A nascent protein labeling strategy disclosed mitochondrial proteomic responses in punicalagin intervened insulin resistance of HepG2 cells

Zhang, Zhengyi; Zeng, Mengqi; Han, Xiao; Hou, Zhanwu; Wang, Zhen; Su, Tian; Zhao, Wei; Liu, Huadong; Liu, Jiankang

doi:10.1039/d1fo02749b

Cited by 6 publications

(3 citation statements)

References 38 publications

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“…The labeling method of the nascent proteins is in agreement with the previous report of our laboratory ( 38 ). In briefly, HepG2 cells were treated with or without PA (300 μM) for 24 h, then label with AHA.…”

Section: Methodssupporting

confidence: 89%

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4EBP2-regulated protein translation has a critical role in high-fat diet–induced insulin resistance in hepatocytes

Han,

Yang,

Zhang

et al. 2023

Journal of Biological Chemistry

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Section: Methodssupporting

confidence: 89%

“…We aimed to investigate the effects of PA-stimulated insulin resistance on nascent proteomics changes using an AHA labeling technique commonly used to probe changes in protein translation ( 37 , 38 ). Phosphorylation proteome analysis indicated that protein translation regulation was involved in PA treatment.…”

Section: Resultsmentioning

confidence: 99%

4EBP2-regulated protein translation has a critical role in high-fat diet–induced insulin resistance in hepatocytes

Han,

Yang,

Zhang

et al. 2023

Journal of Biological Chemistry

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“…After treatment, the cells were washed with 1× PBS; then, JC-1 staining was analyzed via fluorescence microscopy or scanned with a microplate fluorometer (Fluoroskan Ascent, Thermo Fisher Scientific Inc., Waltham, MA, USA) at 488 nm excitation and with 535 and 590 nm emissions to measure the green and red JC-1 fluorescence, respectively. Each well was scanned by measuring the intensity of each of the 25 squares (of 1 mm 2 area) arranged in a 5 × 5 rectangular array [33].…”

Section: Jc-1 Assay For Determining Mitochondrial Membrane Potential ...mentioning

confidence: 99%

The Role of Nicotinamide Mononucleotide Supplementation in Psoriasis Treatment

Zhang,

Cheng,

et al. 2024

Antioxidants

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Psoriasis is one of several chronic inflammatory skin diseases with a high rate of recurrence, and its pathogenesis remains unclear. Nicotinamide mononucleotide (NMN), as an important precursor of nicotinamide adenine dinucleotide (NAD+), has been reported to be a promising agent in treating various diseases, its positive effects including those induced via its anti-inflammatory and antioxidant properties. For this reason, we have aimed to explore the possible role of NMN in the treatment of psoriasis. Psoriasis models were constructed with imiquimod (IMQ) stimulation for 5 days in vivo and with M5 treatment in keratinocyte cell lines in vitro. NMN treatment during the IMQ application period markedly attenuated excess epidermal proliferation, splenomegaly, and inflammatory responses. According to GEO databases, Sirtuin1 (SIRT1) levels significantly decreased in psoriasis patients’ lesion tissues; this was also the case in the IMQ-treated mice, while NMN treatment reversed the SIRT1 decline in the mouse model. Moreover, NMN supplementation also improved the prognoses of the mice after IMQ stimulation, compared to the untreated group with elevated SIRT1 levels. In HEKa and HaCaT cells, the co-culturing of NMN and M5 significantly decreased the expression levels of proinflammation factors, the phosphorylation of NF-κB, stimulator of interferon genes (STING) levels, and reactive oxygen species levels. NMN treatment also recovered the decrease in mitochondrial membrane potential and respiration ability and reduced mtDNA in the cytoplasm, leading to the inhibition of autoimmune inflammation. The knockdown of SIRT1 in vitro eliminated the protective and therapeutic effects of NMN against M5. To conclude, our results indicate that NMN protects against IMQ-induced psoriatic inflammation, oxidative stress, and mitochondrial dysfunction by activating the SIRT1 pathway.

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New insights into the role of mitochondrial dynamics in oxidative stress-induced diseases

Chen,

Li,

Shi

et al. 2024

Biomedicine & Pharmacotherapy

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A nascent protein labeling strategy disclosed mitochondrial proteomic responses in punicalagin intervened insulin resistance of HepG2 cells

Abstract: Insulin Resistance (IR), as the common pathophysiological basis, is closely related to a variety of metabolic diseases, such as obesity and diabetes. IR is often accompanied with mitochondrial dysfunction which...

Cited by 6 publications

References 38 publications

4EBP2-regulated protein translation has a critical role in high-fat diet–induced insulin resistance in hepatocytes

4EBP2-regulated protein translation has a critical role in high-fat diet–induced insulin resistance in hepatocytes

The Role of Nicotinamide Mononucleotide Supplementation in Psoriasis Treatment

New insights into the role of mitochondrial dynamics in oxidative stress-induced diseases

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