG should be considered as one of the most useful tools for evaluating in vitro and in vivo blood-biomaterial interactions for different applications.

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Modification of a commercial thromboelastography instrument to measure coagulation dynamics with three-dimensional biomaterials

2016

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Three-dimensional synthetic constructs with complex geometries have immense potential for use in a multitude of blood-contacting applications. Understanding coagulation phenomena is arguably the most critical aspect for applications involving synthetic biomaterials; however, real-time evaluation of the clot formation while interfacing with these materials is difficult to achieve in a reproducible and robust manner. Here, work representing first steps toward addressing this deficit is presented, wherein modified consumables for a clinical instrument (a Thromboelastograph(®)) have been fabricated. Thromboelastography (TEG) measures viscoelastic properties throughout clot formation and therefore provides clinically relevant coagulation measurements in real time (i.e., kinetics and strength of clot formation). Through our modification, TEG consumables can readily accommodate three-dimensional materials (e.g., those for regenerative tissue applications). The authors performed proof-of-concept experiments using polymer scaffolds with a range of surface properties and demonstrated that variations in surface properties resulted in differences in blood plasma coagulation dynamics. For example, the maximum rate of thrombus generation ranged from 22.2 ± 2.2 (dyn/cm(2))/s for fluorocarbon coated scaffolds to 8.7 ± 1.0 (dyn/cm(2))/s for nitrogen-containing scaffolds. Through this work, the ability to make real-time coagulation activity measurements during constant coagulation factor interface with biomedically relevant materials is demonstrated.

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A natural rubber drainage tube with antithrombogenic lumen surface

Cited by 5 publications

References 10 publications

Standardized methods to quantify thrombogenicity of blood‐contacting materials via thromboelastography

Standardized methods to quantify thrombogenicity of blood‐contacting materials via thromboelastography

Thromboelastographic study of biomaterials

Modification of a commercial thromboelastography instrument to measure coagulation dynamics with three-dimensional biomaterials

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