A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 ( CYP1 )

Gaisne, Mauricette; Bécam, A.‐M.; Verdière, Jacqueline; Herbert, Christopher J.

doi:10.1007/s002940050490

Cited by 135 publications

(127 citation statements)

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“…The trans eQTLs for all six genes map to the same marker on chromosome 12, and the likely causal polymorphism underlying this eQTL is already known, a Ty1 retrotransposon insertion in the activation domain of HAP1, a transcription factor known to regulate the ERG pathway. This insertion has been shown to substantially reduce the activity of HAP1 and to alter regulation of the HMG1 and CYC1 promoters (20,21). Surprisingly, the insertion is quite recent: it is not present in either W303 or CEN.PK (20) (or any other of more than 70 partially sequenced yeast strains) (18), suggesting that it occurred in the BY-specific branch (Fig.…”

Section: Resultsmentioning

confidence: 97%

Evidence for widespread adaptive evolution of gene expression in budding yeast

Fraser

Moses

Schadt

2010

Proc. Natl. Acad. Sci. U.S.A.

155

183

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Changes in gene expression have been proposed to underlie many, or even most, adaptive differences between species. Despite the increasing acceptance of this view, only a handful of cases of adaptive gene expression evolution have been demonstrated. To address this discrepancy, we introduce a simple test for lineage-specific selection on gene expression. Applying the test to genome-wide gene expression data from the budding yeast Saccharomyces cerevisiae , we find that hundreds of gene expression levels have been subject to lineage-specific selection. Comparing these findings with independent population genetic evidence of selective sweeps suggests that this lineage-specific selection has resulted in recent sweeps at over a hundred genes, most of which led to increased transcript levels. Examination of the implicated genes revealed a specific biochemical pathway—ergosterol biosynthesis—where the expression of multiple genes has been subject to selection for reduced levels. In sum, these results suggest that adaptive evolution of gene expression is common in yeast, that regulatory adaptation can occur at the level of entire pathways, and that similar genome-wide scans may be possible in other species, including humans.

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Section: Resultsmentioning

confidence: 97%

Evidence for widespread adaptive evolution of gene expression in budding yeast

Fraser

Moses

Schadt

2010

Proc. Natl. Acad. Sci. U.S.A.

155

183

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“…Strains derived from S288c (such as BY4742) have mutant forms of several genes including a mutant allele of HAP1, which encodes a heme-responsive transcriptional regulator (Gaisne et al 1999). This mutation, caused by an insertion near the 39 end of the HAP1 ORF, has a detrimental effect on some, but not all, Hap1p targets (Gaisne et al 1999).…”

Section: Resultsmentioning

confidence: 99%

A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae

Davies¹,

2006

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Upc2p and Ecm22p are a pair of transcription factors responsible for the basal and induced expression of genes encoding enzymes of ergosterol biosynthesis in yeast (ERG genes). Upc2p plays a second role as a regulator of hypoxically expressed genes. Both sterols and heme depend upon molecular oxygen for their synthesis, and thus the levels of both have the potential to act as indicators of the oxygen environment of cells. Hap1p is a heme-dependent transcription factor that both Upc2 and Ecm22p depend upon for basal level expression of ERG genes. However, induction of both ERG genes and the hypoxically expressed DAN/ TIR genes by Upc2p and Ecm22p occurred in response to sterol depletion rather than to heme depletion. Indeed, upon sterol depletion, Upc2p no longer required Hap1p to activate ERG genes. Mot3p, a broadly acting repressor/activator protein, was previously shown to repress ERG gene expression, but the mechanism was unclear. We established that Mot3p bound directly to Ecm22p and repressed Ecm22p-but not Upc2p-mediated gene induction.

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“…The other yeast strain used in this study, BY, derived from the S288c strain, which bears a mutation in the HAP1 gene (51). HAP1 encodes a transcription factor involved, on the one hand, in the expression of aerobic genes in response to [O 2 ] increase, and, on the other hand, in the activation of ROX1, a gene involved in the downregulation of anaerobic genes under normoxic conditions.…”

Section: Discussionmentioning

confidence: 99%

Questioning the functional relevance of mitochondrial supercomplexes by time-resolved analysis of the respiratory chain

Trouillard

Meunier

Rappaport

2011

Proc. Natl. Acad. Sci. U.S.A.

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Mitochondria are the powerhouses of eukaryotic cells as they feed metabolism with its major substrate. Oxidative-phosphorylation relies on the generation, by an electron/proton transfer chain, of an electrochemical transmembrane potential utilized to synthesize ATP. Although these fundamental principles are not a matter of debate, the emerging picture of the respiratory chain diverges from the linear and fluid scheme. Indeed, a growing number of pieces of evidence point to membrane compartments that possibly restrict the diffusion of electron carriers, and to supramolecular assembly of various complexes within various kinds of supercomplexes that modulate the thermodynamic and kinetic properties of the components of the chain. Here, we describe a method that allows the unprecedented time-resolved study of the respiratory chain in intact cells that is aimed at assessing these hypotheses. We show that, in yeast, cytochrome c is not trapped within supercomplexes and encounters no particular restriction to its diffusion which questions the functional relevance of these supramolecular edifices.bioenergetics | electon transfer | respiration | compartmentalization

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A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 ( CYP1 )

Cited by 135 publications

References 0 publications

Evidence for widespread adaptive evolution of gene expression in budding yeast

Evidence for widespread adaptive evolution of gene expression in budding yeast

A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae

Questioning the functional relevance of mitochondrial supercomplexes by time-resolved analysis of the respiratory chain

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