A nano-biomimetic transformation system enables in planta expression of a reporter gene in mature plants and seeds

Abstract

Plant genetic engineering will be essential to decipher the genomic basis of complex traits, optimize crop genomics, and enable plant-based production of recombinant proteins. However, established plant transformation approaches for...

Nano-enabled agriculture: How do nanoparticles cross barriers in plants?

“…Leaf laminar infiltration ( Wu et al., 2017b ; Chincinska, 2021 ), foliar spraying ( Hong et al., 2014 ; Ma et al., 2020 ), vacuum infiltration ( Wu et al., 2017a ; Izuegbunam et al., 2021 ), and root application ( Zhang et al., 2019b ; Spielman-Sun et al., 2019 ) are the most commonly used approaches for delivering NPs into plants. Foliar application and root delivery are the main approaches used in agricultural practice.…”

An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays

“…Nanomaterials are increasingly being used for transient transformation. The most recent methods of nanoparticlemediated transformation or delivery of nucleic acids to plants have relied upon using pressure infiltration to deliver nanoparticles into plants via a needleless syringe (Zhang et al, 2019;Demirer et al, 2019;Izuegbunam et al, 2021;Zhang et al, 2022). Together, these nanoparticle-mediated methods have Workflow for VACNF mediated transient transformation of plants.…”

“…enabled delivery of nucleic acids and other molecules into Arabidopsis, tobacco, onion, maize, wheat, rapeseed, mustard, carrot, cotton, tomato, spinach, arugula, watercress, cowpea, chamomile, barley, and moss (Kumar et al, 2020;Jat et al, 2020;Izuegbunam et al, 2021). Roots can take up nanoparticles through passive diffusion and plant leaves can easily be transformed with passive infiltration of nanoparticles entering through the stomata (Hubbard et al, 2020).…”

“…To the knowledge of the authors, only one study previously used nanoparticles to transform other parts of a plants that were not leaves or roots. Specifically, in Izuegbunam et al (2021), the authors used needleless syringes to deliver nanorods with anchored DNA to developing seed tissues in Arabidopsis, field mustard, barley, and wheat. This method, like the aforementioned nanomaterialmediated delivery of DNA (Demirer et al, 2019;Jat et al, 2020;Zhang et al, 2022b), requires the conjugation of DNA to nanorods and subsequent release of DNA once inside cells.…”

“…The most recent methods of nanoparticle-mediated transformation or delivery of nucleic acids to plants have relied upon using pressure infiltration to deliver nanoparticles into plants via a needleless syringe ( Zhang et al., 2019 ; Demirer et al., 2019 ; Izuegbunam et al., 2021 ; Zhang et al., 2022 ). Together, these nanoparticle-mediated methods have enabled delivery of nucleic acids and other molecules into Arabidopsis, tobacco, onion, maize, wheat, rapeseed, mustard, carrot, cotton, tomato, spinach, arugula, watercress, cowpea, chamomile, barley, and moss ( Kumar et al., 2020 ; Jat et al., 2020 ; Izuegbunam et al., 2021 ). Roots can take up nanoparticles through passive diffusion and plant leaves can easily be transformed with passive infiltration of nanoparticles entering through the stomata ( Hubbard et al., 2020 ).…”