Lubrication failure is one of the main failure forms of gear failure. Time varying meshing stiffness is an important factor affecting the dynamic behavior of gears. However, the influence of oil film stiffness is usually ignored in the research process. In this paper, according to the meshing characteristics of double involute gears, based on the non-Newtonian thermal EHL theory, a new calculation method of normal and tangential oil film stiffness for double involute gears is established by the idea of subsection method. The oil film stiffness difference between double involute gears and common involute gears is analyzed, and the influence of tooth waist order parameters, working conditions, and thermal effect on the oil film stiffness are studied. The results reveal that there are some differences between normal and tangential oil film stiffness between double involute gears and common involute gears, but there is little difference. Compared with the torque, rotation speed and initial viscosity of the lubricating oil, the tooth waist order parameters have less influence on the oil film stiffness. Thermal effect has a certain influence on normal and tangential oil film stiffness, which indicates that the influence of thermal effect on the oil film can not be ignored. This research proposes a calculation method of normal and tangential oil film stiffness suitable for double involute gears, which provides a theoretical basis for improving the stability of the transmission.