A nanotube based electron microbeam cellular irradiator for radiobiology research

Bordelon, David E.; Zhang, Jian; Graboski, S; Cox, Adrienne D.; Schreiber, Eric; Zhou, Otto; Chang, S

doi:10.1063/1.3043417

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…In this work, a cascade of three distinct events associated with DNA damage that are known to be linearly related to absorbed dose were examined. A similar biological characterization of a microbeam cellular irradiator using γH2AX dephosphorylation has previously been presented by Bordelon et al [ 13 ]. However, changes in γH2AX can be dependent on multiple cellular factors.…”

Section: Discussionmentioning

confidence: 68%

“…While several novel irradiation devices have been developed in the last decade these mainly focus on producing thin micro-beams that measure several hundred microns in width and require custom fabricated cellular growth plates that are incompatible with standard bioassay instrumentation [ 13 , 14 ]. Our system was designed from the beginning with the radiobiology researcher in mind to deliver highly uniform, full-well dose to standard cell culture plates.…”

Section: Discussionmentioning

confidence: 99%

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Biological characterization of a novel in vitro cell irradiator

et al. 2017

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To evaluate the overall robustness of a novel cellular irradiator we performed a series of well-characterized, dose-responsive assays to assess the consequences of DNA damage. We used a previously described novel irradiation system and a traditional 137Cs source to irradiate a cell line. The generation of reactive oxygen species was assessed using chloromethyl-H2DCFDA dye, the induction of DNA DSBs was observed using the comet assay, and the initiation of DNA break repair was assessed through γH2AX image cytometry. A high correlation between physical absorbed dose and biologic dose was seen for the production of intracellular reactive oxygen species, physical DNA double strand breaks, and modulation of the cellular double stand break pathway. The results compared favorably to irradiation with a traditional 137Cs source. The rapid, straightforward tests described form a reasonable approach for biologic characterization of novel irradiators. These additional testing metrics go beyond standard physics testing such as Monte Carlo simulation and thermo-luminescent dosimeter evaluation to confirm that a novel irradiator can produce the desired dose effects in vitro. Further, assessment of these biological metrics confirms that the physical handling of the cells during the irradiation process results in biologic effects that scale appropriately with dose.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Biological characterization of a novel in vitro cell irradiator

et al. 2017

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“…The nanotube-based electron microbeam irradiator was introduced in 2008. 94 This was followed by Monte Carlo calculations for a compact nanotube microbeam system. 95 The first biological paper showing the effects of such beamlets was for brain tissue.…”

Section: Grid-based Radiation Therapymentioning

confidence: 99%

Microbeam radiation therapy — grid therapy and beyond: a clinical perspective

Schültke

Balosso

Breslin

et al. 2017

The British Journal of Radiology

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Microbeam irradiation is spatially fractionated radiation on a micrometer scale. Microbeam irradiation with therapeutic intent has become known as microbeam radiation therapy (MRT). The basic concept of MRT was developed in the 1980s, but it has not yet been tested in any human clinical trial, even though there is now a large number of animal studies demonstrating its marked therapeutic potential with an exceptional normal tissue sparing effect. Furthermore, MRT is conceptually similar to macroscopic grid based radiation therapy which has been used in clinical practice for decades. In this review, the potential clinical applications of MRT are analysed for both malignant and non-malignant diseases.

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“…Each of the cathode segment/pixel can be individually programmable within the same x-ray tube. [21][22][23][24] Our multidisciplinary research team has pioneered the development of a number of novel imaging and irradiation devices based on CNT field emission technology, including micro-CT, 25,26 multipixel stationary breast tomosynthesis, 27 multipixel stationary tomosynthesis for image-guided radiotherapy, 28 micro-CT-RT, 22 and image-guided single cell irradiation. 23,29 Based on the same nanotechnology, we propose a novel compact MRT irradiation system.…”

Section: Ib Carbon Nanotube Field Emission Technologymentioning

confidence: 99%