A Nano‐Electroporation‐DNA Tensioner Platform Enhances Intracellular Delivery and Mechanical Analysis Toward Rapid Drug Assessment
Xinxin Hang,
Zhaocun Huang,
Shiqi He
et al.
Abstract:In vitro, drug assessment holds tremendous potential to success in novel drug development and precision medicine. Traditional techniques for drug assessment, however, face remarkable challenges to achieve high speed, as limited by incubation‐based drug delivery (>several hours) and cell viability measurements (>1 d), which significantly compromise the efficacy in clinical trials. In this work, a nano‐electroporation‐DNA tensioner platform is reported that shortens the time of drug delivery to less than 3… Show more
“…1d). 59 Techniques involved in fabricating the required nanostructures include deep reactive ion etching (DRIE) for drilling nanochannels through silicon wafers, and track etching and femtosecond laser for creating nanopores in polymeric membranes. 60,61 The external electric field is concentrated through the nanostructures and exerted on a pinpoint in the cell membrane which is two magnitudes smaller in size than cellular diameters.…”
Section: Overview Of Micro/nano-electroporationmentioning
Gene therapy is a promising disease treatment approach by editing target genes, and thus plays a fundamental role in precision medicine. To ensure gene therapy efficacy, the effective delivery of...
“…1d). 59 Techniques involved in fabricating the required nanostructures include deep reactive ion etching (DRIE) for drilling nanochannels through silicon wafers, and track etching and femtosecond laser for creating nanopores in polymeric membranes. 60,61 The external electric field is concentrated through the nanostructures and exerted on a pinpoint in the cell membrane which is two magnitudes smaller in size than cellular diameters.…”
Section: Overview Of Micro/nano-electroporationmentioning
Gene therapy is a promising disease treatment approach by editing target genes, and thus plays a fundamental role in precision medicine. To ensure gene therapy efficacy, the effective delivery of...
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