A nano-fibrous assembly of collagen–hyaluronic acid for controlling cell-adhesive properties

Fujie, Toshinori; Furutate, Sho; Niwa, Daisuke; Takeoka, Shinji

doi:10.1039/c0sm00527d

Cited by 29 publications

(34 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fujie et al [99] synthesized ultrathin films, composed of PLLA and showing different stiffnesses, obtained by changing their thickness; H9c2 cells (an embryonic myocardium rat cell line) were then cultured onto these substrates. The adhesion properties of H9c2 on these nanofilms were valued by estimating adhesion area and elongation ratio of cells, highlighting similar results to previously reported ones [97]: cells preferentially adhered on rigid substrates in comparison to soft ones. Pensabene et al [100] used single similar nanosheets to sustain human mesenchymal stem cell adhesion, proliferation, and differentiation, thus highlighting the possibility of using nanosheets as smart carriers of therapeutic cells.…”

Section: Cells On Magnetic Nanosheets and Their Guidancesupporting

confidence: 86%

See 1 more Smart Citation

Nanoscaffolds for Guided Cardiac Repair: The New Therapeutic Challenge of Regenerative Medicine

Ventrelli

Ricotti

Menciassi

et al. 2013

Journal of Nanomaterials

View full text Add to dashboard Cite

Cardiovascular diseases represent the leading cause of death and disability in the world. At the end-stage of heart failure, heart transplantation remains the ultimate option. Therefore, due to the numerous drawbacks associated with this procedure, new alternative strategies to repair the wounded heart are required. Cell therapy is a potential option to regenerate functional myocardial tissue. The characteristics of the ideal cardiac cell therapy include the use of the proper cell type and delivery methods as well as the choice of a suitable biomaterial acting as a cellular vehicle. Since traditional delivery methods are characterized by several counter backs, among which low cell survival, new engineered micro- and nanostructured materials are today extensively studied to provide a good cardiac therapy. In this review, we report the most recent achievements in the field of cell therapy for myocardial infarction treatment and heart regeneration, focusing on the most commonly used cell sources, the traditional approaches used to deliver cells at the damaged site, and a series of novel technologies based on recent advancements of bioengineering, highlighting the tremendous potential that nanoscaffolds have in this framework.

show abstract

Section: Cells On Magnetic Nanosheets and Their Guidancesupporting

confidence: 86%

“…In [97], Fujie et al reported the fabrication of two types (i.e., fibril and nonfibril) of free-standing ECM-like multilayer nanosheets made of biopolymers such as type I collagen (COL) and hyaluronic acid (HA). Both layer-bylayer (LbL) assembly method [98] and supporting technique [95,96] were used.…”

Section: Cells On Magnetic Nanosheets and Their Guidancementioning

confidence: 99%

Nanoscaffolds for Guided Cardiac Repair: The New Therapeutic Challenge of Regenerative Medicine

Ventrelli

Ricotti

Menciassi

et al. 2013

Journal of Nanomaterials

View full text Add to dashboard Cite

show abstract

“…For this reason, biomaterials with non‐thrombogenic features might improve the success rate when used in surface treatment of blood‐contacting devices in vivo . HA is a hydrophilic polysaccharide, which is present in a variety of native tissues (Ji et al ., ; Peppas et al ., ; Slaughter et al ., ; Suri and Schmidt, ; Fujie et al ., ; Lei et al ., ). Although HA is an abundant extracellular matrix (ECM) component in cardiovascular tissues, it is a non‐adhesive (Hu et al ., ; Leach et al ., ) substrate, limiting its application for cell spreading.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells

Camci‐Unal

Nichol

Bae

et al. 2012

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Endothelialization of artificial vascular grafts is a challenging process in cardiovascular tissue engineering. Functionalized biomaterials could be promising candidates to promote endothelialization in repair of cardiovascular injuries. The purpose of this study was to synthesize hyaluronic acid (HA) and heparin based hydrogels that could promote adhesion and spreading of endothelial progenitor cells (EPCs). We report that the addition of heparin into HA-based hydrogels provides an attractive surface for EPCs promoting spreading and the formation of an endothelial monolayer on the hydrogel surface. To increase EPC adhesion and spreading, we covalently immobilized CD34 antibody (Ab) on HA-heparin hydrogels using standard EDC/NHS amine coupling strategies. We found that EPC adhesion and spreading on CD34 Ab immobilized HA-heparin hydrogels was significantly higher than their nonmodified analogs. Once adhered, EPCs spread and formed an endothelial layer on both nonmodified and CD34 Ab modified HA-heparin hydrogels after 3 days of culture. We did not observe significant adhesion and spreading when heparin was not included in the control hydrogels. In addition to EPCs, we also used human umbilical cord vein endothelial cells (HUVECs), which adhered and spread on HA-heparin hydrogels. Macrophages exhibited significantly less adhesion compared to EPCs on the same hydrogels. This composite material could possibly be used to develop surface coatings for artificial cardiovascular implants, due to its specificity for EPC and endothelial cells on an otherwise non-thrombogenic surface.

show abstract

“…An interesting aspect of nanosheet is its noncovalent adhesiveness onto different substrates. Flat nanosheets have previously been demonstrated to adhere to the surface of substrates, such as Petri dishes and skin . [6a] Their noncovalent adhesiveness is exhibited by decreasing film thickness less than 200 nm due to the increment of film flexibility.…”

mentioning

confidence: 99%

Periosteum‐Mimetic Structures Made from Freestanding Microgrooved Nanosheets

et al. 2014

Self Cite

View full text Add to dashboard Cite

A "sticker-like" PLGA nanosheet with microgrooved patterns is developed through a facile combination of spin coating and micropatterning techniques. The resulting microgrooved PLGA nanosheets can be physically adhered on flat or porous surfaces with excellent stability in aqueous environments and can harness the spatial arrangements of cells, which make it a promising candidate for generating biomimic periosteum for bone regenerative applications.

show abstract

A nano-fibrous assembly of collagen–hyaluronic acid for controlling cell-adhesive properties

Cited by 29 publications

References 20 publications

Nanoscaffolds for Guided Cardiac Repair: The New Therapeutic Challenge of Regenerative Medicine

Nanoscaffolds for Guided Cardiac Repair: The New Therapeutic Challenge of Regenerative Medicine

Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells

Periosteum‐Mimetic Structures Made from Freestanding Microgrooved Nanosheets

Contact Info

Product

Resources

About