A nanolitre method to determine the hydrodynamic radius of proteins and small molecules by Taylor dispersion analysis

Hulse, Wendy L.; Forbes, Robert T.

doi:10.1016/j.ijpharm.2011.03.040

Cited by 37 publications

(26 citation statements)

References 30 publications

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“…proteincrowded suspensions, high particle concentration etc.) or samples where only limited sample preparation is possible [9][10][11]. There are possibilities to overcome some of these problems, for example by the use of depolarized dynamic light scattering, as described by Balog et al [12].…”

Section: Introductionmentioning

confidence: 99%

“…In other words, larger NPs will be dispersed more strongly by convection than smaller ones, and by detecting the band broadening of these concentration fronts, and obtaining so-called Taylorgrams of the NPs, one can determine hydrodynamic sizes by fitting the Taylorgram against a Gaussian function [13][14][15]. In the past, TDA has mainly been used to observe chemical reactions [16,17], or to determine the diffusion coefficient of small molecules, proteins, and polymers [10,11,[18][19][20]. With the increasing interest in nanomaterials, some work has been done in the past [9,13,15,18,[21][22][23][24] to investigate whether TDA can be used for NP characterization.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown several advantages of this method, e.g. short measurement times [9,11,17], small sample volumes (i.e. nanoliters) [11,25].…”

Section: Introductionmentioning

confidence: 99%

“…short measurement times [9,11,17], small sample volumes (i.e. nanoliters) [11,25]. Furthermore, the method is not only limited to spherical NPs but can be applied to other shapes, such as rods [18].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the method is not only limited to spherical NPs but can be applied to other shapes, such as rods [18]. However, research focused on NP characterization with TDA has been focused on polymeric samples with a limited number of samples and few different sizes [9][10][11]18,25]. Due to the scope of this manuscript we opted to compile a list of additional literature concerning Taylor Dispersion and nanoparticle analysis and add it to the supplementary information rather than continue here.…”

Section: Introductionmentioning

confidence: 99%

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Taylor Dispersion of Inorganic Nanoparticles and Comparison to Dynamic Light Scattering and Transmission Electron Microscopy

Urban

Milošević

Bossert

et al. 2018

Colloid and Interface Science Communications

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A B S T R A C TTaylor dispersion analysis (TDA) is an analytical method that has so far mainly been utilized to determine the diffusion coefficient of small molecules, and proteins. Due to increasing interest in nanoscience, some research has been done on the applicability of TDA towards characterizing nanoparticles. This work aims to expand this knowledge and give insight into the range for which TDA can be used for nanoparticle characterization, focusing on various materials and sizes. The TDA setup shown in this work was successful in characterizing all engineered metallic, non-metallic nanoparticles, and proteins tested in this work. Results were compared to dynamic light scattering and electron microscopy, and were in good agreement with both methods. Taking into consideration the wide range of nanoparticle sizes that can be characterized, the minimal sample preparation, and sample volume, required and the simplicity of the method, TDA can be considered as a valuable technique for nanoparticle characterization.When looking at the huge array of properties that nanoparticles (NPs) possess, it is not surprising that they have found their way into a multitude of scientific research areas, and industrial applications [1]. Interesting NP phenomena are mostly governed by their size, and for many applications it is imperative that the NPs not only have a very specific size but also display a narrow particle size distribution [2]. For example, the heating properties of superparamagnetic iron oxide nanoparticles (SPIONs), which are being investigated as mediators of hyperthermia in cancer treatment, are dependent on their size and size distribution. Therefore, the characterization of nanoparticle size is crucial to ensure their functionality [3]. For this purpose, several analytical methods such as dynamic light scattering (DLS), NP tracking analysis (NTA), UV-Vis spectroscopy, field-flow fractionation, analytical ultracentrifugation, and transmission electron microscopy (TEM) have been utilized to characterize NP sizes and size distributions [4][5][6][7][8]. Each method has its advantages and disadvantages, and a combination of techniques is typically recommended to adequately characterize NPs [4]. For example, TEM provides information about NP core sizes, but cannot evaluate NP hydrodynamic diameters. Conversely, DLS and NTA can evaluate particle hydrodynamic diameter and colloidal stability, but are limited by the quality of light scattering and require a deeper knowledge of the theory and model-fitting to properly analyze the raw data. With scattering-based techniques, the limit of detection for NPs depends on the sensitivity of the detection of scattered light, and factors such as the material refractive index, particle size, shape and the wavelength used for detection. Furthermore, standard DLS measurements struggle with analyzing NPs in complex environments (e.g. proteincrowded suspensions, high particle concentration etc.) or samples where only limited sample preparation is possible [9][10][11]. There are pos...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown several advantages of this method, e.g. short measurement times [9,11,17], small sample volumes (i.e. nanoliters) [11,25].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Taylor Dispersion of Inorganic Nanoparticles and Comparison to Dynamic Light Scattering and Transmission Electron Microscopy

Urban

Milošević

Bossert

et al. 2018

Colloid and Interface Science Communications

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Development of a P((MAA‐co‐NVP)‐g‐EG) Hydrogel Platform for Oral Protein Delivery: Effects of Hydrogel Composition on Environmental Response and Protein Partitioning

Steichen

O'Connor

Peppas

2016

Macromolecular Bioscience

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Hydrogels based upon terpolymers of methacrylic acid, N-vinyl pyrrolidone, and poly(ethylene glycol) are developed and characterized for their ability to respond to changes in environmental pH and to partition protein therapeutics of varying molecular weights and isoelectric points. P((MAA-co-NVP)-g-EG) hydrogels are synthesized with PEG-based cross-linking agents of varying length and incorporation densities. The composition is confirmed using FT-IR spectroscopy and shows peak shifts indicating hydrogen bonding. Scanning electron microscopy reveals microparticles with an irregular, planar morphology. The pH-responsive behavior of the hydrogels is confirmed under equilibrium and dynamic conditions, with the hydrogel collapsed at acidic pH and swollen at neutral pH. The ability of the hydrogels to partition model protein therapeutics at varying pH and ionic strength is evaluated using three model proteins: insulin, porcine growth hormone, and ovalbumin. Finally, the microparticles are evaluated for adverse interactions with two model intestinal cell lines and show minimal cytotoxicity at concentrations below 5 mg mL .

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Particulate Matter in Sterile Parenteral Products

Singh

2013

Sterile Product Development

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A nanolitre method to determine the hydrodynamic radius of proteins and small molecules by Taylor dispersion analysis

Cited by 37 publications

References 30 publications

Taylor Dispersion of Inorganic Nanoparticles and Comparison to Dynamic Light Scattering and Transmission Electron Microscopy

Taylor Dispersion of Inorganic Nanoparticles and Comparison to Dynamic Light Scattering and Transmission Electron Microscopy

Development of a P((MAA‐co‐NVP)‐g‐EG) Hydrogel Platform for Oral Protein Delivery: Effects of Hydrogel Composition on Environmental Response and Protein Partitioning

Particulate Matter in Sterile Parenteral Products

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