A natural gene drive system confers reproductive isolation in rice

Wang, Chaolong; Wang, Jian; Lu, Jiayu; Xiong, Ye; Zhao, Zhigang; Yu, Xiaowen; Zheng, Xiaoming; Li, Jing; Lin, Qian; Ren, Yulong; Yang, Hu; He, Xiaodong; Li, Chao; Zeng, Yonglun; Miao, Ran; Guo, Mali; Zhang, Bosen; Zhu, Ying; Zhang, Yunhui; Tang, Weijie; Wang, Yunlong; Hao, Benyuan; Wang, Qiming; Cheng, Siqi; He, Xiaohua; Yao, Bowen; Gao, Junwen; Zhu, Xufei; Yu, Hao; Wang, Yong; Sun, Yi; Zhou, Chunlei; Dong, Hui; Ma, Xiaoding; Guo, Xiuping; Liu, Xi; Tian, Yunlu; Liu, Shijia; Wang, Chunming; Cheng, Zhijun; Jiang, Ling; Zhou, Jiawu; Guo, Hui‐Shan; Jiang, Liwen; Tao, Dayun; Chai, Jijie; Zhang, Wei; Wang, Haiyang; Wu, Chuanyin; Wan, Jianmin

doi:10.1016/j.cell.2023.06.023

Cited by 21 publications

(5 citation statements)

References 60 publications

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“…However, efforts to introduce traits that benefit humans are almost invariably limited by classical Mendelian inheritance and Darwinian selection because they are selectively neutral or even detrimental to the organisms themselves and are therefore rapidly lost in targeted populations. Nevertheless, selfish genetic elements are prevalent in nature and can be transmitted to progeny at super-Mendelian (>50%) frequencies [1][2][3] . Inspired by these natural processes, synthetic gene drives have been proposed for spreading genetic alterations throughout populations in their natural environment, despite potential fitness costs [4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Overriding Mendelian inheritance inArabidopsiswith a CRISPR toxin-antidote gene drive that impairs pollen germination

Liu,

Jiao,

Champer

et al. 2023

Preprint

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Synthetic gene drives, inspired by natural selfish genetic elements, present transformative potential for disseminating traits that benefit humans throughout wild populations, irrespective of potential fitness costs. Here, we constructed a gene drive system called CRISPR-Assisted Inheritance utilizing NPG1 (CAIN), which employs a toxin-antidote mechanism in the male germline to override Mendelian inheritance in plants. Specifically, a gRNA-Cas9 cassette targets the essential No Pollen Germination 1 (NPG1) gene, serving as the toxin to block pollen germination. A recoded, CRISPR-resistant copy of NPG1 serves as the antidote, providing rescue only in pollen cells that carry the drive. To limit potential consequences of inadvertent release, we used self-pollinating Arabidopsis thaliana as a model. The drive demonstrated a robust 88-99% transmission rate over two successive generations, producing minimal resistance alleles that are unlikely to inhibit drive spread. Our study provides a strong basis for rapid genetic modification or suppression of outcrossing plant populations.

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Section: Introductionmentioning

confidence: 99%

Overriding Mendelian inheritance inArabidopsiswith a CRISPR toxin-antidote gene drive that impairs pollen germination

Liu,

Jiao,

Champer

et al. 2023

Preprint

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“…CRISPR/Cas9 editing ZmRAVL1, a positive regulator of leaf angle, engineered an upright plant architecture with increased yield under high planting densities, providing an option to develop density-tolerant high-yield cultivars [21,22]. Using CRISPR/Cas technology, the DUYAO was identified as the candidate gene of RHS12 locus controlling male gamete sterility in indica-japonica inter-subspecific hybrid rice, which clarified the genetic basis of reproductive isolation and provided a strategy for molecular designs of hybrid rice breeding [23].…”

Section: Exploring Gene Functions and Creating Desired Germplasmsmentioning

confidence: 99%

CRISPR/Cas Technology Revolutionizes Crop Breeding

Tang,

Wang,

Jin

et al. 2023

Plants

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Crop breeding is an important global strategy to meet sustainable food demand. CRISPR/Cas is a most promising gene-editing technology for rapid and precise generation of novel germplasm and promoting the development of a series of new breeding techniques, which will certainly lead to the transformation of agricultural innovation. In this review, we summarize recent advances of CRISPR/Cas technology in gene function analyses and the generation of new germplasms with increased yield, improved product quality, and enhanced resistance to biotic and abiotic stress. We highlight their applications and breakthroughs in agriculture, including crop de novo domestication, decoupling the gene pleiotropy tradeoff, crop hybrid seed conventional production, hybrid rice asexual reproduction, and double haploid breeding; the continuous development and application of these technologies will undoubtedly usher in a new era for crop breeding. Moreover, the challenges and development of CRISPR/Cas technology in crops are also discussed.

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“…A Y-drive system in Anopheles gambiae destroyed X-chromosomes during spermatogenesis and produced 95% male progeny [62]. The selective killers that work on the toxin-antidote system were identified in rice loci qHMS7 and OsCOX11, where genes ORF2 and ORF3 for locus qHMS7, and DUYAO and JIEYAO for locus OsCOX11, encoded for toxins and antidotes, respectively [63,64].…”

Section: Meiotic Drivementioning

confidence: 99%

Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9

Kumam,

Trick,

Vara Prasad

et al. 2023

Genes

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Weeds can negatively impact crop yields and the ecosystem’s health. While many weed management strategies have been developed and deployed, there is a greater need for the development of sustainable methods for employing integrated weed management. Gene drive systems can be used as one of the approaches to suppress the aggressive growth and reproductive behavior of weeds, although their efficacy is yet to be tested. Their popularity in insect pest management has increased, however, with the advent of CRISPR-Cas9 technology, which provides specificity and precision in editing the target gene. This review focuses on the different types of gene drive systems, including the use of CRISPR-Cas9-based systems and their success stories in pest management, while also exploring their possible applications in weed species. Factors that govern the success of a gene drive system in weeds, including the mode of reproduction, the availability of weed genome databases, and well-established transformation protocols are also discussed. Importantly, the risks associated with the release of weed populations with gene drive-bearing alleles into wild populations are also examined, along with the importance of addressing ecological consequences and ethical concerns.

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A natural gene drive system confers reproductive isolation in rice

Cited by 21 publications

References 60 publications

Overriding Mendelian inheritance inArabidopsiswith a CRISPR toxin-antidote gene drive that impairs pollen germination

Overriding Mendelian inheritance inArabidopsiswith a CRISPR toxin-antidote gene drive that impairs pollen germination

CRISPR/Cas Technology Revolutionizes Crop Breeding

Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9

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